High level accumulation of EPA and DHA in field-grown transgenic Camelina – a multi-territory evaluation of TAG accumulation and heterogeneity

A - Papers appearing in refereed journals

Han, L., Usher, S. L., Sandgrind, S., Hassall, K. L., Sayanova, O. V., Michaelson, L. V., Haslam, R. P. and Napier, J. A. 2020. High level accumulation of EPA and DHA in field-grown transgenic Camelina – a multi-territory evaluation of TAG accumulation and heterogeneity . Plant Biotechnology Journal. https://doi.org/10.1111/pbi.13385

AuthorsHan, L., Usher, S. L., Sandgrind, S., Hassall, K. L., Sayanova, O. V., Michaelson, L. V., Haslam, R. P. and Napier, J. A.
Abstract

The transgene-directed accumulation of non-native omega-3 long chain polyunsaturated fatty acids in the seed oil of Camelina sativa (Camelina) was evaluated in the field, in distinct geographical and regulatory locations. A construct, DHA2015.1, containing an optimal combination of biosynthetic genes, was selected for experimental field release in the UK, USA and Canada, and the accumulation of eicosapentaenoic acid and docosahexaenoic acid determined. The occurrence of these fatty acids in different triacylglycerol species was monitored and found to follow a broad trend irrespective of the agricultural environment. This is a clear demonstration of the stability and robust nature of the transgenic trait for omega-3 long chain polyunsaturated fatty acids in Camelina. Examination of nonseed tissues for the unintended accumulation of EPA and DHA failed to identify their presence in leaf, stem, flower, anther or capsule shell material, confirming the seed-specific accumulation of these novel fatty acids. Collectively these data confirm the promise of GM plant-based sources of so called omega-3 fish oils as a sustainable replacement for oceanically-derived oils.

KeywordsOmega-3; Camelina; Metabolomic engineering; GM field trials
Year of Publication2020
JournalPlant Biotechnology Journal
Digital Object Identifier (DOI)https://doi.org/10.1111/pbi.13385
Open accessPublished as bronze (free) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeTailoring Plant Metabolism ISP
Designing Seeds for Nutrition and Health (DS) [ISPG]
Publisher's version
Accepted author manuscript
Output statusPublished
Publication dates
Online18 Apr 2020
Publication process dates
Accepted07 Apr 2020
PublisherWiley
ISSN1467-7644

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