A - Papers appearing in refereed journals
Hirsch, P. R. and Beggs, J. D. 1984. Agrobacterium tumefaciens T-DNA in the yeast Saccharomyces cerevisiae. Molecular and General Genetics (MGG). 195 (1-2), pp. 209-214.
|Authors||Hirsch, P. R. and Beggs, J. D.|
A section of the octopine Ti plasmid pTiACH5 including the region always integrated in crown gall tumour cells (the TL-DNA) was cloned into the yeast — E. coli shuttle vector pJDB207. This plasmid, pOTY8, was transformed into S. cerevisiae where transcription of the T-DNA was demonstrated, but there was no evidence for the production of octopine synthetase.
The TL-DNA was cloned into a derivative of pJDB207 unable to replicate in yeast, but the TL-DNA did not appear to contain any autonomously replicating sequences (ars) functional in yeast.
|Keywords||Tumour cell; Gall; Saccharomyces Cerevisiae; Saccharomyces; Agrobacterium|
|Year of Publication||1984|
|Journal||Molecular and General Genetics (MGG)|
|Journal citation||195 (1-2), pp. 209-214|
|Digital Object Identifier (DOI)||doi:10.1007/BF00332748|
|Open access||Published as non-open access|
|01 Jun 1984|
|Copyright license||Publisher copyright|
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