Opportunities for research via wheat transformation

Sparks, C. A., Martignago, D., Huttly, A. K., Raffan, S., Doherty, A. and Edwards, K. 2017. Opportunities for research via wheat transformation. The 5th Plant Genomics and Gene Editing Congress, Amsterdam, 16–17 March 2017. Societas Physiologiae Plantarum Scandinavica.

Related outputs

Stacked mutations in wheat homologues of rice SEMI-DWARF1 confer a novel semi-dwarf phenotype

A - Papers appearing in refereed journals

Ndreca, B., Huttly, A. K., Bibi, S., Bayon, C., Lund, G., Ham, J., Alarcon-Reverte, R., Addy, J., Tarkowská, D., Pearce, S., Hedden, P., Thomas, S. G. and Phillips, A. L. 2024. Stacked mutations in wheat homologues of rice SEMI-DWARF1 confer a novel semi-dwarf phenotype. BMC Plant Biology. 24, p. 384. https://doi.org/10.1186/s12870-024-05098-1

Low asparagine wheat - Europe’s first field trial of genome edited wheat amid rapidly changing regulations on acrylamide in food and genome editing of crops

A - Papers appearing in refereed journals

Kaur, N., Brock, T., Raffan, S. and Halford, N. G. 2024. Low asparagine wheat - Europe’s first field trial of genome edited wheat amid rapidly changing regulations on acrylamide in food and genome editing of crops. Breeding Science. 74, pp. 37-46. https://doi.org/10.1270/jsbbs.23058

A high soluble-fibre allele in wheat encodes a defective cell wall peroxidase responsible for dimerization of ferulate moieties on arabinoxylan

A - Papers appearing in refereed journals

Mitchell, R. A. C., Oszvald, M., Pellny, T. K., Freeman, J., Halsey, K., Sparks, C. A., Huttly, A. K., Specel, S., Leverington-Waite, M., Griffiths, S., Shewry, P. R. and Lovegrove, A. 2023. A high soluble-fibre allele in wheat encodes a defective cell wall peroxidase responsible for dimerization of ferulate moieties on arabinoxylan. bioRxiv. https://doi.org/10.1101/2023.03.08.531735

Field assessment of genome edited, low asparagine wheat: Europe's first CRISPR wheat field trial.

A - Papers appearing in refereed journals

Raffan, S., Oddy, J., Mead, A., Barker, G., Curtis, T. Y., Usher, S. L., Burt, C. and Halford, N. G. 2023. Field assessment of genome edited, low asparagine wheat: Europe's first CRISPR wheat field trial. Plant Biotechnology Journal. 21 (6), pp. 1097-1099. https://doi.org/10.1111/pbi.14026

Reducing dietary acrylamide exposure from wheat products through crop management and imaging

A - Papers appearing in refereed journals

Oddy, J., Addy, J., Mead, A., Hall, C., Mackay, C., Ashfield, T., Mcdiarmid, F., Curtis, T. Y., Raffan, S., Wilkinson, M. D., Elmore, J. S., Cryer, N., Moreira de Almeida, I. and Halford, N. G. 2023. Reducing dietary acrylamide exposure from wheat products through crop management and imaging. Journal of Agricultural and Food Chemistry - JAFC. 71 (7), pp. 3403-3413. https://doi.org/10.1021/acs.jafc.2c07208

Genetic control of grain amino acid composition in a UK soft wheat mapping population

A - Papers appearing in refereed journals

Oddy, J., Chhetry, M., Awal, R., Addy, J., Wilkinson, M. D., Smith, D., King, R., Hall, C., Testa, R., Murray, E., Raffan, S., Curtis, T. Y., Wingen, L., Griffiths, S., Berry, S., Elmore, S. J., Cryer, N., Moreira de Almeida, I. and Halford, N. G. 2023. Genetic control of grain amino acid composition in a UK soft wheat mapping population. The Plant Genome. 16 (4), p. e20335. https://doi.org/10.1002/tpg2.20335

Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants

A - Papers appearing in refereed journals

Hyde, L., Osman, K., Winfield, M., Sanchez-Moran, E., Higgins, J. D., Henderson, I. R., Sparks, C. A., Franklin, F. C. H. and Edwards, K. 2022. Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants. Plant Biotechnology Journal. 2022, pp. 1-4. https://doi.org/10.1111/pbi.13961

Proanthocyanidin Biosynthesis in the Developing Wheat Seed Coat Investigated by Chemical and RNA-seq Analysis

A - Papers appearing in refereed journals

Phillips, A. L., Vaughan, S., Baker, J., Primavesi, L. F., Patil, A., King, R., Hassani-Pak, K., Kulasekaran, S., Coghill, J., Ward, J. L. and Huttly, A. K. 2022. Proanthocyanidin Biosynthesis in the Developing Wheat Seed Coat Investigated by Chemical and RNA-seq Analysis. Plant Direct. 6 (10), p. e453. https://doi.org/10.1002/pld3.453

Epigenetic switch reveals CRISPR/Cas9 response to cytosine methylation in plants: Comment

