Effects of polyphenol oxidase on lipolysis and proteolysis of red clover silage with and without a silage inoculant (Lactobacillus plantarum L54)

A - Papers appearing in refereed journals

Lee, M. R. F., Scott, M. B., Tweed, J. K. S., Minchin, F. R. and Davies, D. R. 2008. Effects of polyphenol oxidase on lipolysis and proteolysis of red clover silage with and without a silage inoculant (Lactobacillus plantarum L54). Animal Feed Science and Technology. 144 (1-2), pp. 125-136.

AuthorsLee, M. R. F., Scott, M. B., Tweed, J. K. S., Minchin, F. R. and Davies, D. R.
Abstract

Polyphenol oxidase (PPO) has been shown to reduce proteolysis and lipolysis in red clover through deactivation of proteolytic and lipolytic enzymes and/or through formation of protein-phenol-lipid complexes. This experiment investigated the time course of both lipolysis and proteolysis in two red clover lines with different PPO activities with and without addition of a silage inoculant to help understand the action of PPO in the silo, and its potential effects on protein and glycerolbased lipid conservation, and to determine effects of a more rapid pH reduction with inoculation on PPO activity. Four silages were prepared from high or low PPO precision chopped red clover in 60 test-tube-silos, each containing 120 g fresh weight: (a) high PPO red clover without inoculation (H-),(b) low PPO red clover without inoculation (L-), (c) high PPO red clover with inoculation (H+), and (d) low PPO red clover with inoculation (L+). Each treatment had three replicates for each time point of 1, 2, 4, 8 and 90 days. The inoculant used was Lactobacillus plantarum strain L54 applied at a rate of 10(6) CFU/g fresh weight. Silage pH was reduced (P <0.00 1) by inoculation with no effect of PPO. Inoculation had no effect on either lipolysis or free amino acid release, although more (P <0.01) soluble protein and less (P <0.01) ammonia-N was in inoculated silages. H silages had a lower level of both proteolysis (release of free amino acids, P <0.05) and lipolysis (loss of membrane lipid, P <0.01) than L red clover silages. Results indicate that PPO reduced proteolysis and lipolysis in the silo and that inoculation had no adverse effects on PPO activity

Year of Publication2008
JournalAnimal Feed Science and Technology
Journal citation144 (1-2), pp. 125-136
Digital Object Identifier (DOI)doi:10.1016/j.anifeedsci.2007.09.035
Open accessPublished as non-open access
ISSN0377-8401
PublisherElsevier Science Bv

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