A - Papers appearing in refereed journals
Ruiz-Lopez, N., Perez-Sancho, J., Del Valle, A. E., Haslam, R. P., Vanneste, S., Catala, R., Perea-Resa, C., Van Damme, D., Garcia-Hernandez, S., Albert A., Vallarino, J., Lin, J., Friml, J., Macho, A. P., Salinas, J., Rosado, A., Napier, J. A., Amorim-Silva, V. and Botella, M. A. 2021. Synaptotagmins at the endoplasmic reticulum–plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. The Plant Cell. 33 (7), p. 2431–2453. https://doi.org/10.1093/plcell/koab122
Authors | Ruiz-Lopez, N., Perez-Sancho, J., Del Valle, A. E., Haslam, R. P., Vanneste, S., Catala, R., Perea-Resa, C., Van Damme, D., Garcia-Hernandez, S., Albert A., Vallarino, J., Lin, J., Friml, J., Macho, A. P., Salinas, J., Rosado, A., Napier, J. A., Amorim-Silva, V. and Botella, M. A. |
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Abstract | Endoplasmic reticulum-plasma membrane contact sites (ER-PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER-PM protein tether synaptotagmin1 (SYT1) exhibit decreased plasma membrane (PM) integrity under multiple abiotic stresses such as freezing, high salt, osmotic stress and mechanical damage. Here, we show that, together with SYT1, the stress-induced SYT3 is an ER-PM tether that also functions in maintaining PM integrity. The ER-PM CS localization of SYT1 and SYT3 is dependent on PM phosphatidylinositol-4-phosphate and is regulated by abiotic stress. Lipidomic analysis revealed that cold stress increased the accumulation of diacylglycerol at the PM in a syt1/3 double mutant relative to wild type while the levels of most glycerolipid species remain unchanged. Additionally, the SYT1-green fluorescent protein (GFP) fusion preferentially binds diacylglycerol in vivo with little affinity for polar glycerolipids. Our work uncovers a SYT-dependent mechanism of stress adaptation counteracting the detrimental accumulation of diacylglycerol at the PM produced during episodes of abiotic stress. |
Year of Publication | 2021 |
Journal | The Plant Cell |
Journal citation | 33 (7), p. 2431–2453 |
Digital Object Identifier (DOI) | https://doi.org/10.1093/plcell/koab122 |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Tailoring Plant Metabolism (TPM) - Work package 1 (WP1) - High value lipids for health and industry |
Publisher's version | |
Accepted author manuscript | |
Output status | Published |
Publication dates | |
Online | 04 May 2021 |
Publication process dates | |
Accepted | 25 Apr 2021 |
Publisher | American Society of Plant Biologists (ASPB) |
ISSN | 1532-298X |
Permalink - https://repository.rothamsted.ac.uk/item/98494/synaptotagmins-at-the-endoplasmic-reticulum-plasma-membrane-contact-sites-maintain-diacylglycerol-homeostasis-during-abiotic-stress