RNA interference-mediated knockdown of voltage-gated sodium channel (MpNav) gene causes mortality in peach-potato aphid, Myzus persicae

A - Papers appearing in refereed journals

Tariq, K., Ali, A., Davies, T. G. E., Naz, E., Naz, L., Sohail, S., Hou, M. and Ullah, F. 2019. RNA interference-mediated knockdown of voltage-gated sodium channel (MpNav) gene causes mortality in peach-potato aphid, Myzus persicae. Scientific Reports. 9 (1), p. 5291.

AuthorsTariq, K., Ali, A., Davies, T. G. E., Naz, E., Naz, L., Sohail, S., Hou, M. and Ullah, F.
Abstract

Voltage-gated sodium channels (VGSC) are transmembrane proteins that generate an action potential in excitable cells and play an essential role in neuronal signaling. Since VGSCs play a crucial role in nerve transmission they have become primary targets for a broad range of commercial insecticides. RNA interference (RNAi) is a valuable reverse genetics tool used in functional genomics, but recently, it has also shown promise as a novel agent that could be used to control agricultural insect pests. In this study, we targeted the VGSC (MpNav) gene in the peach-potato aphid Myzus persicae, by oral feeding of artificial diets mixed with dsRNAs. Knock-down of MpNav gene expression caused up to 65% mortality in 3rd instar nymphs. Moreover, significantly lower fecundity and longevity was observed in adult aphids that had been fed with dsMpNav solution at the nymphal stage. Analysis of gene expression by qRT-PCR indicated that the aphid mortality rates and the lowered fecundity and longevity were attributable to the down-regulation of MpNav by RNAi. Taken together, our results show that MpNav is a viable candidate target gene for the development of an RNAi-based bio-aphicide.

KeywordsVoltage-gated sodium channel; RNAi; Aphid
Year of Publication2019
JournalScientific Reports
Journal citation9 (1), p. 5291
Digital Object Identifier (DOI)doi:10.1038/s41598-019-41832-8
PubMed ID30923355
PubMed Central IDPMC6439219
Open accessPublished as ‘gold’ (paid) open access
Funder project or codeBBSRC Strategic Programme in Smart Crop Protection
FunderBBSRC Industrial Strategy Challenge
Publisher's versions41598-019-41832-8.pdf
Output statusPublished
Publication dates
Online28 Mar 2019
Publication process dates
Accepted19 Mar 2019
Copyright licenseCC BY
PublisherNature Publishing Group
ISSN2045-2322

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