RNAi as an emerging approach to control Fusarium Head Blight disease and mycotoxin contamination in cereals

A - Papers appearing in refereed journals

Machado, A., Brown, N. A., Urban, M., Kanyuka, K. and Hammond-Kosack, K. E. 2017. RNAi as an emerging approach to control Fusarium Head Blight disease and mycotoxin contamination in cereals. Pest Management Science. 74 (4), pp. 790-799.

AuthorsMachado, A., Brown, N. A., Urban, M., Kanyuka, K. and Hammond-Kosack, K. E.
Abstract

Fusarium graminearum is a major fungal pathogen of cereals worldwide, causing seedling, stem base and floral diseases, including Fusarium head blight (FHB). In addition to yield and quality losses, FHB contaminates cereal grain with mycotoxins, including deoxynivalenol, which are harmful to human, animal and ecosystem health. Currently, FHB control is only partially effective due to several intractable problems. RNA interference (RNAi) is a natural mechanism that regulates gene expression. RNAi has been exploited in the development of new genomic tools that allow the targeted silencing of genes of interest in many eukaryotes. Host‐induced gene silencing (HIGS) is a transgenic technology used to silence fungal genes in planta during attempted infection and thereby reduces disease levels. HIGS relies on the host plant's ability to produce mobile small interfering RNA molecules, generated from long double‐stranded RNA, which are complementary to targeted fungal genes. These molecules are transferred from the plant to invading fungi via an uncharacterised mechanism, to cause gene silencing. Here, we describe recent advances in RNAi‐mediated control of plant pathogenic fungi, highlighting the key advantages and disadvantages. We then discuss the developments and implications of combining HIGS with other methods of disease control.

Year of Publication2017
JournalPest Management Science
Journal citation74 (4), pp. 790-799
Digital Object Identifier (DOI)doi:10.1002/ps.4748
PubMed ID28967180
PubMed Central IDPMC5873435
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Empresa Brazileira de Pesquisa Agropecuaria (EMBRAPA) - Brazilian Agricultural Research Corporation
BBSRC Newton funding
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
PHI-base National Capability [2017-2019]
Wheat
Bilateral BBSRC-Embrapa: Using disease risk forecasting, NGS and HIGS to explore and control Fusarium Head Blight disease in wheat fields
DFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience
PhytoPath, an infrastructure for hundreds of plant pathogen genomes [2014-2017]
Do G-protein coupled receptors regulate pathogenesis and mycotoxin biosynthesis in filamentous phytopathogenic fungi?
Publisher's version
Accepted author manuscript
Output statusPublished
Publication dates
Online02 Oct 2017
Publication process dates
Accepted22 Sep 2017
PublisherJohn Wiley & Sons, Ltd
Wiley
Copyright licenseCC BY
Grant IDBB/N018095/1
BB/J/00426X/1
BB/P016855/1
BB/N011686/1
02.15.07.003.00.00
ISSN1526-498X

Permalink - https://repository.rothamsted.ac.uk/item/8v547/rnai-as-an-emerging-approach-to-control-fusarium-head-blight-disease-and-mycotoxin-contamination-in-cereals

23 total views
44 total downloads
2 views this month
0 downloads this month
Download files as zip