The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis

A - Papers appearing in refereed journals

Verhertbruggen, Y., Bouder, A., Vigouroux, J., Alvarado, C., Geairon, A., Guillon, F., Wilkinson, M. D., Stritt, F., Pauly, M., Lee, M. Y., Mortimer, J. C., Scheller, H. V., Mitchell, R. A. C., Voiniciuc, C., Saulnier, L. and Chateigner-Boutin, A.-L.. 2020. The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis . Plant Science. 302, p. 110693. https://doi.org/10.1016/j.plantsci.2020.110693

AuthorsVerhertbruggen, Y., Bouder, A., Vigouroux, J., Alvarado, C., Geairon, A., Guillon, F., Wilkinson, M. D., Stritt, F., Pauly, M., Lee, M. Y., Mortimer, J. C., Scheller, H. V., Mitchell, R. A. C., Voiniciuc, C., Saulnier, L. and Chateigner-Boutin, A.-L..
Abstract

Mannan is a class of cell wall polysaccharides widespread in the plant kingdom. Mannan structure and properties vary according to species and organ. The cell walls of cereal grains have been extensively studied due to their role in cereal processing and to their beneficial effect on human health as dietary fiber. Recently, we showed that mannan in wheat (Triticum aestivum) grain endosperm has a linear structure of β-1,4-linked mannose residues. The aim of this work was to study the biosynthesis and function of wheat grain mannan. We showed that mannan is deposited in the endosperm early during grain development, and we identified candidate mannan biosynthetic genes expressed in the endosperm. The functional study in wheat was unsuccessful therefore our best candidate genes were expressed in heterologous systems. The endosperm-specificTaCslA12 gene expressed in Pichia pastoris and in an Arabidopsis thaliana mutant depleted in glucomannan led to the production of wheat-like linear mannan lacking glucose residues and with moderate acetylation. Therefore, this gene encodes a mannan synthase and is likely responsible for the synthesis of wheat endosperm mannan.

KeywordsWheat grain; Developing endosperm; Cell walls; Mannan; Heterologous expression
Year of Publication2020
JournalPlant Science
Journal citation302, p. 110693
Digital Object Identifier (DOI)https://doi.org/10.1016/j.plantsci.2020.110693
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat WP2.2
Output statusPublished
Publication dates
Online03 Oct 2020
Publication process dates
Accepted26 Sep 2020
PublisherElsevier Ireland Ltd
ISSN0168-9452

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