A - Papers appearing in refereed journals

Raffan, S., Kaur, N. and Halford, N. G. 2022. Epigenetic switch reveals CRISPR/Cas9 response to cytosine methylation in plants: Comment. New Phytologist. 235 (6), pp. 2146-2148. https://doi.org/10.1111/nph.18405

Progress towards the production of potatoes and cereals with low acrylamide-forming potential

A - Papers appearing in refereed journals

Halford, N. G., Raffan, S. and Oddy, J. 2022. Progress towards the production of potatoes and cereals with low acrylamide-forming potential. Current Opinion in Food Science. 47, p. 100887. https://doi.org/10.1016/j.cofs.2022.100887

Understanding the Relationships between Free Asparagine in Grain and Other Traits to Breed Low-Asparagine Wheat

A - Papers appearing in refereed journals

Oddy, J., Raffan, S., Wilkinson, M. D., Elmore, J. S. and Halford, N. G. 2022. Understanding the Relationships between Free Asparagine in Grain and Other Traits to Breed Low-Asparagine Wheat. Plants - Basel. 11 (5), p. 669. https://doi.org/10.3390/plants11050669

Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

A - Papers appearing in refereed journals

Hahn, F., Sanjurjo-Loures, L., Sparks, C. A., Kanyuka, K. and Nekrasov, V. 2021. Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties. Plants - Basel. 10 (7), p. 1481. https://doi.org/10.3390/plants10071481

Reduced free asparagine in wheat grain resulting from a natural deletion of TaASNB2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality

A - Papers appearing in refereed journals

Oddy, J., Alarcon-Reverte, R., Wilkinson, M. D., Ravet, K., Raffan, S., Minter, A., Mead, A., Elmore, J. S., Moreira de Almeida, I., Cryer, N., Halford, N. G. and Pearce, S. 2021. Reduced free asparagine in wheat grain resulting from a natural deletion of TaASNB2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality. BMC Plant Biology. 21 (1), p. 302. https://doi.org/10.1186/s12870-021-03058-7

Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2

A - Papers appearing in refereed journals

Raffan, S., Sparks, C. A., Huttly, A. K., Hyde, L., Martignago, D., Mead, A., Hanley, S. J., Wilkinson, P., Barker, G., Edwards, K. J., Curtis, T. Y., Usher, S. L., Kosik, O. and Halford, N. G. 2021. Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2 . Plant Biotechnology Journal. 19 (8), pp. 1602-1613. https://doi.org/10.1111/pbi.13573

Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain

A - Papers appearing in refereed journals

Wan, Y., Wang, Y., Shi, Z., Rentsch, D., Ward, J. L., Hassall, K. L., Sparks, C. A., Huttly, A. K., Buchner, P. H., Powers, S. J., Shewry, P. R. and Hawkesford, M. J. 2021. Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain. PLOS ONE. 16 (2), p. e0246763. https://doi.org/10.1371/journal.pone.0246763

Genome Editing for Low-Acrylamide Wheat

F - Theses

Raffan, S. 2020. Genome Editing for Low-Acrylamide Wheat. F - Theses

Stress, nutrients and genotype - understanding and managing asparagine accumulation in wheat grain.

A - Papers appearing in refereed journals

Oddy, J., Raffan, S., Wilkinson, M. D., Elmore, J. S. and Halford, N. G. 2020. Stress, nutrients and genotype - understanding and managing asparagine accumulation in wheat grain. CABI Agriculture and Bioscience. 1, p. 10. https://doi.org/10.1186/s43170-020-00010-x

Cereal asparagine synthetase genes

A - Papers appearing in refereed journals

Raffan, S. and Halford, N. G. 2020. Cereal asparagine synthetase genes. Annals of Applied Biology - AAB. https://doi.org/10.1111/aab.12632

CRISPR-Cas9 multiplex editing of the α-amylase/trypsin inhibitor genes to reduce allergen proteins in durum wheat

A - Papers appearing in refereed journals

Camerlengo, F., Frittelli, A., Sparks, C. A., Doherty, A., Martignago, D., Larre, C., Lupi, R., Sestili, F. and Masci, S. 2020. CRISPR-Cas9 multiplex editing of the α-amylase/trypsin inhibitor genes to reduce allergen proteins in durum wheat. Frontiers in Sustainable Food Systems. https://doi.org/10.3389/fsufs.2020.00104

The sulphur response in wheat and its implications for acrylamide formation and food safety

A - Papers appearing in refereed journals

Raffan, S., Oddy, J. and Halford, N. G. 2020. The sulphur response in wheat and its implications for acrylamide formation and food safety. International Journal Of Molecular Sciences. 21 (11), p. 3876. https://doi.org/10.3390/ijms21113876

Genetic transformation of common wheat (Triticum aestivum L.) using biolistics

B - Book chapters etc edited externally

Sparks, C. A. and Doherty, A. 2020. Genetic transformation of common wheat (Triticum aestivum L.) using biolistics. in: Rustgi, S. and Luo, H. (ed.) Biolistic DNA Delivery in Plants New York Humana Press Inc.

