Solvent Extraction of Polydimethylsiloxane (PDMS) Tubing to Capture Volatile Organic Compounds from the Headspace and the Rhizosphere

D - Report

Thomas, G., Mohan-Kumar, A., Borg, A., Caulfield, J. C., Birkett, M. A. and Vuts, J. 2024. Solvent Extraction of Polydimethylsiloxane (PDMS) Tubing to Capture Volatile Organic Compounds from the Headspace and the Rhizosphere. protocols.io https://doi.org/10.17504/protocols.io.eq2lyjmqrlx9/v1

AuthorsThomas, G., Mohan-Kumar, A., Borg, A., Caulfield, J. C., Birkett, M. A. and Vuts, J.
Abstract

This protocol describes a step-by-step guide for sampling Volatile Organic Compounds (VOCs) from the headspace/rhizosphere for synthetic blends and biological samples using polydimethylsiloxane (PDMS) silicon tubing, based on methods reported in Thomas et al. (2024) and Vuts et al. (2020). The advantages of using solvent elution when sampling headspace with PDMS tubing are that a liquid sample is generated, which can be used for multiple chemical (e.g. Gas Chromatography/coupled GC-Mass Spectrometry/Nuclear Mass Resonance Spectroscopy), behavioural (e.g. olfactometry) and electrophysiological (e.g. GC-electroantennography) tests, enabling a potential link to be established between VOC content and biological activity.

KeywordsChemical Ecology; Semiochemicals; Natural Products; Volatile Organic Compounds; Headspace; Rhizosphere; PDMS; Polydimethylsiloxane
Year of Publication2024
Place of publicationprotocols.io
Digital Object Identifier (DOI)https://doi.org/10.17504/protocols.io.eq2lyjmqrlx9/v1
Web address (URL)https://www.protocols.io/view/solvent-extraction-of-polydimethylsiloxane-pdms-tu-dai32cgn.html
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeGrowing Health [ISP]
Growing Health (WP1) - bio-inspired solutions for healthier agroecosystems: Understanding biointeractions
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Open accessPublished as green open access
Accepted author manuscript
Output statusPublished
Publication dates
Online22 Mar 2024
Publication process dates
Accepted22 Mar 2024

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