D - Report
Thomas, G., Mohan-Kumar, A., Borg, A., Caulfield, J. C., Birkett, M. A. and Vuts, J. 2024. Solvent Extraction of Polydimethylsiloxane (PDMS) Tubing to Capture Volatile Organic Compounds from the Headspace and the Rhizosphere. protocols.io https://doi.org/10.17504/protocols.io.eq2lyjmqrlx9/v1
Authors | Thomas, G., Mohan-Kumar, A., Borg, A., Caulfield, J. C., Birkett, M. A. and Vuts, J. |
---|---|
Abstract | This protocol describes a step-by-step guide for sampling Volatile Organic Compounds (VOCs) from the headspace/rhizosphere for synthetic blends and biological samples using polydimethylsiloxane (PDMS) silicon tubing, based on methods reported in Thomas et al. (2024) and Vuts et al. (2020). The advantages of using solvent elution when sampling headspace with PDMS tubing are that a liquid sample is generated, which can be used for multiple chemical (e.g. Gas Chromatography/coupled GC-Mass Spectrometry/Nuclear Mass Resonance Spectroscopy), behavioural (e.g. olfactometry) and electrophysiological (e.g. GC-electroantennography) tests, enabling a potential link to be established between VOC content and biological activity. |
Keywords | Chemical Ecology; Semiochemicals; Natural Products; Volatile Organic Compounds; Headspace; Rhizosphere; PDMS; Polydimethylsiloxane |
Year of Publication | 2024 |
Place of publication | protocols.io |
Digital Object Identifier (DOI) | https://doi.org/10.17504/protocols.io.eq2lyjmqrlx9/v1 |
Web address (URL) | https://www.protocols.io/view/solvent-extraction-of-polydimethylsiloxane-pdms-tu-dai32cgn.html |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Growing Health [ISP] |
Growing Health (WP1) - bio-inspired solutions for healthier agroecosystems: Understanding biointeractions | |
Semiochemical-based alternative concepts for the management of wireworms | |
The Electrostatic Basis of Insect Olfaction | |
Open access | Published as green open access |
Accepted author manuscript | |
Output status | Published |
Publication dates | |
Online | 22 Mar 2024 |
Publication process dates | |
Accepted | 22 Mar 2024 |
Permalink - https://repository.rothamsted.ac.uk/item/98zvy/solvent-extraction-of-polydimethylsiloxane-pdms-tubing-to-capture-volatile-organic-compounds-from-the-headspace-and-the-rhizosphere