The regulation of three new members of the cytochrome P450 CYP6 family and their promoters in the cotton aphid Aphis gossypii by plant allelochemicals

A - Papers appearing in refereed journals

Li, F., Ma, K., Chen, X., Zhou, J-J. and Gao, X. 2019. The regulation of three new members of the cytochrome P450 CYP6 family and their promoters in the cotton aphid Aphis gossypii by plant allelochemicals. Pest Management Science. 75 (1), pp. 152-159.

AuthorsLi, F., Ma, K., Chen, X., Zhou, J-J. and Gao, X.
Abstract

BACKGROUND: The expression of P450 genes in insects can be induced by plant allelochemicals. To understand the induction mechanisms, we measured the expression profiles of three P450 genes and their promoter activities under the induction of plant allelochemicals.

RESULTS: The inducible expression of CYP6CY19 was the highest among three genes, followed by those of CYP6CY22 and CYP6DA1. The regions from −687 to +586bp of CYP6DA1, from−666 to +140bp of CYP6CY19 and from −530 to +218bp of CYP6CY22 were essential for basal transcriptional activity. The cis-elements for plant allelochemicals induction were identified between −193 and +56bp of CYP6DA1, between −157 and +140bp of CYP6CY19 and between −108 and +218bp of CYP6CY22. These promoter regions were found to contain a potential arylhydrocarbon receptor element binding site with a conservative sequence motif 5′-C/TAC/ANCA/CA-3′. All these four plant allelochemicals were able to induce the expression of these P450 genes. Tannic acid had a better inductive effect than other three plant allelochemicals.

CONCLUSIONS: Our study identified the plant allelochemical responsive cis-elements. This provides further research targets aimed at understanding the regulatory mechanisms of P450 genes expression and their interactions with plant allelochemicals in insect pests.

KeywordsAphisgossypii; CYP6DA1; CYP6CY19; CYP6CY22; Plant allelochemicals; Responsive cis-element
Year of Publication2019
JournalPest Management Science
Journal citation75 (1), pp. 152-159
Digital Object Identifier (DOI)doi:10.1002/ps.5081
Open accessPublished as green open access
FunderNational Natural Science Foundation of China
Biotechnology and Biological Sciences Research Council
Funder project or code31330064
Publisher's version
Output statusPublished
Publication dates
Online23 May 2018
Publication process dates
Accepted23 May 2018
PublisherWiley
ISSN1526-498X

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