The Saccharomyces cerevisiae ABCD subfamily transporter Pxa1/Pxa2p co-imports CoASH into the peroxisome

A - Papers appearing in refereed journals

Van Roermund, C. W. T., IJlst, L., Baker, A., Wanders, R. J. A., Theodoulou, F. L. and Waterham, H. R. 2020. The Saccharomyces cerevisiae ABCD subfamily transporter Pxa1/Pxa2p co-imports CoASH into the peroxisome. FEBS Letters. https://doi.org/10.1002/1873-3468.13974

AuthorsVan Roermund, C. W. T., IJlst, L., Baker, A., Wanders, R. J. A., Theodoulou, F. L. and Waterham, H. R.
Abstract

ABC subfamily D transporters are important for the uptake of fatty acids into peroxisomes, where they undergo β-oxidation. Genetic and biochemical evidence indicates that the transporters accept fatty acyl-CoAs but that these are cleaved during the transport cycle and re-esterified in the peroxisomal lumen. However, it is not known whether CoASH is released inside or outside the peroxisome. Here we use Saccharomyces cerevisiae to demonstrate that free CoA is released in the peroxisomal lumen. Thus, ABCD transporters provide an import pathway for CoA. Implications for peroxisomal CoA homeostasis are discussed.

KeywordsABC transporter; Peroxisome; Acyl-CoA; Thioesterase; Coenzyme A; Coenzyme homeostasis
Year of Publication2020
JournalFEBS Letters
Digital Object Identifier (DOI)https://doi.org/10.1002/1873-3468.13974
Web address (URL)https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13974
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCleavage of acyl-CoA by peroxisomal ABC subfamily D transporters in peroxisomes: mechanism and functional roles
Output statusSubmitted
Publication dates
Online28 Oct 2020
Publication process dates
Accepted15 Oct 2020
PublisherFEBS Press
ISSN0014-5793

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