An Infective Pyridoxyl-derivative of Potato Virus X: PVX-PLP

A - Papers appearing in refereed journals

Pierpoint, W. S. and Carpenter, J. M. 1978. An Infective Pyridoxyl-derivative of Potato Virus X: PVX-PLP. Journal of General Virology. 38 (3), pp. 505-518. https://doi.org/10.1099/0022-1317-38-3-505

AuthorsPierpoint, W. S. and Carpenter, J. M.
Abstract

he reduced initial product (PVX-PLP) of the reaction of pyridoxyl 5′-phosphate (PLP) and particles of potato virus X (PVX) contains about 1 (0.6 to 1.2) molecules of PLP per protein subunit and is infective. Hydrolysates of the protein contain N-ε-pyridoxyl-lysine. PVX-PLP reacts with chlorogenoquinone to 1/4 of the extent of PVX; similarly, PVX which has reacted with chlorogenoquinone (PVX-Q1) binds only 1/3 to 1/5 as much PLP as does PVX. PVX-PLP contains two types of fluorescent subunit which can be separated by electrophoresis in SDS-acrylamide gels: one of these is fluorescent and is not degraded by brief exposure to trypsin, whereas the other is degraded to a smaller form which is also fluorescent. Tryptic digests of the protein from PVX-PLP contain at least two fluorescent peptides. It is argued that PLP reacts with two lysine ε-amino groups of PVX, one of which also reacts with chlorogenoquinone, and the other of which is recognized by trypsin.

Protein isolated from PVX reacts with up to 6 molecules of PLP. The conformation of the subunits in the intact virus apparently makes many ε-amino groups inaccessible to PLP.

Year of Publication1978
JournalJournal of General Virology
Journal citation38 (3), pp. 505-518
Digital Object Identifier (DOI)https://doi.org/10.1099/0022-1317-38-3-505
Open accessPublished as bronze (free) open access
Publisher's version
Output statusPublished
Publication dates
Print01 Mar 1978
Publication process dates
Accepted30 Sep 1977
Copyright licensePublisher copyright
PublisherMicrobiology Society
ISSN0022-1317

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