A - Papers appearing in refereed journals
Li, F., Ma, K., Chen, X., Zhou, J-J. and Gao, X. 2019. The regulation of three new members of the cytochrome P450 CYP6 family and their promoters in the cotton aphid Aphis gossypii by plant allelochemicals. Pest Management Science. 75 (1), pp. 152-159.
|Authors||Li, F., Ma, K., Chen, X., Zhou, J-J. and Gao, X.|
BACKGROUND: The expression of P450 genes in insects can be induced by plant allelochemicals. To understand the induction mechanisms, we measured the expression proﬁles of three P450 genes and their promoter activities under the induction of plant allelochemicals.
RESULTS: The inducible expression of CYP6CY19 was the highest among three genes, followed by those of CYP6CY22 and CYP6DA1. The regions from −687 to +586bp of CYP6DA1, from−666 to +140bp of CYP6CY19 and from −530 to +218bp of CYP6CY22 were essential for basal transcriptional activity. The cis-elements for plant allelochemicals induction were identiﬁed between −193 and +56bp of CYP6DA1, between −157 and +140bp of CYP6CY19 and between −108 and +218bp of CYP6CY22. These promoter regions were found to contain a potential arylhydrocarbon receptor element binding site with a conservative sequence motif 5′-C/TAC/ANCA/CA-3′. All these four plant allelochemicals were able to induce the expression of these P450 genes. Tannic acid had a better inductive eﬀect than other three plant allelochemicals.
CONCLUSIONS: Our study identiﬁed the plant allelochemical responsive cis-elements. This provides further research targets aimed at understanding the regulatory mechanisms of P450 genes expression and their interactions with plant allelochemicals in insect pests.
|Keywords||Aphisgossypii; CYP6DA1; CYP6CY19; CYP6CY22; Plant allelochemicals; Responsive cis-element|
|Year of Publication||2019|
|Journal||Pest Management Science|
|Journal citation||75 (1), pp. 152-159|
|Digital Object Identifier (DOI)||doi:10.1002/ps.5081|
|Open access||Published as green open access|
|Funder||National Natural Science Foundation of China|
|Biotechnology and Biological Sciences Research Council|
|Funder project or code||31330064|
|Online||23 May 2018|
|Publication process dates|
|Accepted||23 May 2018|
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