Pea aphid odorant-binding protein ApisOBP6 discriminates between aphid sex pheromone components, aphid alarm pheromone and a host plant volatile

A - Papers appearing in refereed journals

Sims, C., Birkett, M. A., Oldham, N. J., Stockman, R. A. and Withall, D. M 2023. Pea aphid odorant-binding protein ApisOBP6 discriminates between aphid sex pheromone components, aphid alarm pheromone and a host plant volatile. Insect Biochemistry and Molecular Biology. 162, p. 104026. https://doi.org/10.1016/j.ibmb.2023.104026

AuthorsSims, C., Birkett, M. A., Oldham, N. J., Stockman, R. A. and Withall, D. M
Abstract

Olfactory perception of pheromones in insects involves odorant-binding proteins (OBPs), relatively small proteins (ca.110-240 amino acid residues) that can bind reversibly to behaviourally active olfactory ligands. In this study, we investigated the binding in silico and in vitro of the aphid sex pheromone components (1R,4aS,7S,7aR)-nepetalactol and (4aS,7S,7aR)-nepetalactone and the aphid alarm pheromone (E)-β-farnesene by OBPs from the pea aphid, Acyrthosiphon pisum. Screening of protein models of ApisOBPs1-11 with the aphid sex pheromone components suggested that ApisOPB6 was a candidate. Fluorescence assays using ApisOBP6 suggested that ApisOBP6 was able to bind both sex pheromone components and discriminate from the aphid alarm pheromone and the generic plant compound (R/S)-linalool. Saturation transfer difference STD-NMR experiments with ApisOBP6 yielded results consistent to those from the fluorescence experiments, with a clear interaction between ApisOBP6 and (4aS,7S,7aR)-nepetalactone. These results describe a novel interaction and potential function for ApisOBP6, point to pre-receptor odorant discrimination by OBPs, and provide a platform for investigating the function of other aphid olfactory proteins involved in aphid chemical ecology.

KeywordsInsect olfaction; Protein NMR; Aphids; Pheromones; Molecular docking; Odorant-binding protein
Year of Publication2023
JournalInsect Biochemistry and Molecular Biology
Journal citation162, p. 104026
Digital Object Identifier (DOI)https://doi.org/10.1016/j.ibmb.2023.104026
Web address (URL)https://www.sciencedirect.com/science/article/pii/S0965174823001200?via%3Dihub
Open accessPublished as ‘gold’ (paid) open access
FunderBBSRC Industrial Strategy Challenge
Biotechnology and Biological Sciences Research Council
Funder project or codeGrowing Health [ISP]
BBSRC Strategic Programme in Smart Crop Protection
BBSRC Notts DTP
Growing Health (WP1) - bio-inspired solutions for healthier agroecosystems: Understanding biointeractions
Publisher's version
Accepted author manuscript
Supplemental file
Output statusPublished
Publication dates
Online11 Oct 2023
Publication process dates
Accepted08 Oct 2023
PublisherElsevier
ISSN0965-1748

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