Reducing the acrylamide-forming potential of crop plants

B - Book chapters etc edited externally

Raffan, S. and Halford, N. G. 2019. Reducing the acrylamide-forming potential of crop plants. in: Jaiwal, P. K., Chhillar, A. K., Chaudhary, D. and Jaiwal, R. (ed.) Nutritional quality improvement in plants Basel, Switzerland Springer Nature.

Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat

A - Papers appearing in refereed journals

Lobo, A. K. M., Orr, D., Gutierrez, M. O., Andralojc, P. J., Sparks, C. A., Afan, M., Parry, J., Parry, M. A. J. and Carmo-Silva, E. 2019. Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat. Plant Physiology. 181 (October), pp. 471-479. https://doi.org/10.1104/pp.19.00693

Contrasting gene expression patterns in grain of high and low asparagine wheat genotypes in response to sulphur supply

A - Papers appearing in refereed journals

Curtis, T., Raffan, S., Wan, Y., King, R., Gonzalez-Uriarte, A. and Halford, N. G. 2019. Contrasting gene expression patterns in grain of high and low asparagine wheat genotypes in response to sulphur supply. BMC Genomics. 20, p. 628. https://doi.org/10.1186/s12864-019-5991-8

Acrylamide in food: progress in and prospects for genetic and agronomic solutions

A - Papers appearing in refereed journals

Raffan, S. and Halford, N. G. 2019. Acrylamide in food: progress in and prospects for genetic and agronomic solutions. Annals of Applied Biology - AAB. 175 (3), pp. 259-281. https://doi.org/10.1111/aab.12536

Use of the pre-assembled ribonucleoprotein (RNP) complex for gene editing of wheat and poplar

Conference poster

Sparks, C. A., Cerezo-Medina, S., Raffan, S. and Hahn, F. 2019. Use of the pre-assembled ribonucleoprotein (RNP) complex for gene editing of wheat and poplar. The 7th Plant Genomics and Gene Editing Congress. Rotterdam, The Netherlands 21 May 2019

Silencing an essential gene involved in infestation and digestion in grain aphid through plant‐mediated RNA interference generates aphid‐resistant wheat plants

A - Papers appearing in refereed journals

Sun, Y., Sparks, C. A., Jones, H., Riley, M., Francis, F., Du, W. and Xia, L. 2019. Silencing an essential gene involved in infestation and digestion in grain aphid through plant‐mediated RNA interference generates aphid‐resistant wheat plants. Plant Biotechnology Journal. 17 (5), pp. 852-854. https://doi.org/10.1111/pbi.13067

Acrylamide: new European risk management measures and prospects for reducing the acrylamide-forming potential of wheat

B - Book chapters etc edited externally

Halford, N. G. and Raffan, S. 2019. Acrylamide: new European risk management measures and prospects for reducing the acrylamide-forming potential of wheat. in: Food-Borne Toxicants: Formation, Analysis, and Toxicology Washington DC American Chemical Society (ACS). pp. Chapter 3, pp 27–43

Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici

A - Papers appearing in refereed journals

Kettles, G. J., Bayon, C., Sparks, C. A., Canning, G., Kanyuka, K. and Rudd, J. J. 2018. Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici. New Phytologist. 217 (1), pp. 320-331. https://doi.org/10.1111/nph.14786

Identification of Leaf Promoters for Use in Transgenic Wheat

A - Papers appearing in refereed journals

Alotaibi, S. S., Sparks, C. A., Parry, M. A. J., Simkin, A. J. and Raines, C. A. 2018. Identification of Leaf Promoters for Use in Transgenic Wheat. Plants - Basel. 7 (2), p. 27. https://doi.org/10.3390/plants7020027

Genomic, biochemical and modelling analyses of asparagine synthetases from wheat

A - Papers appearing in refereed journals

Xu, H., Curtis, T. Y., Powers, S. J., Raffan, S., Gao, R., Huang, J., Heiner, M., Gilbert, D. R. and Halford, N. G. 2018. Genomic, biochemical and modelling analyses of asparagine synthetases from wheat. Frontiers in Plant Science. 8, p. 2237. https://doi.org/10.3389/fpls.2017.02237

Host-induced gene silencing for the control of the Fusarium Head Blight in wheat fields

C2 - Non-edited contributions to conferences

Kanyuka, K., Machado, A., Urban, M., Lee, W. S., Brown, N. J., King, R. A., Lau, E. Y., Sparks, C. A., Bonato, A. L. V., Tibola, C. S., Martins, N. F., Aragao, F. J., West, J., Fernandes, J. M. C. and Hammond-Kosack, K. E. 2017. Host-induced gene silencing for the control of the Fusarium Head Blight in wheat fields. 1st COST iPlanta Conferernce; "Creating the plant RNAi research network CA15223", La Sapienza - CNR Headquarter , Rome, Italy, 15-17 February 2017.

