Epidemiological studies of pan-azole resistant Aspergillus fumigatus populations sampled during tulip cultivation show clonal expansion with acquisition of multi-fungicide resistance as potential driver

A - Papers appearing in refereed journals

Fraaije, B. A., Atkins, S. L., Santos, R. F., Hanley, S. J., West, J. S. and Lucas, J. 2021. Epidemiological studies of pan-azole resistant Aspergillus fumigatus populations sampled during tulip cultivation show clonal expansion with acquisition of multi-fungicide resistance as potential driver . Microorganisms. 9 (11), p. 2379. https://doi.org/10.3390/microorganisms9112379

AuthorsFraaije, B. A., Atkins, S. L., Santos, R. F., Hanley, S. J., West, J. S. and Lucas, J.
Abstract

Pan-azole resistant isolates are found in clinical and environmental Aspergillus fumigatus (Af) populations. Azole resistance can evolve in both settings, with Af directly targeted by antifungals in patients and, in the environment, Af unintendedly exposed to fungicides used for material preservation and plant disease control. Resistance to non-azole fungi-cides, including methyl benzimidazole carbamates (MBCs), quinone outside inhibitors (QoIs) and succinate dehydrogenase inhibitors (SDHIs), have recently been reported. These fungicide groups are not used in medicine but can play an important role in further spread of pan-azole resistant genotypes. We investigated the multi-fungicide resistance status and genetic diversity of Af populations sampled from tulip field soils, tulip peel waste and flower compost heaps using fungicide sensitivity testing and a range of genotyping tools, including STRAf typing and sequencing of fungicide resistant alleles. Two major clones were present in the tulip bulb population. Comparisons with clinical isolates and literature data revealed that several common clonal lineages of TR34/L98H and TR46/Y121F/T289A strains that have expanded successfully in the environment have also acquired resistance to MBC, QoI and/or SDHI fungicides. Strains carrying multiple fungicide resistant alleles have an advantage in environments where residues of multiple fungicides belonging to different modes of action are present

KeywordsFungicide resistance ; Azole fungicides; Fungicide target proteins; CYP51A; Aspergillosis; Aspergillus fumigatus; Clonal lineages
Year of Publication2021
JournalMicroorganisms
Journal citation9 (11), p. 2379
Digital Object Identifier (DOI)https://doi.org/10.3390/microorganisms9112379
Open accessPublished as ‘gold’ (paid) open access
FunderBBSRC Industrial Strategy Challenge
Natural Environment Research Council
Funder project or codeS5402
RP10197-01
Publisher's version
Accepted author manuscript
Output statusPublished
Publication dates
Online18 Nov 2021
Publication process dates
Accepted14 Nov 2021
PublisherMDPI

Permalink - https://repository.rothamsted.ac.uk/item/98716/epidemiological-studies-of-pan-azole-resistant-aspergillus-fumigatus-populations-sampled-during-tulip-cultivation-show-clonal-expansion-with-acquisition-of-multi-fungicide-resistance-as-potential

8 total views
2 total downloads
8 views this month
0 downloads this month
Download files as zip