Integration of embryo–endosperm interaction into a holistic and dynamic picture of seed development using a rice mutant with notched-belly kernels

A - Papers appearing in refereed journals

Tao, Y., Xiao, F., Li, G., Ding, Y., Paul, M. J. and Liu, Z. 2021. Integration of embryo–endosperm interaction into a holistic and dynamic picture of seed development using a rice mutant with notched-belly kernels. The Crop Journal. https://doi.org/10.1016/j.cj.2021.10.007

AuthorsTao, Y., Xiao, F., Li, G., Ding, Y., Paul, M. J. and Liu, Z.
Abstract

Interaction between the embryo and endosperm affects seed development, an essential process in yield formation in crops such as rice. Signals that mediate communication between embryo and endosperm are largely unknown. We used the notched-belly (NB) mutant with impaired communication between embryo and endosperm to investigate the effect of the embryo on developmental staging of the endosperm and signaling pathways in the embryo that regulate endosperm development. Hierachical clustering of mRNA datasets from embryo and endosperm samples collected during development in NB and a wild type showed a delaying effect of the embryo on the developmental transition of the endosperm by extension of the middle stage. K-means clustering further identified coexpression modules of gene sets specific to embryo and endosperm development. Combined gene expression and biochemical analysis showed that T6P–SnRK1, gibberellin and auxin signaling by the embryo regulate endosperm developmental transition. We propose a new seed developmental staging system for rice and identify the most detailed signature of rice grain formation to date. These will direct genetic strategies for rice yield improvement.

KeywordsEmbryo–endosperm interaction ; Developmental transition; Transcriptome; Sugar signalling; Rice physiology; Seed development
Year of Publication2021
JournalThe Crop Journal
Digital Object Identifier (DOI)https://doi.org/10.1016/j.cj.2021.10.007
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
Accepted author manuscript
Output statusPublished
Publication dates
Online06 Dec 2021
Publication process dates
Accepted28 Oct 2021
PublisherElsevier
ISSN2214-5141

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