Antagonistic Transcription Factor Complexes Modulate the Floral Transition in Rice

A - Papers appearing in refereed journals

Brambilla, V., Martignago, D., Goretti, D., Cerise, M., Somssich, M., de Rosa, M., Galbiati, F., Shrestha, R., Lazzaro, F., Simon, R. and Fornara, F. 2017. Antagonistic Transcription Factor Complexes Modulate the Floral Transition in Rice. The Plant Cell. 29 (11), pp. 2801-2816. https://doi.org/10.1105/tpc.17.00645

Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions

A - Papers appearing in refereed journals

Driever, S. M., Simkin, A. J., Alotaibi, S., Fisk, S. J., Madgwick, P. J., Sparks, C. A., Jones, H. D., Lawson, T., Parry, M. A. J. and Raines, C. A. 2017. Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions. Philosophical Transactions of the Royal Society B-Biological Sciences. 372 (1730), pp. 1-10. https://doi.org/10.1098/rstb.2016.0384

Use of a modified CRISPR/Cas9 construct to simultaneously mutagenize multiple genes in hexaploid wheat

C1 - Edited contributions to conferences/learned societies

Winfield, M., Allen, S., Cartlidge, L., Sparks, C. A., Jones, H., Huttly, A. K., Martignago, D., Hawkesford, M. J. and Edwards, K. 2017. Use of a modified CRISPR/Cas9 construct to simultaneously mutagenize multiple genes in hexaploid wheat. MonoGram 2017, 4-6 April, University of Bristol. Bristol, University of Bristol.

Triticum aestivum cultivar Spark asparagine synthetase-1 (ASN1) mRNA, complete cds

K - Protein or DNA sequences submitted to database

Xu, H., Curtis, T. Y., Powers, S. J., Raffan, S., Gao, R., Huang, J., Heiner, M., Gilbert, D. and Halford, N. G. 2017. Triticum aestivum cultivar Spark asparagine synthetase-1 (ASN1) mRNA, complete cds.

Secale cereale glutamine synthetase-1 (GS1-1) mRNA, partial cds

K - Protein or DNA sequences submitted to database

Curtis, T., Halford, N. G. and Raffan, S. 2017. Secale cereale glutamine synthetase-1 (GS1-1) mRNA, partial cds.

Triticum aestivum cultivar Spark asparagine synthetase-3 (ASN3) mRNA, complete cds

K - Protein or DNA sequences submitted to database

Xu, H., Curtis, T. Y., Powers, S. J., Raffan, S., Gao, R., Huang, J., Heiner, M., Gilbert, D. and Halford, N. G. 2017. Triticum aestivum cultivar Spark asparagine synthetase-3 (ASN3) mRNA, complete cds.

Wheat biotechnology: recent developments and future trends

B - Book chapters etc edited externally

Sparks, C. A. 2016. Wheat biotechnology: recent developments and future trends. in: Jones, H. D. (ed.) Biotechnology of major cereals Chapter 6 CABI International, Wallingford, Oxon (CABI). pp. 88-103

The first crop plant genetically engineered to release an insect pheromone for defence

A - Papers appearing in refereed journals

Bruce, T. J. A., Aradottir, G. I., Smart, L. E., Martin, J. L., Caulfield, J. C., Doherty, A., Sparks, C. A., Woodcock, C. M., Birkett, M. A., Napier, J. A., Jones, H. D. and Pickett, J. A. 2015. The first crop plant genetically engineered to release an insect pheromone for defence. Scientific Reports. 5, p. 11183. https://doi.org/10.1038/srep11183

Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family

A - Papers appearing in refereed journals

Pearce, S., Huttly, A. K., Prosser, I. M., Li, Y-D., Vaughan, S. P., Gallova, B., Patil, A., Coghill, J. A., Dubcovsky, J., Hedden, P. and Phillips, A. L. 2015. Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family. BMC Plant Biology. 15, p. 130. https://doi.org/10.1186/s12870-015-0520-7

Genetic transformation of wheat via particle bombardment

B - Book chapters etc edited externally

Sparks, C. A. and Jones, H. D. 2014. Genetic transformation of wheat via particle bombardment. in: Henry, R. J. and Furtado, A. (ed.) Cereal genomics: methods and protocols. Methods in Molecular Biology, vol 1099 Springer, New York. pp. 201-218

Genetic transformation of wheat via Agrobacterium-mediated DNA delivery

B - Book chapters etc edited externally

Sparks, C. A., Doherty, A. and Jones, H. D. 2014. Genetic transformation of wheat via Agrobacterium-mediated DNA delivery. in: Henry, R. J. and Furtado, A. (ed.) Cereal genomics: methods and protocols. Methods in Molecular Biology, vol 1099 Springer, New York. pp. 235-250

Modelling wheat breakage during roller milling using the Double Normalised Kumaraswamy Breakage Function: effects of kernel shape and hardness

A - Papers appearing in refereed journals

Campbell, G. M., Sharp, C., Wall, K., Mateos-Salvador, F., Gubatz, S., Huttly, A. K. and Shewry, P. R. 2012. Modelling wheat breakage during roller milling using the Double Normalised Kumaraswamy Breakage Function: effects of kernel shape and hardness. Journal of Cereal Science. 55, pp. 415-425. https://doi.org/10.1016/j.jcs.2012.02.002

