Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency than Parental Lines by F1 Microspore Embryogenesis

A - Papers appearing in refereed journals

Xu, H., Li, Y., Gao, R., Xu, R., Guo, G., Lu, R., Halford, N. G., Chen, Z. and Liu, C. 2021. Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency than Parental Lines by F1 Microspore Embryogenesis. Plants - Basel. 10 (8), p. 1588. https://doi.org/10.3390/plants10081588

AuthorsXu, H., Li, Y., Gao, R., Xu, R., Guo, G., Lu, R., Halford, N. G., Chen, Z. and Liu, C.
Abstract

Creating varieties with high nitrogen use efficiency (NUE) is crucial for sustainable agriculture development. In this study, a superior barley doubled haploid line (named DH45) with improved NUE was produced via F1 microspore embryogenesis with three rounds of screening in different nitrogen levels by hydroponic and field experiments. The molecular mechanisms responsible for the NUE of DH45 surpassing that of its parents were investigated by RNA-seq analysis. 1027 differentially-expressed genes (DEGs) were identified that were up- or down-regulated in DH45 under low nitrogen conditions but showed no significant differences in the parents. GO analysis indicated that genes involved in nitrogen compound metabolic processes were significantly enriched in DH45 compared with the parents. KEGG analysis showed the MAPK signaling pathway-plant to be highly enriched in DH45 relative to its parents, as well as genes involved in alanine, aspartate and glutamate metabolism, and arginine biosynthesis. In conclusion, our study revealed the potential to fix trait superiority in a line by combining crossing with F1 microspore culture technologies in future crop breeding, and also identified several candidate genes that are expressed in shoots and may enable barley to cope with low-nitrogen stress.

KeywordsBarley; Microspore; Doubled haploid line; Nitrogen use efficiency; RNA-seq
Year of Publication2021
JournalPlants - Basel
Journal citation10 (8), p. 1588
Digital Object Identifier (DOI)https://doi.org/10.3390/plants10081588
Web address (URL)https://www.mdpi.com/2223-7747/10/8/1588
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
Publisher's version
Output statusPublished
Publication dates
Online01 Aug 2021
Publication process dates
Accepted28 Jul 2021
PublisherMDPI
ISSN2223-7747

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