Super-resolution Chromatin Visualization Using a Combined Method of Fluorescence In Situ Hybridization and Structured Illumination Microscopy in Solanum lycopersicum

B - Book chapters etc edited externally

Lambing, C. 2022. Super-resolution Chromatin Visualization Using a Combined Method of Fluorescence In Situ Hybridization and Structured Illumination Microscopy in Solanum lycopersicum. in: Lambing, C. (ed.) Plant Gametogenesis Springer Nature. pp. 85-92

AuthorsLambing, C.
EditorsLambing, C.
Abstract

Chromatin organization influences gene and transposon expression, and regulates various cellular processes. Higher order chromatin structure has been widely studied using genomic approaches and microscopy image analyses. Chromosome conformation capture and sequencing the junction of DNA fragments enables the study of both chromatin interaction and chromosome folding. However, certain cell types are embedded in other cell types which complicate the process of studying them using high-throughput genomic approaches. To overcome this limitation, high-resolution microscopy techniques are now available to investigate chromatin organization in single cells. In this chapter, we provide a detailed protocol to prepare chromosome spreading from tomato nuclei, to label genomic loci by fluorescence in situ hybridization, and to visualize these locations at high resolution with Structured Illumination microscopy.

KeywordsChromatin; Fluorescence in situ hybridization; Meiosis; Recombination; SIM microscopy; Solanum lycopersicum; Super-resolution
Page range85-92
Year of Publication2022
Book titlePlant Gametogenesis
PublisherSpringer Nature
Edition1
SeriesMethods in Molecular Biology book series (MIMB, volume 2484)
ISBN978-1-0716-2253-7
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-0716-2253-7_7
PubMed ID35461446
Web address (URL)https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_7
FunderBiotechnology and Biological Sciences Research Council
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online24 Apr 2022

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