Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants

A - Papers appearing in refereed journals

Karp, A., Risiott, R., Jones, M. G. K. and Bright, S. W. J. 1984. Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants. Plant Cell, Tissue and Organ Culture. 3 (4), pp. 363-373.

AuthorsKarp, A., Risiott, R., Jones, M. G. K. and Bright, S. W. J.
Abstract

Plants were regenerated from cultured excised leaf segments of monohaploid (2n=x=12) and diphaloid (2n=2x=24) potato (Solanum tuberosum L.) and a sample has been studied cytologically. In the case of monohaploids, a single leaf regeneration cycle resulted in almost total recovery of doubled monohaploid plants (2n=2x=24), whilst 50% of the plants regenerated from doubled monohaploid leaves had doubled again to the doubled double monohaploid (or homozygous tetraploid, 2n=4x=48) level. Regeneration from dihaploid leaf pieces also gave a good proportion (60%) of doubled genotypes. Very few mixoploids and very few aneuploids were found. These results, together with the general applicability of the method to a large number of potato cultivars, suggest that it can be used as a simple and reliable method of obtaining homozygous tetraploid potatoes.

KeywordsPotato chromosome doubling; leaf callus; regeneration
Year of Publication1984
JournalPlant Cell, Tissue and Organ Culture
Journal citation3 (4), pp. 363-373
Digital Object Identifier (DOI)doi:10.1007/bf00043089
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Output statusPublished
Publication process dates
Accepted16 Jul 1984
Copyright licensePublisher copyright
ISSN0167-6857
PublisherSpringer

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