Temporal and spatial transgene expression using a heat-shock promoter in transgenic wheat

C1 - Edited contributions to conferences/learned societies

Freeman, J., Sparks, C. A., West, J. S., Shewry, P. R. and Jones, H. D. 2011. Temporal and spatial transgene expression using a heat-shock promoter in transgenic wheat. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 85-91

Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene

C1 - Edited contributions to conferences/learned societies

Bruce, T. J. A., Smart, L. E., Martin, J. L., Doherty, A., Jones, H. D., Napier, J. A. and Pickett, J. A. 2011. Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 24

Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene

C1 - Edited contributions to conferences/learned societies

Bruce, T. J. A., Smart, L. E., Aradottir, G. I., Martin, J. L., Doherty, A., Jones, H. D., Napier, J. A. and Pickett, J. A. 2011. Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 112

The role of gibberellin in determining wheat grain size and quality

C2 - Non-edited contributions to conferences

Vaughan, S. P., Wanchoo-Kohli, A., Patil, A., Huttly, A. K., Phillips, A. L. and Hedden, P. 2011. The role of gibberellin in determining wheat grain size and quality. ABRATES.

The genetics and biochemistry of wheat grain colour

C2 - Non-edited contributions to conferences

Vaughan, S. P., Baker, J., Ward, J. L., Huttly, A. K. and Phillips, A. L. 2011. The genetics and biochemistry of wheat grain colour. ABRATES.

The role of gibberellin in determining wheat grain size and quality

C1 - Edited contributions to conferences/learned societies

Vaughan, S. P., Wanchoo-Kohli, A., Patil, A., Huttly, A. K., Phillips, A. L. and Hedden, P. 2011. The role of gibberellin in determining wheat grain size and quality. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 14

Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene

C2 - Non-edited contributions to conferences

Bruce, T. J. A., Smart, L. E., Aradottir, G. I., Martin, J. L., Doherty, A., Jones, H. D., Napier, J. A. and Pickett, J. A. 2011. Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene. Abstracts Ento' 11 Symposium on Chemical Ecology: Reception, Detection and Deception, Chatham, 7-9 September 2011 . pp. 54

Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat

A - Papers appearing in refereed journals

Pearce, S., Saville, R., Vaughan, S. P., Chandler, P. M., Wilhelm, E. P., Sparks, C. A., Al-Kaff, N., Korolev, A., Boulton, M. I., Phillips, A. L., Hedden, P., Nicholson, P. and Thomas, S. G. 2011. Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat. Plant Physiology. 157, pp. 1820-1831. https://doi.org/10.1104/pp.111.183657

Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat

A - Papers appearing in refereed journals

Freeman, J., Sparks, C. A., West, J., Shewry, P. R. and Jones, H. D. 2011. Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotechnology Journal. 9 (7), pp. 788-796. https://doi.org/10.1111/j.1467-7652.2011.00588.x

Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging

C1 - Edited contributions to conferences/learned societies

Tosi, P., Gritsch, C., Freeman, J., Sparks, C. A., Jones, H. D., Funatsuki, W., Niwa, K. and Shewry, P. R. 2010. Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging. Branlard, G. (ed.) Gluten Proteins 2009: Proceedings 10th International Gluten Workshop, Clermont-Ferrand, 7-9 September 2009 . Institut National de la Recherche Agronomique (INRA), Paris. pp. 17-20

Dough properties of transgenic wheat transformed with Cys-mutated low-molecular-weight glutenin genes

C1 - Edited contributions to conferences/learned societies

Funatsuki, W., Tosi, P., Ito, M., Jones, H. D., Sparks, C. A., Riley, A., Nishio, Z., Tadashi, T., Yamauchi, H. and Shewry, P. R. 2010. Dough properties of transgenic wheat transformed with Cys-mutated low-molecular-weight glutenin genes. Branlard, G. (ed.) Gluten Proteins 2009: Proceedings of the 10th International Gluten Workshop, Clermont-Ferrand, 7-9 September 2009 . Institut National de la Recherche Agronomique (INRA), Paris. pp. 175-179

Increasing the amylose content of durum wheat through silencing of the SBE11a genes

A - Papers appearing in refereed journals

Sestili, F., Janni, M., Doherty, A., Botticella, E., D'ovidio, R., Masci, S., Jones, H. D. and Lafiandra, D. 2010. Increasing the amylose content of durum wheat through silencing of the SBE11a genes. BMC Plant Biology. 10, p. 144 (12pp). https://doi.org/10.1186/1471-2229-10-144

Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat

A - Papers appearing in refereed journals

Nemeth, C., Freeman, J., Jones, H. D., Sparks, C. A., Pellny, T. K., Wilkinson, M. D., Dunwell, J., Andersson, A. A. M., Aman, P., Guillon, F., Saulnier, L., Mitchell, R. A. C. and Shewry, P. R. 2010. Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat. Plant Physiology. 152, pp. 1209-1218. https://doi.org/10.1104/pp.109.151712

Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging

C2 - Non-edited contributions to conferences

Tosi, P., Gritsch, C., Freeman, J., Sparks, C. A., Jones, H. D., Funatsuki, W., Niwa, K. and Shewry, P. R. 2009. Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging. Abstracts Xth International Gluten Workshop, Clermont Ferrand, 7-9 September 2009 .

