Physical mapping of the B-hordein loci on barley chromosome 5 by in situ hybridization

A - Papers appearing in refereed journals

Clark, M., Karp, A. and Archer, S. 1989. Physical mapping of the B-hordein loci on barley chromosome 5 by in situ hybridization. Genome. 32 (5), pp. 925-929.

AuthorsClark, M., Karp, A. and Archer, S.
Abstract

Physical mapping of the B-hordein genes in barley (Hordeum vulgare cv. Betzes) was achieved by in situ hybridization of a biotin-labelled B-hordein cRNA probe to chromosome spreads. Lines trisomic for chromosome 5 showed up to 3 signals on interphase nuclei, while no more than 2 signals were observed in normal diploids. An enzyme pretreatment, which produced broken cells, increased the frequency of signal detection. A clear signal was observed on 23% of interphase nuclei and 0.025% of metaphase spreads. The B-hordein genes mapped to 46% of the distance from the centromere on the short arm of chromosome 5. These results are discussed in relation to the barley genetic map and to previous reports of in situ hybridization of low-copy and unique-copy genes in plants.Key words: in situ hybridization, Hordeum vulgare, B-hordein genes, genetic map.

Year of Publication1989
JournalGenome
Journal citation32 (5), pp. 925-929
Digital Object Identifier (DOI)doi:10.1139/g89-531
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN08312796

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