Plastidial acyl carrier protein Delta 9-desaturase modulates eicosapentaenoic acid biosynthesis and triacylglycerol accumulation in Phaeodactylum tricornutum

A - Papers appearing in refereed journals

Smith, R., Jouhet, J., Nekrasov, V., Marechal, E., Napier, J. A. and Sayanova, O. V. 2021. Plastidial acyl carrier protein Delta 9-desaturase modulates eicosapentaenoic acid biosynthesis and triacylglycerol accumulation in Phaeodactylum tricornutum. Plant Journal. https://doi.org/10.1111/tpj.15231

AuthorsSmith, R., Jouhet, J., Nekrasov, V., Marechal, E., Napier, J. A. and Sayanova, O. V.
Abstract

The unicellular marine diatom Phaeodactylum tricornutum accumulates up to 35% eicosapentaenoic acid (EPA, 20:5n3) and has been used as a model organism to study long chain polyunsaturated fatty acids (LCPUFA) biosynthesis due to an excellent annotated genome sequence and established transformation system. In P. tricornutum, the majority of EPA accumulates in polar lipids, particularly in galactolipids such as mono- and di-galactosyldiacylglycerol. LC-PUFA biosynthesis is considered to start from oleic acid (18:1n9). EPA can be synthesized via a series of desaturation and elongation steps occurring at the endoplasmic reticulum and newly synthesized EPA is then imported into the plastids for incorporation into galactolipids via an unknown route. The basis for the flux of EPA is fundamental to understanding LC-PUFA biosynthesis in diatoms. We used P. tricornutum to study acyl modifying activities, upstream of 18:1n9, on subsequent LCPUFA biosynthesis. We identified the gene coding for the plastidial acyl carrier protein D9-desaturase, a key enzyme in fatty acid modification and analyzed the impact of overexpression and knock out of this gene on
glycerolipid metabolism. This revealed a previously unknown role of this soluble desaturase in EPA synthesis and production of triacylglycerol. This study provides further insight into the distinctive nature of lipid metabolism in the marine diatom P. tricornutum and suggests additional approaches for tailoring oil composition in micro

KeywordsPhaeodactylum tricornutum; Delta 9-desaturase; Eicosapentaenoic acid; Omega-3 PUFA biosynthesis; Lipids
Year of Publication2021
JournalPlant Journal
Digital Object Identifier (DOI)https://doi.org/10.1111/tpj.15231
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeTPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry
Designing Future Wheat - WP1 - Increased efficiency and sustainability
BB/L002957/1
Publisher's version
Output statusPublished
Publication dates
Online16 Mar 2021
PublisherWiley

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