Identification of Candidate Carboxylesterases Associated With Odorant Degradation in Holotrichia parallela Antennae Based on Transcriptome Analysis

A - Papers appearing in refereed journals

Yi, J., Wang, S., Wang, Z., Wang, X., Gongfeng, L., Zhang, X., Pan, Y., Zhao, S., Zhang, J., Zhou, J-J., Wang, J. and Xi, J. 2021. Identification of Candidate Carboxylesterases Associated With Odorant Degradation in Holotrichia parallela Antennae Based on Transcriptome Analysis. Frontiers in Physiology. 12, p. 674023. https://doi.org/10.3389/fphys.2021.674023

AuthorsYi, J., Wang, S., Wang, Z., Wang, X., Gongfeng, L., Zhang, X., Pan, Y., Zhao, S., Zhang, J., Zhou, J-J., Wang, J. and Xi, J.
Abstract

Insects rely on their olfactory systems in antennae to recognize sex pheromones and plant volatiles in surrounding environments. Some carboxylesterases (CXEs) are odorant-degrading enzymes (ODEs), degrading odorant signals to protect the olfactory neurons against continuous excitation. However, there is no report about CXEs in Holotrichia parallela, one of the most major agricultural underground pests in China. In the present study, 20 candidate CXEs were identified based on transcriptome analysis of female and male antennae. Sequence alignments and phylogenetic analysis were performed to investigate the characterization of these candidate CXEs. The expression profiles of CXEs were compared by RT-qPCR analysis between olfactory and non-olfactory tissues of both genders. HparCXE4, 11, 16, 17, 18, 19, and 20 were antenna-biased expressed genes, suggesting their possible roles as ODEs. HparCXE6, 10, 11, 13, and 16 showed significantly higher expression profiles in male antennae, whereas HparCXE18 was expressed more in female antennae. This study highlighted candidate CXE genes linked to odorant degradation in antennae, and provided a useful resource for further work on the H. parallela olfactory mechanism and selection of target genes for integrative control of H. parallela.

KeywordsHolotrichia parallela; Antenna-biased expression profile; Antennal transcriptome; Odorant-degrading enzyme; Carboxylesterase
Year of Publication2021
JournalFrontiers in Physiology
Journal citation12, p. 674023
Digital Object Identifier (DOI)https://doi.org/10.3389/fphys.2021.674023
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Publisher's version
Output statusPublished
Publication dates
Online10 Sep 2021
Publication process dates
Accepted28 Feb 2021
ISSN1664-042X
PublisherFrontiers Media SA

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