Fly-Tox: A panel of transgenic flies expressing pest and pollinator cytochrome P450s

A - Papers appearing in refereed journals

McLeman, A., Troczka, B. J., Homem, R. A., Duarte, A., Zimmer, C. T., Garrood, W. T., Pym, A., Beadle, K., Reid, R. J., Douris, V., Vontas, J., Davies, T. G. E., Ffrench-Constant, R., Nauen, R. and Bass, C. G. 2020. Fly-Tox: A panel of transgenic flies expressing pest and pollinator cytochrome P450s. Pesticide Biochemistry and Physiology. p. 104674. https://doi.org/10.1016/j.pestbp.2020.104674

AuthorsMcLeman, A., Troczka, B. J., Homem, R. A., Duarte, A., Zimmer, C. T., Garrood, W. T., Pym, A., Beadle, K., Reid, R. J., Douris, V., Vontas, J., Davies, T. G. E., Ffrench-Constant, R., Nauen, R. and Bass, C. G.
Abstract

There is an on-going need to develop new insecticides that are not compromised by resistance and that have improved environmental profiles. However, the cost of developing novel compounds has increased significantly over the last two decades. This is in part due to increased regulatory requirements, including the need to screen both pest and pollinator insect species to ensure that pre-existing resistance will not hamper the efficacy of a new insecticide via cross-resistance, or adversely affect non-target insect species. To add to this problem the collection and maintenance of toxicologically relevant pest and pollinator species and strains is costly and often difficult. Here we present Fly-Tox, a panel of publicly available transgenic Drosophila melanogaster lines each containing one or more pest or pollinator P450 genes that have been previously shown to metabolise insecticides. We describe the range of ways these tools can be used, including in predictive screens to avoid pre-existing cross-resistance, to identify potential resistance-breaking inhibitors, in the initial assessment of potential insecticide toxicity to bee pollinators, and identifying harmful pesticide-pesticide interactions.

KeywordsDrosophila; Transgenic; Cytochrome P450; Toxicology; Insecticide
Year of Publication2020
JournalPesticide Biochemistry and Physiology
Journal citationp. 104674
Digital Object Identifier (DOI)https://doi.org/10.1016/j.pestbp.2020.104674
Web address (URL)https://www.sciencedirect.com/science/article/pii/S0048357520301693?via%3Dihub
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
European Research Council
Funder project or codeBBSRC Strategic Programme in Smart Crop Protection
15076182
European Union’s Horizon 2020 research and innovation programme
Publisher's version
Copyright license
CC BY 4.0
Output statusPublished
Publication dates
Online06 Aug 2020
Publication process dates
Accepted31 Jul 2020
PublisherElsevier
ISSN0048-3575

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