Metabolomic-Guided Isolation of Bioactive Natural Products from Curvularia sp., an Endophytic Fungus of Terminalia laxiflora

A - Papers appearing in refereed journals

Tawfike, A. F., Abbott, G., Young, L. and Edrada-Ebel, R. 2018. Metabolomic-Guided Isolation of Bioactive Natural Products from Curvularia sp., an Endophytic Fungus of Terminalia laxiflora. Planta Medica. 84 (3), pp. 182-190. https://doi.org/10.1055/s-0043-118807

AuthorsTawfike, A. F., Abbott, G., Young, L. and Edrada-Ebel, R.
Abstract

Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology. Metabolomic tools were successfully employed to compare the metabolite fingerprints of solid and liquid culture extracts of endophyte Curvularia sp. isolated from the leaves of Terminalia laxiflora. Natural product databases were used to dereplicate metabolites in order to determine known compounds and the presence of new natural products. Multivariate analysis highlighted the putative metabolites responsible for the bioactivity of the fungal extract and its fractions on NF-κB and the myelogenous leukemia cell line K562. Metabolomic tools and dereplication studies using high-resolution electrospray ionization mass spectrometry directed the fractionation and isolation of the bioactive components from the fungal extracts. This resulted in the isolation of N-acetylphenylalanine (1) and two linear peptide congeners of 1: dipeptide N-acetylphenylalanyl-L-phenylalanine (2) and tripeptide N-acetylphenylalanyl-L-phenylalanyl-L-leucine (3).

KeywordsMetabolomics ; Dereplication; Endophytic fungi ; Curvularia; Terminalia laxiflora ; Combretaceae
Year of Publication2018
JournalPlanta Medica
Journal citation84 (3), pp. 182-190
Digital Object Identifier (DOI)https://doi.org/10.1055/s-0043-118807
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Accepted author manuscript
Output statusPublished
Publication dates
Online28 Aug 2017
Publication process dates
Accepted21 Aug 2017
Copyright licenseCC BY
PublisherGeorg Thieme Verlag Kg
ISSN0032-0943

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