Ploidy variation in Solanum brevidens plants regenerated from protoplasts using an improved culture system

A - Papers appearing in refereed journals

Nelson, R. S., Karp, A. and Bright, S. W. J. 1986. Ploidy variation in Solanum brevidens plants regenerated from protoplasts using an improved culture system. Journal of Experimental Botany. 37 (175), pp. 253-261.

AuthorsNelson, R. S., Karp, A. and Bright, S. W. J.
Abstract

Plating efficiency of cultured protoplasts of Solanum brevidens increased to c. 10% after the addition of 5⋅0 mol m−3 glutamine to the culture medium. Growth of protoplast-derived colonies at densities of 20 colonies cm−3 was obtained by lowering the auxin content alter 4–6 d. A sample of 50 protoplast-derived plants was examined for chromosomal variation. Twelve plants were diploid (2n=24), 26 were tetraploid (2n=48) and 12 were aneuploid at the tetraploid level (2n=48±). Tetraploid and aneuploid plants had broader leaves and set fewer or no seeds compared to the diploid regenerants which were similar in gross morphology and seed set to control plants.

KeywordsSolanum brevidens; Protoplasts; Plant regeneration; Variation
Year of Publication1986
JournalJournal of Experimental Botany
Journal citation37 (175), pp. 253-261
Digital Object Identifier (DOI)doi:10.1093/jxb/37.2.253
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN00220957

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