Exploring the diversity of promoter and 5’UTR sequences in ancestral, historic and modern wheat

A - Papers appearing in refereed journals

Hammond-Kosack, M., King, R., Kanyuka, K. and Hammond-Kosack, K. E. 2021. Exploring the diversity of promoter and 5’UTR sequences in ancestral, historic and modern wheat . Plant Biotechnology Journal. https://doi.org/10.1111/pbi.13672

AuthorsHammond-Kosack, M., King, R., Kanyuka, K. and Hammond-Kosack, K. E.
Abstract

A dataset of promoter and 5’UTR sequences of homoeo-alleles of 495 wheat genes that contribute to agriculturally important traits in 95 ancestral and commercial wheat cultivars is presented here. The high stringency myBaits technology used made individual capture of homoeo-allele promoters possible, which is reported here for the first time. Promoters of most genes are remarkably conserved across the 82 hexaploid cultivars used with <7 haplotypes per promoter and 21% being identical to the reference Chinese Spring. InDels and many high-confidence SNPs are located within predicted plant transcription factor binding sites, potentially changing gene expression. Most haplotypes found in the Watkins landraces and a few haplotypes found in T. monococcum, germplasms hitherto not thought to have been used in modern wheat breeding, are already found in many commercial hexaploid wheats. The full dataset which is useful for genomic and gene function studies and wheat breeding is available at https://rrescloud.rothamsted.ac.uk/index.php/s/3vc9QopcqYEbIUs/authe...

KeywordsPromoter capture; Triticum aestivum,; Triticum monococcum; Watkins landraces; Agronomic traits; Sequence variation; Haplotypes; Transposable elements (TE); Repetitive elements (RE); Transcription factor binding sites (TFBS)
Year of Publication2021
JournalPlant Biotechnology Journal
Digital Object Identifier (DOI)https://doi.org/10.1111/pbi.13672
Web address (URL)https://www.WGIN.org.uk
Open accessPublished as ‘gold’ (paid) open access
FunderDepartment of Environment, Food and Rural Affairs
Biotechnology and Biological Sciences Research Council
Funder project or codeBB/J/00426X/1
20:20 Wheat [ISPG]
BB/P016855/1)
Designing Future Wheat
BB/J/00426X/1
Accepted author manuscript
Accepted author manuscript
Output statusOther
Publication dates
Online21 Jul 2021
PublisherWiley
ISSN1467-7644

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