Identification of a major QTL and associated marker for high arabinoxylan fibre in white wheat flour

A - Papers appearing in refereed journals

Lovegrove, A., Wingen, L. U., Plummer, A., Wood, A., Passmore, D., Kosik, O., Freeman, J., Mitchell, R. A. C., Ulker, M., Tremmel-Bede, K., Rakszegi, M., Bedő, Z., Petterant, M.-R., Charmet, G., Leverington Waite, M., Orford, S., Burridge, A., Pellny, T. K., Shewry, P. R. and Griffiths, S. 2019. Identification of a major QTL and associated marker for high arabinoxylan fibre in white wheat flour. Plant Biotechnology Journal.

AuthorsLovegrove, A., Wingen, L. U., Plummer, A., Wood, A., Passmore, D., Kosik, O., Freeman, J., Mitchell, R. A. C., Ulker, M., Tremmel-Bede, K., Rakszegi, M., Bedő, Z., Petterant, M.-R., Charmet, G., Leverington Waite, M., Orford, S., Burridge, A., Pellny, T. K., Shewry, P. R. and Griffiths, S.
Abstract

Dietary fibre (DF) has multiple health benefits, and wheat products are major sources of DF for human health. However, DF is depleted in white flour, which is most widely consumed, compared to wholegrain. The major type of DF in white wheat flour is the cell wall polysaccharide arabinoxylan (AX). Previous studies have identified the Chinese wheat cultivar Yumai 34 as having unusually high contents of AX in both water-soluble and insoluble forms. We have therefore used populations generated from crosses between Yumai 34 and four other wheat cultivars, three with average contents of AX (Ukrainka, Altigo and Claire) and one also having unusually high AX (Valoris), in order to map QTLs for soluble AX (determined as relative viscosity) of aqueous extracts of wholemeal flours) and total AX (determined by enzyme fingerprinting of white flour). A number of QTL were mapped, but most were only detected in one or two crosses. However, all four crosses showed strong QTLs for high RV/total AX on chromosome 1B, with Yumai 34 being the increasing parent, and a KASP marker for the high AX Yumai 34 allele was validated by analysis of high AX lines derived from Yumai 34 but selected by biochemical analysis. A QTL for RV was mapped on chromosome 6B in Yumai 34 x Valoris, with Valoris being the increasing allele, which was consistent with the observation of transgressive segregation for this trait. The data indicate that breeding can be used to develop wheat with high AX fibre in white flour.

KeywordsWheat; White flour; Dietary fibre; Arabinoxylan; Relative viscosity; QTL
Year of Publication2019
JournalPlant Biotechnology Journal
Digital Object Identifier (DOI)doi:10.1101/705343
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience
High Fibre Wheat for Healthier White Bread
Publisher's version
Output statusSubmitted
Copyright licenseAuthor copyright
PublisherWiley
ISSN1467-7644

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