Chimeric Investigations into the Diamide Binding Site on the Lepidopteran Ryanodine Receptor

A - Papers appearing in refereed journals

Richardson, E., Troczka, B. J., Gutbrod, O., Ebbinghaus-Kintscher, U., Williamson, M. S., George, C.H., Nauen, R. and Davies, T. G. E. 2021. Chimeric Investigations into the Diamide Binding Site on the Lepidopteran Ryanodine Receptor. International Journal Of Molecular Sciences. 22 (23), p. 130333. https://doi.org/10.3390/ijms222313033

AuthorsRichardson, E., Troczka, B. J., Gutbrod, O., Ebbinghaus-Kintscher, U., Williamson, M. S., George, C.H., Nauen, R. and Davies, T. G. E.
Abstract

Alterations to amino acid residues G4946 and I4790, associated with resistance to diamide insecticides, suggests a location of diamide interaction within the pVSD voltage sensor-like domain of the insect ryanodine receptor (RyR). To further delineate the interaction site(s), targeted alterations were made within the same pVSD region on the diamondback moth (Plutella xylostella) RyR channel. The editing of five amino acid positions to match those found in the diamide insensitive skeletal RyR1 of humans (hRyR1) in order to generate a human–Plutella chimeric construct showed that these alterations strongly reduce diamide efficacy when introduced in combination but cause only minor reductions when introduced individually. It is concluded that the sites of diamide interaction on insect RyRs lie proximal to the voltage sensor-like domain of the RyR and that the main site of interaction is at residues K4700, Y4701, I4790 and S4919 in the S1 to S4 transmembrane domains.

KeywordsDiamide insecticides; Chlorantraniliprole; Flubendiamide; Lepidoptera; Plutella xylostella; Binding site
Year of Publication2021
JournalInternational Journal Of Molecular Sciences
Journal citation22 (23), p. 130333
Digital Object Identifier (DOI)https://doi.org/10.3390/ijms222313033
Web address (URL)https://www.mdpi.com/1422-0067/22/23/13033
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Bayer Crop Science
Funder project or codeInvestigation and characterisation of diamide insecticide resistance due to target site mutations in the ryanodine receptor of lepidopteran pests
Publisher's version
Output statusPublished
Publication dates
Online02 Dec 2021
Publication process dates
Accepted29 Nov 2021
PublisherMDPI
ISSN1422-0067

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