A - Papers appearing in refereed journals
Richardson, E., Troczka, B. J., Gutbrod, O., Ebbinghaus-Kintscher, U., Williamson, M. S., George, C.H., Nauen, R. and Davies, T. G. E. 2021. Chimeric Investigations into the Diamide Binding Site on the Lepidopteran Ryanodine Receptor. International Journal Of Molecular Sciences. 22 (23), p. 130333. https://doi.org/10.3390/ijms222313033
Authors | Richardson, E., Troczka, B. J., Gutbrod, O., Ebbinghaus-Kintscher, U., Williamson, M. S., George, C.H., Nauen, R. and Davies, T. G. E. |
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Abstract | Alterations to amino acid residues G4946 and I4790, associated with resistance to diamide insecticides, suggests a location of diamide interaction within the pVSD voltage sensor-like domain of the insect ryanodine receptor (RyR). To further delineate the interaction site(s), targeted alterations were made within the same pVSD region on the diamondback moth (Plutella xylostella) RyR channel. The editing of five amino acid positions to match those found in the diamide insensitive skeletal RyR1 of humans (hRyR1) in order to generate a human–Plutella chimeric construct showed that these alterations strongly reduce diamide efficacy when introduced in combination but cause only minor reductions when introduced individually. It is concluded that the sites of diamide interaction on insect RyRs lie proximal to the voltage sensor-like domain of the RyR and that the main site of interaction is at residues K4700, Y4701, I4790 and S4919 in the S1 to S4 transmembrane domains. |
Keywords | Diamide insecticides; Chlorantraniliprole; Flubendiamide; Lepidoptera; Plutella xylostella; Binding site |
Year of Publication | 2021 |
Journal | International Journal Of Molecular Sciences |
Journal citation | 22 (23), p. 130333 |
Digital Object Identifier (DOI) | https://doi.org/10.3390/ijms222313033 |
Web address (URL) | https://www.mdpi.com/1422-0067/22/23/13033 |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Bayer Crop Science | |
Funder project or code | Investigation and characterisation of diamide insecticide resistance due to target site mutations in the ryanodine receptor of lepidopteran pests |
Publisher's version | |
Output status | Published |
Publication dates | |
Online | 02 Dec 2021 |
Publication process dates | |
Accepted | 29 Nov 2021 |
Publisher | MDPI |
ISSN | 1422-0067 |
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