Characterisation of fatty acyl reductases of sunflower (Helianthus annuus L.) seed

A - Papers appearing in refereed journals

DeAndres-Gil, C., Moreno-Perez, A. J., Villoslada-Valbuena, M., Halsey, K., Martinez-Force, E., Garces, R., Kurup, S., Beaudoin, F., Salas, J. J. and Venegas-Caleron, M. 2024. Characterisation of fatty acyl reductases of sunflower (Helianthus annuus L.) seed. Plant Science. 341, p. 111992. https://doi.org/10.1016/j.plantsci.2024.111992

AuthorsDeAndres-Gil, C., Moreno-Perez, A. J., Villoslada-Valbuena, M., Halsey, K., Martinez-Force, E., Garces, R., Kurup, S., Beaudoin, F., Salas, J. J. and Venegas-Caleron, M.
Abstract

Long and very long chain fatty alcohols are produced from their corresponding acyl-CoAs through the activity of fatty acyl reductases (FARs). Fatty alcohols are important components of the cuticle that protects aerial plant organs, and they are metabolic intermediates in the synthesis of the wax esters in the hull of sunflower (Helianthus annuus) seeds. Genes encoding 4 different FARs (named HaFAR2, HaFAR3, HaFAR4 and HaFAR5) were identified using BLAST, and studies showed that four of the genes were expressed in seed hulls. In this study, the structure and location of sunflower FAR proteins were determined. They were also expressed exogenously in Saccharomyces cerevisiae to evaluate their substrate specificity based on the fatty alcohols synthesized by the transformed yeasts. Three of the four enzymes tested showed activity in yeast. HaFAR3 produced C18, C20 and C22 saturated alcohols, whereas HaFAR4 and HaFAR5 produced C24 and C26 saturated alcohols. The involvement of these genes in the synthesis of sunflower seed wax esters was addressed by considering the results obtained.

KeywordsSunflower oil; Wax ester; FAR; Fatty acyl-CoA reductase
Year of Publication2024
JournalPlant Science
Journal citation341, p. 111992
Digital Object Identifier (DOI)https://doi.org/10.1016/j.plantsci.2024.111992
Open accessPublished as ‘gold’ (paid) open access
Publisher's version
Output statusPublished
Publication dates
Online30 Jan 2024
Publication process dates
Accepted14 Jan 2024
PublisherElsevier
ISSN0168-9452

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