Synaptotagmins at the endoplasmic reticulum–plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress

A - Papers appearing in refereed journals

Ruiz-Lopez, N., Perez-Sancho, J., Del Valle, A. E., Haslam, R. P., Vanneste, S., Catala, R., Perea-Resa, C., Van Damme, D., Garcia-Hernandez, S., Albert A., Vallarino, J., Lin, J., Friml, J., Macho, A. P., Salinas, J., Rosado, A., Napier, J. A., Amorim-Silva, V. and Botella, M. A. 2021. Synaptotagmins at the endoplasmic reticulum–plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. The Plant Cell. p. koab122. https://doi.org/10.1093/plcell/koab122

AuthorsRuiz-Lopez, N., Perez-Sancho, J., Del Valle, A. E., Haslam, R. P., Vanneste, S., Catala, R., Perea-Resa, C., Van Damme, D., Garcia-Hernandez, S., Albert A., Vallarino, J., Lin, J., Friml, J., Macho, A. P., Salinas, J., Rosado, A., Napier, J. A., Amorim-Silva, V. and Botella, M. A.
Abstract

Endoplasmic reticulum-plasma membrane contact sites (ER-PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER-PM protein tether synaptotagmin1 (SYT1) exhibit decreased plasma membrane (PM) integrity under multiple abiotic stresses such as freezing, high salt, osmotic stress and mechanical damage. Here, we show that, together with SYT1, the stress-induced SYT3 is an ER-PM tether that also functions in maintaining PM integrity. The ER-PM CS localization of SYT1 and SYT3 is dependent on PM phosphatidylinositol-4-phosphate and is regulated by abiotic stress. Lipidomic analysis revealed that cold stress increased the accumulation of diacylglycerol at the PM in a syt1/3 double mutant relative to wild type while the levels of most glycerolipid species remain unchanged. Additionally, the SYT1-green fluorescent protein (GFP) fusion preferentially binds diacylglycerol in vivo with little affinity for polar glycerolipids. Our work uncovers a SYT-dependent mechanism of stress adaptation counteracting the detrimental accumulation of diacylglycerol at the PM produced during episodes of abiotic stress.

Year of Publication2021
JournalThe Plant Cell
Journal citationp. koab122
Digital Object Identifier (DOI)https://doi.org/10.1093/plcell/koab122
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeTPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry
Accepted author manuscript
Output statusPublished
Publication dates
Online04 May 2021
Publication process dates
Accepted25 Apr 2021
PublisherAmerican Society of Plant Biologists (ASPB)
ISSN1532298X

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