Suppression of a BAHD acyltransferase decreases p-coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis

A - Papers appearing in refereed journals

Mota, T. R., De Souza, W. R., Oliveira, D. M., Martins, P. K., Sampaio, B. L., Vinecky, F., Ribeiro, A. P., Duarte, K. E., Pacheco, T. F., De K. V. Monteiro, N., Campanha, R. B., Marchiosi, R., Vieriea, D. S., Kobayashi, A. K., De O Molinari, P. A., Ferrarese-Filho, O., Mitchell, R. A. C., Molinari, H. B. and Dos Santos, W. D. 2020. Suppression of a BAHD acyltransferase decreases p-coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis. Plant Journal. p. 15046. https://doi.org/10.1111/tpj.15046

AuthorsMota, T. R., De Souza, W. R., Oliveira, D. M., Martins, P. K., Sampaio, B. L., Vinecky, F., Ribeiro, A. P., Duarte, K. E., Pacheco, T. F., De K. V. Monteiro, N., Campanha, R. B., Marchiosi, R., Vieriea, D. S., Kobayashi, A. K., De O Molinari, P. A., Ferrarese-Filho, O., Mitchell, R. A. C., Molinari, H. B. and Dos Santos, W. D.
Abstract

Grass cell walls have hydroxycinnamic acids attached to arabinosyl residues of arabinoxylan (AX), and certain BAHD acyltransferases are involved in their addition. In this study, we characterized one of these BAHD genes in the cell wall of the model grass Setaria viridis. RNAi silenced lines of S. viridis (SvBAHD05) presented a decrease of up to 42% of ester-linked p-coumarate (pCA) and 50% of pCA-arabinofuranosyl, across three generations. Biomass from SvBAHD05 silenced plants exhibited up to 32% increase in biomass saccharification after acid pre-treatment, with no change in total lignin. Molecular dynamics simulations suggested that SvBAHD05 is a p-coumaroyl coenzyme A transferase (PAT) mainly involved in the addition of pCA to the arabinofuranosyl residues of AX in Setaria. Thus, our results provide evidence of p-coumaroylation of AX promoted by SvBAHD05 acyltransferase in the cell wall of the model grass S. viridis. Furthermore, SvBAHD05 is a promising biotechnological target to engineer crops for improved biomass digestibility for biofuels, biorefineries and animal feeding.

Year of Publication2020
JournalPlant Journal
Journal citationp. 15046
Digital Object Identifier (DOI)https://doi.org/10.1111/tpj.15046
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Output statusPublished
Publication dates
Online28 Oct 2020
Publication process dates
Accepted23 Sep 2020
PublisherWiley

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