Stable transformation of plants

B - Book chapters etc edited externally

Jones, H. D. and Sparks, C. A. 2009. Stable transformation of plants. in: Gustafson, J. P., Somers, D. J. and Langridge, P. (ed.) Plant genomics. (Methods in Molecular Biology vol 513) Humana Press, Totowa, New Jersey. pp. 111-130

Transient transformation of plants

B - Book chapters etc edited externally

Jones, H. D., Doherty, A. and Sparks, C. A. 2009. Transient transformation of plants. in: Gustafson, J. P., Somers, D. J. and Langridge, P. (ed.) Plant genomics. (Methods in Molecular Biology vol 513) Humana Press, Totowa, New Jersey. pp. 131-152

Transgenic manipulation of wheat quality

B - Book chapters etc edited externally

Jones, H. D. and Sparks, C. A. 2009. Transgenic manipulation of wheat quality. in: Shewry, P. R. and Khan, K. (ed.) Wheat: chemistry and technology, 4th ed American Association of Cereal Chemists (AACC), St Paul, MN. pp. 437-451

A transgenic approach to understanding gene expression in cereals

C1 - Edited contributions to conferences/learned societies

Jones, H. D., Freeman, J. and Sparks, C. A. 2008. A transgenic approach to understanding gene expression in cereals. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 427-430

Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm

C1 - Edited contributions to conferences/learned societies

Freeman, J., Kurup, S., Huttly, A. K. and Shewry, P. R. 2008. Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 453-455

Reporter genes

B - Book chapters etc edited externally

Huttly, A. K. 2008. Reporter genes. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 39-69

Agrobacterium- mediated transformation of bread and durum wheat using freshly isolated immature embryos

B - Book chapters etc edited externally

Wu, H. and Doherty, A. 2008. Agrobacterium- mediated transformation of bread and durum wheat using freshly isolated immature embryos. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 93-103

Biolistics transformation of wheat

B - Book chapters etc edited externally

Sparks, C. A. 2008. Biolistics transformation of wheat. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 71-92

Selection of transformed plants

B - Book chapters etc edited externally

Sparks, C. A. 2008. Selection of transformed plants. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 23-37

Promoter sequences for defining transgene expression

B - Book chapters etc edited externally

Sparks, C. A. 2008. Promoter sequences for defining transgene expression. in: Shewry, P. R. and Jones, H. D. (ed.) In %Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 171-184

Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development

A - Papers appearing in refereed journals

Van Herpen, T. W. J. M., Riley, M., Sparks, C. A., Jones, H. D., Gritsch, C., Dekking, E. H., Hamer, R. J., Bosch, D., Salentijn, E. M. J., Smulders, M. J. M., Shewry, P. R. and Gilissen, L. J. W. J. 2008. Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development. Annals of Botany. 102, pp. 331-342. https://doi.org/10.1093/aob/mcn114

Transcriptome analysis of grain development in hexaploid wheat

A - Papers appearing in refereed journals

Wan, Y., Poole, R. L., Huttly, A. K., Toscano-Underwood, C., Feeney, K., Welham, S. J., Gooding, M. J., Mills, C., Edwards, K. J., Shewry, P. R. and Mitchell, R. A. C. 2008. Transcriptome analysis of grain development in hexaploid wheat. BMC Genomics. 9, p. 121 (16pp). https://doi.org/10.1186/1471-2164-9-121

Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes

A - Papers appearing in refereed journals

Wu, H., Doherty, A. and Jones, H. D. 2008. Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes. Transgenic Research. 17, pp. 425-436. https://doi.org/10.1007/s11248-007-9116-9

The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations

A - Papers appearing in refereed journals

Pastori, G. M., Huttly, A. K., West, J., Sparks, C. A., Pieters, A., Luna, C. M., Jones, H. D. and Foyer, C. H. 2007. The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations. Functional Plant Biology. 34 (9), pp. 835-843. https://doi.org/10.1071/FP07112

Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm

C2 - Non-edited contributions to conferences

Freeman, J., Kurup, S., Huttly, A. K. and Shewry, P. R. 2007. Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm. Abstracts 4th UK Small Grain Cereals Genetic and Genomics Workshop: From Genes to Traits, Cambridge, 14-16 March 2007 .

High throughput Agrobacterium transformation of wheat: a tool for functional genomics

C1 - Edited contributions to conferences/learned societies

Jones, H. D., Wilkinson, M. D., Doherty, A. and Wu, H. 2007. High throughput Agrobacterium transformation of wheat: a tool for functional genomics. Buck, H. T., Salomon, N. and Nisi, J. E. (ed.) Wheat Production in Stressed Environments: Proceedings 7th International Wheat Conference, Mar del Plata, Argentina, 27 November-2 December 2005 . Springer, Dordrecht. pp. 693-699

Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength

C2 - Non-edited contributions to conferences

Maruyama-Funatsuki, W., Tosi, P., Jones, H. D., Sparks, C. A., Riley, A., Yamauchi, H. and Shewry, P. R. 2007. Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength.

Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins

C1 - Edited contributions to conferences/learned societies

Shewry, P. R., Tosi, P., Jones, H. D., Sparks, C. A., Gritsch, C., Funatsuki, W., Niwa, K., Huttly, A. K., Napier, J. A., D'ovidio, R. and Freeman, J. 2007. Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins. Lookhart, G. L. and Ng, P. W. K. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 112-116

Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation

A - Papers appearing in refereed journals

Wu, H., Sparks, C. A. and Jones, H. D. 2006. Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation. Molecular Breeding. 18, pp. 195-208. https://doi.org/10.1007/s11032-006-9027-0

Aphid alarm pheromone produced by transgenic plants affects aphid and parasitoid behavior

A - Papers appearing in refereed journals

Beale, M. H., Birkett, M. A., Bruce, T. J. A., Chamberlain, K., Field, L. M., Huttly, A. K., Martin, J. L., Parker, R., Phillips, A. L., Pickett, J. A., Prosser, I. M., Shewry, P. R., Smart, L. E., Wadhams, L. J., Woodcock, C. M. and Zhang, Y. 2006. Aphid alarm pheromone produced by transgenic plants affects aphid and parasitoid behavior. Proceedings of the National Academy of Sciences. 103, pp. 10509-10513. https://doi.org/10.1073/pnas.0603998103

Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat

A - Papers appearing in refereed journals

Jones, H. D., Doherty, A. and Wu, H. 2005. Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat. Plant Methods. 1:5. https://doi.org/10.1186/1746-4811-1-5

Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25

A - Papers appearing in refereed journals

Howarth, J. R., Jacquet, J. N., Doherty, A., Jones, H. D. and Cannell, M. E. 2005. Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25. Annals of Applied Biology - AAB. 146 (3), pp. 311-320. https://doi.org/10.1111/j.1744-7348.2005.040121.x

Transformation of wheat by biolistics

B - Book chapters etc edited externally

Sparks, C. A. and Jones, H. D. 2004. Transformation of wheat by biolistics. in: Curtis, I. S. (ed.) Transgenic crops of the world: essential protocols Kluwer Academic Publishers, Dordrecht, Netherlands. pp. 19-33

Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

A - Papers appearing in refereed journals

Mendoza De Gyves, E., Sparks, C. A., Sayanova, O. V., Lazzeri, P. A., Napier, J. A. and Jones, H. D. 2004. Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.). Plant Biotechnology Journal. 2 (4), pp. 351-357. https://doi.org/10.1111/j.1467-7652.2004.00079.x

The potential of biotechnology to produce novel gluten phenotypes

C1 - Edited contributions to conferences/learned societies

Jones, H. D., Wu, H., Sparks, C. A., Doherty, A., Cannell, M., Goodwin, J., Pastori, G. M. and Shewry, P. R. 2004. The potential of biotechnology to produce novel gluten phenotypes. D'ovidio, R., Lafiandra, D. and Masci, S. (ed.) Gluten Proteins. Proceedings of the 8th Gluten Workshop, Viterbo, 8-10 September 2003 . Royal Society of Chemistry (RSC), Cambridge. pp. 14-17

Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat

C2 - Non-edited contributions to conferences

Doherty, A., West, J. M., Sparks, C. A., Stone, M. C., Cannell, M. and Jones, H. D. 2003. Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 .

Transgenic approaches to study quality traits in cereals

C1 - Edited contributions to conferences/learned societies

Jones, H. D., Wu, H., Sparks, C. A. and Shewry, P. R. 2003. Transgenic approaches to study quality traits in cereals. Proceedings BCPC International Congress - Crop Science and Technology, Glasgow, 10-12 November 2003 . pp. 133-140

CLAVATA1 dominant-negative alleles reveal functional overlap between multiple receptor kinases that regulate meristem and organ development

A - Papers appearing in refereed journals

Dievert, A., Dalal, M., Tax, F. E., Lacey, A. D., Huttly, A. K., Li, J. and Clark, S. E. 2003. CLAVATA1 dominant-negative alleles reveal functional overlap between multiple receptor kinases that regulate meristem and organ development. The Plant Cell. 15 (5), pp. 1198-1211. https://doi.org/10.​1105/​tpc.​010504

Promoters for driving transgene expression in wheat

C1 - Edited contributions to conferences/learned societies

Sparks, C. A., Collins, R. and Jones, H. D. 2003. Promoters for driving transgene expression in wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 .

