A - Papers appearing in refereed journals
Karp, A., Risiott, R., Jones, M. G. K. and Bright, S. W. J. 1984. Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants. Plant Cell, Tissue and Organ Culture. 3 (4), pp. 363-373.
|Authors||Karp, A., Risiott, R., Jones, M. G. K. and Bright, S. W. J.|
Plants were regenerated from cultured excised leaf segments of monohaploid (2n=x=12) and diphaloid (2n=2x=24) potato (Solanum tuberosum L.) and a sample has been studied cytologically. In the case of monohaploids, a single leaf regeneration cycle resulted in almost total recovery of doubled monohaploid plants (2n=2x=24), whilst 50% of the plants regenerated from doubled monohaploid leaves had doubled again to the doubled double monohaploid (or homozygous tetraploid, 2n=4x=48) level. Regeneration from dihaploid leaf pieces also gave a good proportion (60%) of doubled genotypes. Very few mixoploids and very few aneuploids were found. These results, together with the general applicability of the method to a large number of potato cultivars, suggest that it can be used as a simple and reliable method of obtaining homozygous tetraploid potatoes.
|Keywords||Potato chromosome doubling; leaf callus; regeneration|
|Year of Publication||1984|
|Journal||Plant Cell, Tissue and Organ Culture|
|Journal citation||3 (4), pp. 363-373|
|Digital Object Identifier (DOI)||doi:10.1007/bf00043089|
|Open access||Published as non-open access|
|Funder||Biotechnology and Biological Sciences Research Council|
|Publication process dates|
|Accepted||16 Jul 1984|
|Copyright license||Publisher copyright|
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