Reduction effect of individual N, P, K fertilization on antibiotic resistance genes in reclaimed water irrigated soil

A - Papers appearing in refereed journals

Cui, E., Fan, X., Hu, C., Neal, A. L., Cui, B., Liu, C. and Gao, F. 2022. Reduction effect of individual N, P, K fertilization on antibiotic resistance genes in reclaimed water irrigated soil . Ecotoxicology and Environmental Safety. 231, p. 113185. https://doi.org/10.1016/j.ecoenv.2022.113185

AuthorsCui, E., Fan, X., Hu, C., Neal, A. L., Cui, B., Liu, C. and Gao, F.
Abstract

The transfer of antibiotic resistance genes (ARGs) in soil under reclaimed water irrigation poses a potential environmental risk. Regulation of NPK fertilizer could influence the behavior of bacterial communities, mobile genetic elements (MGEs), and soil properties, which determine the fate of ARGs. To identify the key element in NPK fertilizer and realize efficient regulation, we explored the effect of individual N, P, K fertilization on ARGs variation in tomato rhizosphere and bulk soils. Compared with an unfertilized treatment, N fertilization resulted in greater decreases in the abundance of ARGs (decreases of 24.06%‒73.09%) than did either P fertilization (increases of up to 35.84%, decreases of up to 58.80%) or K fertilization (decreases of 13.47%‒72.47%). The influence of different forms of N (CO(NH2)2, NaNO3, and NH4HCO3), P (Ca(H2PO4)2 and CaMgO4P+), and K (KCl and K2(SO4)) fertilizers was also investigated in this study, and showed the influence of NaNO3, CaMgO4P+, and K2(SO4) on reducing ARGs abundance was greater in different types of N, P, K fertilizers. Bacterial communities showed the strongest response to N fertilization. The reduced bacterial diversity and abundance of ARG-host and non-host organisms explained the decline of total ARG abundance in soil. In soils fertilized with either P or K, the effect of soil properties, especially total nitrogen and pH, on ARGs variation was greater than that of bacterial community and MGEs. These results suggest that N regulation of in NPK fertilizer may be an effective way to reduce the risks of ARGs in soil associated with reclaimed water irrigation.

KeywordsChemical fertilizer; Reclaimed water irrigation; Antibiotic resistance genes; Bacterial community
Year of Publication2022
JournalEcotoxicology and Environmental Safety
Journal citation231, p. 113185
Digital Object Identifier (DOI)https://doi.org/10.1016/j.ecoenv.2022.113185
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Publisher's version
Accepted author manuscript
Supplemental file
Output statusPublished
Publication dates
Online12 Jan 2022
Publication process dates
Accepted09 Jan 2022
PublisherAcademic Press Inc Elsevier Science
ISSN0147-6513

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