Optimisation factors for Agrobacterium -mediated genetic transformation of wheat (cv Florida)

C1 - Edited contributions to conferences/learned societies

Wu, H., Sparks, C. A. and Jones, H. D. 2003. Optimisation factors for Agrobacterium -mediated genetic transformation of wheat (cv Florida). Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 . pp. 181-184

Agrobacterium mediated transformation of wheat: progress and application

C1 - Edited contributions to conferences/learned societies

Jones, H. D., Wu, H., Patil, M. S., Rubio, S. and Sparks, C. A. 2003. Agrobacterium mediated transformation of wheat: progress and application. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 . pp. 914-916

Factors influencing successful Agrobacterium -mediated genetic transformation of wheat

A - Papers appearing in refereed journals

Wu, H., Sparks, C. A., Amoah, B. and Jones, H. D. 2003. Factors influencing successful Agrobacterium -mediated genetic transformation of wheat. Plant Cell Reports. 21, pp. 659-668.

Novel anther-specific myb genes from tobacco as putative regulators of phenylalanine ammonia-lyase expression

A - Papers appearing in refereed journals

Yang, S., Sweetman, J. P., Amirsadeghi, S., Barghchi, M., Huttly, A. K., Chung, W-I. and Twell, D. 2001. Novel anther-specific myb genes from tobacco as putative regulators of phenylalanine ammonia-lyase expression. Plant Physiology. 126, pp. 1738-1753.

Age-dependent transformation frequency in elite wheat varieties

A - Papers appearing in refereed journals

Pastori, G. M., Wilkinson, M. D., Steele, S. H., Sparks, C. A., Jones, H. D. and Parry, M. A. J. 2001. Age-dependent transformation frequency in elite wheat varieties. Journal of Experimental Botany. 52, pp. 857-863.

High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron

A - Papers appearing in refereed journals

Mendoza De Gyves, E., Sparks, C. A., Fieldsend, A. F., Lazzeri, P. A. and Jones, H. D. 2001. High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron. Annals of Applied Biology - AAB. 138 (3), pp. 329-332.

Factors influencing Agrobacterium -mediated transient expression of uid A in wheat inflorescence tissue

A - Papers appearing in refereed journals

Amoah, B. K., Wu, H., Sparks, C. A. and Jones, H. D. 2001. Factors influencing Agrobacterium -mediated transient expression of uid A in wheat inflorescence tissue. Journal of Experimental Botany. 52, pp. 1135-1142.

Hormonal and developmental regulation of alpha-amylase in transgenic wheat

C2 - Non-edited contributions to conferences

Stone, M. C., Wilkinson, M. D., Huttly, A. K., Lenton, J. R. and Jones, H. D. 2001. Hormonal and developmental regulation of alpha-amylase in transgenic wheat. Abstracts SEB Annual Meeting, Canterbury, 2-6 April 2001 . pp. P7.78

Potential for manipulating carbon metabolism in wheat

A - Papers appearing in refereed journals

Sparks, C. A., Castleden, C. K., West, J., Habash, D. Z., Madgwick, P. J., Paul, M. J., Noctor, G., Harrison, J., Wu, R., Wilkinson, J., Quick, W. P., Parry, M. A. J., Foyer, C. H. and Miflin, B. J. 2001. Potential for manipulating carbon metabolism in wheat. Annals of Applied Biology - AAB. 138 (1), pp. 33-45. https://doi.org/10.1111/j.1744-7348.2001.tb00083.x

Overexpression of sucrose-phosphate synthase in wheat

C2 - Non-edited contributions to conferences

Castleden, C. K., Sparks, C. A., West, J. M., Quick, W. P. and Foyer, C. H. 1999. Overexpression of sucrose-phosphate synthase in wheat. Abstracts International Conference on Assimilate Transport and Partitioning, Newcastle NSW, 15-20 August 1999 . pp. 95

A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability

A - Papers appearing in refereed journals

Cannell, M. E., Doherty, A., Lazzeri, P. A. and Barcelo, P. 1999. A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability. Theoretical and Applied Genetics. 99, pp. 772-784.

Current status of transformationin bread and durum wheats and modifications of gluten quality

C1 - Edited contributions to conferences/learned societies

He, G. Y., Rooke, L., Cannell, M., Rasco-Gaunt, S., Sparks, C. A., Lamacchia, C., Bekes, F., Tatham, A. S., Barcelo, P., Shewry, P. R. and Lazzeri, P. A. 1998. Current status of transformationin bread and durum wheats and modifications of gluten quality.

Isolation and Expression of Three Gibberellin 20-Oxidase cDNA Clones from Arabidopsis

A - Papers appearing in refereed journals

Phillips, A. L., Ward, D. A., Uknes, S., Appleford, N. E. J., Lange, T., Huttly, A. K., Gaskin, P., Graebe, J. E. and Hedden, P. 1995. Isolation and Expression of Three Gibberellin 20-Oxidase cDNA Clones from Arabidopsis. Plant Physiology. 108 (3), pp. 1049-1057. https://doi.org/10.1104/pp.108.3.1049

Transient expression of CAT in wheat protoplasts.

G - Articles in popular magazines and other technical publications

Jones, M. G. K., De Both, M. T. J., Wu, Q. S., Sparks, C. A., Maddock, S. E. and Gleddie, S. 1986. Transient expression of CAT in wheat protoplasts.