Contrasting Responses of Rhizosphere Bacterial, Fungal, Protist and Nematode Communities to Nitrogen Fertilisation and Crop Genotype in Field Grown Oilseed Rape (Brassica napus)

A - Papers appearing in refereed journals

Picot, E., Hale, C. C., Hilton, S., Teakle, G. R., Schafer, H., Huang, Y-J., Perryman, S. A. M., West, J. S. and Bending, G. D. 2021. Contrasting Responses of Rhizosphere Bacterial, Fungal, Protist and Nematode Communities to Nitrogen Fertilisation and Crop Genotype in Field Grown Oilseed Rape (Brassica napus). Frontiers in Sustainable Food Systems. 5 (article), p. 613269. https://doi.org/10.3389/fsufs.2021.613269

AuthorsPicot, E., Hale, C. C., Hilton, S., Teakle, G. R., Schafer, H., Huang, Y-J., Perryman, S. A. M., West, J. S. and Bending, G. D.
Abstract

The rhizosphere microbiome is considered to play a key role in determining crop health. However, current understanding of the factors which shape assembly and composition of the microbiome is heavily biased toward bacterial communities, and the relevance for other microbial groups is unclear. Furthermore, community assembly is determined by a variety of factors, including host genotype, environment and agricultural management practices, and their relative importance and interactions remain to be elucidated. We investigated the impact of nitrogen fertilization on rhizosphere bacterial, fungal, nematode and protist communities of 10 contrasting oilseed rape genotypes in a field experiment. We found significant differences in the composition of bacteria, fungi, protist and nematode communities between the rhizosphere and bulk soil. Nitrogen application had a significant but weak effect on fungal, bacterial, and protist community composition, and this was associated with increased relative abundance of a complex of fungal pathogens in the rhizosphere and soil, including Mycosphaerella sp. and Leptosphaeria sp. Network analysis showed that nitrogen application had different effects on microbial community connectivity in the soil and rhizosphere. Crop genotype significantly affected fungal community composition, with evidence for a degree of genotype specificity for a number of pathogens, including L. maculans, Alternaria sp., Pyrenopeziza brassicae, Olpidium brassicae, and L. biglobosa, and also potentially beneficial Heliotales root endophytes. Crop genotype had no significant effect on assembly of bacteria, protist or nematode communities. There was no relationship between genetic distance of crop genotypes and the extent of dissimilarity of rhizosphere microbial communities. Field disease assessment confirmed infection of crops by Leptosphaeria sp., P. brassicae, and Alternaria sp., indicating that rhizosphere microbiome sequencing was an effective indicator of plant health. We conclude that under field conditions soil and rhizosphere nutrient stoichiometry and crop genotype are key factors determining crop health by influencing the infection of roots by pathogenic and mutualistic fungal communities, and the connectivity and stability of rhizosphere microbiome interaction networks

KeywordsInteraction networks; Pathogens; Fungi; Nematode; Protist; Nitrogen; Crop genotype; Rhizosphere
Year of Publication2021
JournalFrontiers in Sustainable Food Systems
Journal citation5 (article), p. 613269
Digital Object Identifier (DOI)https://doi.org/10.3389/fsufs.2021.613269
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Department of Environment, Food and Rural Affairs
Funder project or code BB/J019690/1
BB/L025892/1
IF0144
Publisher's version
Output statusPublished
Publication dates
Online08 Apr 2021
Publication process dates
Accepted04 Mar 2021
PublisherFrontiers Media SA
ISSN2571-581X

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