Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and s. tuberosum using non-radioactive digoxigenin-labeled DNA probes

A - Papers appearing in refereed journals

Xu, Y-S., Jones, M. G. K., Karp, A. and Pehu, E. 1993. Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and s. tuberosum using non-radioactive digoxigenin-labeled DNA probes. Theoretical and Applied Genetics. 85 (8), pp. 1017-1022.

AuthorsXu, Y-S., Jones, M. G. K., Karp, A. and Pehu, E.
Abstract

Mitochondrial (mt) DNAs of somatic hybrids obtained by electrical and chemical fusion of mesophyll protoplasts of S. brevidens and a dihaploid line of S. tuberosum PDH 40 were analysed by Southern hybridization using the digoxigenin-labelled mtDNA sequences nad5 or orf25. In the Southern analysis of the hybrid mtDNA probed with nad5, most of the 19 hybrids analyzed had an RFLP pattern similar, but not identical, to one of the parents, S. tuberosum, PDH40. Nineteen percent of the hybrids had most of the S. brevidens fragments. Five of the hybrids had an identical RFLP pattern to either one of the parents while another two hybrids had novel RFLP patterns. Similar results were obtained by Southern analysis with orf25. These results clearly show that mtDNA rearrangements had occurred at a high frequency in the somatic hybrids. There were no differences in the frequencies of rearrangements observed between the hybrids regenerated from chemical and electrical fusions.

KeywordsPotato; solanum-brevidens; mitchondrial DNA; Somatic hybrids; nonradioactive labeling; leaf roll virus; lycopersicon-esculentum; asymmetric hybrids; protoplast; Fusion; Plants; Electrofusion; Resistance; gene; Maize; rearrangements
Year of Publication1993
JournalTheoretical and Applied Genetics
Journal citation85 (8), pp. 1017-1022
Digital Object Identifier (DOI)doi:10.1007/BF00215042
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN00405752

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