A - Papers appearing in refereed journals
Jones, F. P., Clark, I. M., King, R., Woodward, M. J. and Hirsch, P. R. 2016. Novel European free-living, nondiazotrophic Bradyrhizobium isolates from contrasting soils that lack nodulation and nitrogen fixation genes – a genome comparison. Scientific Reports. 6 (25858), p. 10p.
|Authors||Jones, F. P., Clark, I. M., King, R., Woodward, M. J. and Hirsch, P. R.|
The slow-growing genus Bradyrhizobium is biologically important in soils, with different representatives found to perform a range of biochemical functions including photosynthesis, induction of root nodules and symbiotic nitrogen fixation and denitrification. Consequently, the role of the genus in soil ecology and biogeochemical transformations is of agricultural and environmental significance. Some isolates of Bradyrhizobium have been shown to be non-symbiotic and do not possess the ability to form nodules. Here we present the genome and gene annotations of two such free-living Bradyrhizobium isolates, named G22 and BF49, from soils with differing long-term management regimes (grassland and bare fallow respectively) in addition to carbon metabolism analysis. These Bradyrhizobium isolates are the first to be isolated and sequenced from European soil and are the first free-living Bradyrhizobium isolates, lacking both nodulation and nitrogen fixation genes, to have their genomes sequenced and assembled from cultured samples. The G22 and BF49 genomes are distinctly different with respect to size and number of genes; the grassland isolate also contains a plasmid. There are also a number of functional differences between these isolates and other published genomes, suggesting that this ubiquitous genus is extremely heterogeneous and has roles within the community not including symbiotic nitrogen fixation.
|Year of Publication||2016|
|Journal citation||6 (25858), p. 10p|
|Digital Object Identifier (DOI)||doi:10.1038/srep25858|
|Open access||Published as ‘gold’ (paid) open access|
|Funder||Biotechnology and Biological Sciences Research Council|
|Lawes Agricultural Trust|
|Funder project or code||Optimisation of nutrients in soil-plant systems: How can we control nitrogen cycling in soil?|
|Online||10 May 2016|
|Publication process dates|
|Accepted||25 Apr 2016|
|Publisher||Nature Publishing Group|
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