A - Papers appearing in refereed journals
Han, L., Usher, S. L., Sandgrind, S., Hassall, K. L., Sayanova, O. V., Michaelson, L. V., Haslam, R. P. and Napier, J. A. 2020. High level accumulation of EPA and DHA in field-grown transgenic Camelina – a multi-territory evaluation of TAG accumulation and heterogeneity . Plant Biotechnology Journal. 18 (11), pp. 2280-2291. https://doi.org/10.1111/pbi.13385
Authors | Han, L., Usher, S. L., Sandgrind, S., Hassall, K. L., Sayanova, O. V., Michaelson, L. V., Haslam, R. P. and Napier, J. A. |
---|---|
Abstract | The transgene-directed accumulation of non-native omega-3 long chain polyunsaturated fatty acids in the seed oil of Camelina sativa (Camelina) was evaluated in the field, in distinct geographical and regulatory locations. A construct, DHA2015.1, containing an optimal combination of biosynthetic genes, was selected for experimental field release in the UK, USA and Canada, and the accumulation of eicosapentaenoic acid and docosahexaenoic acid determined. The occurrence of these fatty acids in different triacylglycerol species was monitored and found to follow a broad trend irrespective of the agricultural environment. This is a clear demonstration of the stability and robust nature of the transgenic trait for omega-3 long chain polyunsaturated fatty acids in Camelina. Examination of nonseed tissues for the unintended accumulation of EPA and DHA failed to identify their presence in leaf, stem, flower, anther or capsule shell material, confirming the seed-specific accumulation of these novel fatty acids. Collectively these data confirm the promise of GM plant-based sources of so called omega-3 fish oils as a sustainable replacement for oceanically-derived oils. |
Keywords | Omega-3; Camelina; Metabolomic engineering; GM field trials |
Year of Publication | 2020 |
Journal | Plant Biotechnology Journal |
Journal citation | 18 (11), pp. 2280-2291 |
Digital Object Identifier (DOI) | https://doi.org/10.1111/pbi.13385 |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Tailoring Plant Metabolism ISP |
Tailoring Plant Metabolism (TPM) - Work package 1 (WP1) - High value lipids for health and industry | |
Designing Seeds for Nutrition and Health (DS) [ISPG] | |
Publisher's version | |
Accepted author manuscript | |
Output status | Published |
Publication dates | |
Online | 18 Apr 2020 |
Publication process dates | |
Accepted | 07 Apr 2020 |
Publisher | Wiley |
ISSN | 1467-7644 |
Permalink - https://repository.rothamsted.ac.uk/item/97772/-high-level-accumulation-of-epa-and-dha-in-field-grown-transgenic-camelina-a-multi-territory-evaluation-of-tag-accumulation-and-heterogeneity