A - Papers appearing in refereed journals
Tawfike, A. F., Abbott, G., Young, L. and Edrada-Ebel, R. 2018. Metabolomic-Guided Isolation of Bioactive Natural Products from Curvularia sp., an Endophytic Fungus of Terminalia laxiflora. Planta Medica. 84 (3), pp. 182-190. https://doi.org/10.1055/s-0043-118807
Authors | Tawfike, A. F., Abbott, G., Young, L. and Edrada-Ebel, R. |
---|---|
Abstract | Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology. Metabolomic tools were successfully employed to compare the metabolite fingerprints of solid and liquid culture extracts of endophyte Curvularia sp. isolated from the leaves of Terminalia laxiflora. Natural product databases were used to dereplicate metabolites in order to determine known compounds and the presence of new natural products. Multivariate analysis highlighted the putative metabolites responsible for the bioactivity of the fungal extract and its fractions on NF-κB and the myelogenous leukemia cell line K562. Metabolomic tools and dereplication studies using high-resolution electrospray ionization mass spectrometry directed the fractionation and isolation of the bioactive components from the fungal extracts. This resulted in the isolation of N-acetylphenylalanine (1) and two linear peptide congeners of 1: dipeptide N-acetylphenylalanyl-L-phenylalanine (2) and tripeptide N-acetylphenylalanyl-L-phenylalanyl-L-leucine (3). |
Keywords | Metabolomics ; Dereplication; Endophytic fungi ; Curvularia; Terminalia laxiflora ; Combretaceae |
Year of Publication | 2018 |
Journal | Planta Medica |
Journal citation | 84 (3), pp. 182-190 |
Digital Object Identifier (DOI) | https://doi.org/10.1055/s-0043-118807 |
Open access | Published as green open access |
Funder | Biotechnology and Biological Sciences Research Council |
Accepted author manuscript | |
Output status | Published |
Publication dates | |
Online | 28 Aug 2017 |
Publication process dates | |
Accepted | 21 Aug 2017 |
Copyright license | CC BY |
Publisher | Georg Thieme Verlag Kg |
ISSN | 0032-0943 |
Permalink - https://repository.rothamsted.ac.uk/item/8w905/metabolomic-guided-isolation-of-bioactive-natural-products-from-curvularia-sp-an-endophytic-fungus-of-terminalia-laxiflora