Integration of embryo–endosperm interaction into a holistic and dynamic picture of seed development using a rice mutant with notched-belly kernels

A - Papers appearing in refereed journals

Tao, Y., An, L., Xiao, F., Lia, G, Ding, Y, Paul, M. J. and Liu, Z. 2022. Integration of embryo–endosperm interaction into a holistic and dynamic picture of seed development using a rice mutant with notched-belly kernels. The Crop Journal. 10, pp. 729-742. https://doi.org/10.1016/j.cj.2021.10.007

AuthorsTao, Y., An, L., Xiao, F., Lia, G, Ding, Y, Paul, M. J. and Liu, Z.
Abstract

Interaction between the embryo and endosperm affects seed development, an essential process in yield formation in crops such as rice. Signals that mediate communication between embryo and endosperm are largely unknown. We used the notched-belly (NB) mutant with impaired communication between embryo and endosperm to investigate the effect of the embryo on developmental staging of the endosperm and signaling pathways in the embryo that regulate endosperm development. Hierachical clustering of mRNA datasets from embryo and endosperm samples collected during development in NB and a wild type showed a delaying effect of the embryo on the developmental transition of the endosperm by extension of the middle stage. K-means clustering further identified coexpression modules of gene sets specific to embryo and endosperm development. Combined gene expression and biochemical analysis showed that T6P–SnRK1, gibberellin and auxin signaling by the embryo regulate endosperm developmental transition. We propose a new seed developmental staging system for rice and identify the most detailed signature of rice grain formation to date. These will direct genetic strategies for rice yield improvement.

KeywordsSeed development; Embryo-endosperm interaction; Developmental transition; Transcriptome; Sugar signaling; Rice physiology
Year of Publication2022
JournalThe Crop Journal
Journal citation10, pp. 729-742
Digital Object Identifier (DOI)https://doi.org/10.1016/j.cj.2021.10.007
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat - WP1 - Increased efficiency and sustainability
Publisher's version
Output statusPublished
Publication dates
Online01 Jun 2022
PublisherElsevier
ISSN2214-5141

Permalink - https://repository.rothamsted.ac.uk/item/989q1/integration-of-embryo-endosperm-interaction-into-a-holistic-and-dynamic-picture-of-seed-development-using-a-rice-mutant-with-notched-belly-kernels

37 total views
4 total downloads
2 views this month
0 downloads this month
Download files as zip