BIG participates in the Arg/N-degron pathways and the hypoxia response in Arabidopsis thaliana

A - Papers appearing in refereed journals

Zhang, H., Rundle, C., Winter, N., Miricescu, A., Mooney B. C., Bachmair, A., Graciet, E. and Theodoulou, F. L. 2024. BIG participates in the Arg/N-degron pathways and the hypoxia response in Arabidopsis thaliana. The Plant Cell. p. koae117. https://doi.org/10.1093/plcell/koae117

AuthorsZhang, H., Rundle, C., Winter, N., Miricescu, A., Mooney B. C., Bachmair, A., Graciet, E. and Theodoulou, F. L.
Abstract

BIG/DARK OVEREXPRESSION OF CAB1/TRANSPORT INHIBITOR RESPONSE3 is a 0.5-MDa protein associated with multiple functions in Arabidopsis (Arabidopsis thaliana) signalling and development. However, the biochemical functions of BIG are unknown. We investigated a role for BIG in the Arg/N-degron pathways, in which substrate protein fate is influenced by the N-terminal (Nt) residue. We crossed a big loss-of-function allele to two N-degron pathway E3 ligase mutants, proteolysis6 (prt6) and prt1, and examined the stability of protein substrates. Stability of model substrates was enhanced in prt6-1 big-2 and prt1-1 big-2 relative to the respective single mutants and the abundance of the PRT6 physiological substrates, HYPOXIA-RESPONSIVE ERF2 (HRE2) and VERNALIZATION2 (VRN2) was similarly increased in prt6 big double mutants. Hypoxia marker expression was enhanced in prt6 big double mutants; this constitutive response required arginyltransferase activity and RAP-type ERFVII transcription factors. Transcriptomic analysis of roots not only demonstrated increased expression of multiple hypoxia-responsive genes in the double mutant relative to prt6, but also revealed other roles for PRT6 and BIG, including regulation of suberin deposition through both ERFVII-dependent and independent mechanisms, respectively. Our results show that BIG acts together with PRT6 to regulate the hypoxia response and broader processes in Arabidopsis.

KeywordsProteolosis6 (PRT6) ; Suberin; Ubiquitin amino-end recognizing protein box (UBR box) ; Proteostasis; Vernalisation (VRN2)
Year of Publication2024
JournalThe Plant Cell
Journal citationp. koae117
Digital Object Identifier (DOI)https://doi.org/10.1093/plcell/koae117
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeTailoring Plant Metabolism ISP
Green Engineering
Tailoring Plant Metabolism (TPM) - Work package 1 (WP1) - High value lipids for health and industry
Publisher's version
Output statusPublished
Publication dates
Online12 Apr 2024
Publication process dates
Accepted21 Mar 2024
PublisherAmerican Society of Plant Biologists (ASPB)
ISSN1532-298X

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