Inhibition of Rice Germination by Ustiloxin A Involves Alteration in Carbon Metabolism and Amino Acid Utilization

A - Papers appearing in refereed journals

Fu, X., Jin, Y., Paul, M. J., Yuan, M., Liang, X., Cui, R., Huang, Y-J., Peng, W. and Liang, X. 2023. Inhibition of Rice Germination by Ustiloxin A Involves Alteration in Carbon Metabolism and Amino Acid Utilization. Frontiers in Plant Science. 14, p. 1168985. https://doi.org/10.3389/fpls.2023.1168985

AuthorsFu, X., Jin, Y., Paul, M. J., Yuan, M., Liang, X., Cui, R., Huang, Y-J., Peng, W. and Liang, X.
Abstract

Ustiloxins are the main mycotoxin in rice false smut, a devastating disease caused by Ustilaginoidea virens. A typical phytotoxicity of ustiloxins is strong inhibition of seed germination, but the physiological mechanism is not clear. Here, we show that the inhibition of rice germination by ustiloxin A (UA) is dose-dependent. The sugar availability in UA-treated embryo was lower while the starch residue in endosperm was higher. The transcripts and metabolites responsive to typical UA treatment were investigated. The expression of several SWEET genes responsible for sugar transport in embryo was down-regulated by UA. Glycolysis and pentose phosphate processes in embryo were transcriptionally repressed. Most of the amino acids detected in endosperm and embryo were variously decreased. Ribosomal RNAs for growth was inhibited while secondary metabolites salicylic acid was also decreased under UA. Hence, we propose that the inhibition of seed germination by UA involves the block of sugar transport from endosperm to embryo, leading to altered carbon metabolism and amino acids utilization in rice plants. Our analysis provides a framework for understanding of the molecular mechanisms of ustiloxins on rice growth and in pathogen infection.

KeywordsSeed germination; Carbon Metabolism; Ustiloxins; Rice false smut; Sugar transport
Year of Publication2023
JournalFrontiers in Plant Science
Journal citation14, p. 1168985
Digital Object Identifier (DOI)https://doi.org/10.3389/fpls.2023.1168985
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 1 (WP1) - Increased efficiency and sustainability
Publisher's version
Output statusPublished
Publication dates
Online08 May 2023
Publication process dates
Accepted11 Apr 2023
PublisherFrontiers Media SA
ISSN1664-462X

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