Agrobacterium tumefaciens T-DNA in the yeast Saccharomyces cerevisiae

A - Papers appearing in refereed journals

Hirsch, P. R. and Beggs, J. D. 1984. Agrobacterium tumefaciens T-DNA in the yeast Saccharomyces cerevisiae. Molecular and General Genetics (MGG). 195 (1-2), pp. 209-214.

AuthorsHirsch, P. R. and Beggs, J. D.
Abstract

A section of the octopine Ti plasmid pTiACH5 including the region always integrated in crown gall tumour cells (the TL-DNA) was cloned into the yeast — E. coli shuttle vector pJDB207. This plasmid, pOTY8, was transformed into S. cerevisiae where transcription of the T-DNA was demonstrated, but there was no evidence for the production of octopine synthetase.

The TL-DNA was cloned into a derivative of pJDB207 unable to replicate in yeast, but the TL-DNA did not appear to contain any autonomously replicating sequences (ars) functional in yeast.

KeywordsTumour cell; Gall; Saccharomyces Cerevisiae; Saccharomyces; Agrobacterium
Year of Publication1984
JournalMolecular and General Genetics (MGG)
Journal citation195 (1-2), pp. 209-214
Digital Object Identifier (DOI)doi:10.1007/BF00332748
Open accessPublished as non-open access
Output statusPublished
Publication dates
Print01 Jun 1984
Copyright licensePublisher copyright

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