Wheat gluten proteins: their characterisation and role in determining the functional properties and end use quality of wheat

Project (past)

Project dates01 Apr 1999 to end of 31 Mar 2008
Project number4226
DepartmentCrop Performance and Improvement (CPI) Division
Plant Sciences (PSC)
Research fish codeBBS/E/C/00004226
Grant IDBBS/E/C/00004226

Outputs

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Expression of epitope-tagged LMW glutenin subunits in the starchy endosperm of transgenic wheat and their incorporation into glutenin polymers

A - Papers appearing in refereed journals
Tosi, P., D'ovidio, R., Napier, J. A., Bekes, F. and Shewry, P. R. 2004. Expression of epitope-tagged LMW glutenin subunits in the starchy endosperm of transgenic wheat and their incorporation into glutenin polymers. Theoretical and Applied Genetics. 108, pp. 468-476.

Protein extraction from plant tissues

B - Book chapters etc edited externally
Fido, R. J., Mills, E. N. C., Rigby, N. M. and Shewry, P. R. 2004. Protein extraction from plant tissues. in: Cutler, P. (ed.) Protein purification protocols, 2nd ed. Humana Press, Totowa. pp. 21-27

Structural studies of the allelic wheat glutenin subunits 1Bx7 and 1Bx20 by matrix-assisted laser desorption/ionization mass spectrometry and high-performance liquid chromatography/electrospray ionization mass spectrometry

A - Papers appearing in refereed journals
Cunsolo, V., Foti, S., Saletti, R., Gilbert, S., Tatham, A. S. and Shewry, P. R. 2004. Structural studies of the allelic wheat glutenin subunits 1Bx7 and 1Bx20 by matrix-assisted laser desorption/ionization mass spectrometry and high-performance liquid chromatography/electrospray ionization mass spectrometry. Journal of Mass Spectrometry. 39, pp. 66-78.

The use of biotechnology to study wheat endosperm development and improve grain quality

C1 - Edited contributions to conferences/learned societies
Shewry, P. R., Jones, H. D., Holdsworth, M. J., Lenton, J. R. and Edwards, K. J. 2004. The use of biotechnology to study wheat endosperm development and improve grain quality. D'ovidio, R., Lafiandra, D. and Masci, S. (ed.) Gluten Proteins. Proceedings of the 8th Gluten Workshop, Viterbo, 8-10 Septmeber 2003 . Royal Society of Chemistry (RSC), Cambridge. pp. 3-5

Specificity of action of the wheat bug (Nysius huttoni ) proteinase

C1 - Edited contributions to conferences/learned societies
Every, D., Sutton, K. H., Shewry, P. R., Tatham, A. S. and Coolbear, T. 2004. Specificity of action of the wheat bug (Nysius huttoni ) proteinase. D'ovidio, R., Lafiandra, D. and Masci, S. (ed.) Gluten Proteins. Proceedings of the 8th Gluten Workshop, Viterbo, 8-10 September 2003 . Royal Society of Chemistry (RSC), Cambridge. pp. 413-416

The high molecular weight subunits of wheat glutenin and their role in determining wheat processing qualities

A - Papers appearing in refereed journals
Shewry, P. R., Halford, N. G., Tatham, A. S., Popineau, Y., Lafiandra, D. and Belton, P. S. 2003. The high molecular weight subunits of wheat glutenin and their role in determining wheat processing qualities. Advances in Food and Nutrition Research. 45, pp. 219-302.

Manipulation of the composition and functional properties of wheat gluten by chromosome and genetic engineering

C1 - Edited contributions to conferences/learned societies
Shewry, P. R. and Lafiandra, D. 2003. Manipulation of the composition and functional properties of wheat gluten by chromosome and genetic engineering. Stanca, A. M., Mare, C. and Faccoili, P. (ed.) Proceedings Cereal Section Meeting of the European Association for Research on Plant Breeding: From Biodiversity to Genomics: Breeding Strategies for Small Grain Cereals in the Third Millennium, Salsomaggiore, 21-25 November 2002 . Experimental Institute for Cereal Research, Fiorenzuola d'Arda. pp. 313-317

Quantification of nucleic acids using real-time PCR technology: examples of application to small grain cereals

C1 - Edited contributions to conferences/learned societies
Faccioli, P., Shewry, P. R. and Terzi, V. 2003. Quantification of nucleic acids using real-time PCR technology: examples of application to small grain cereals. Stanca, A. M., Mare, C. and Faccoili, P. (ed.) Proceedings Cereal Section Meeting of the European Association for Research on Plant Breeding: From Biodiversity to Genomics: Breeding Strategies for Small Grain Cereals in the Third Millennium, Salsomaggiore, 21-25 November 2002 . Experimental Institute for Cereal Research, Fiorenzuola d'Arda. pp. 447-449

Long Ashton Research Station: one hundred years of science in support of agriculture

E - Books edited/written by staff member, alone/with others
Anderson, H. M., Lenton, J. R. and Shewry, P. R. (ed.) 2003. Long Ashton Research Station: one hundred years of science in support of agriculture. Institute for Arable Crops Research (IACR) , Long Ashton Research Station (LARS), Bristol.

From Research Station to Institute, 1975-2003

B - Book chapters etc edited externally
Brent, K. J. 2003. From Research Station to Institute, 1975-2003. in: Shewry, P. R., Lenton, J. R. and Anderson, H. M. (ed.) Long Ashton Research Station: one hundred years of science in support of agriculture Institute for Arable Crops Research (IACR) , Long Ashton Research Station (LARS), Bristol. pp. 65-76

The GM revolution

B - Book chapters etc edited externally
Holdsworth, M. J. and Halford, N. G. 2003. The GM revolution. in: Shewry, P. R., Lenton, J. R. and Anderson, H. M. (ed.) Long Ashton Research Station: one hundred years of science in support of agriculture Institute for Arable Crops Research (IACR) , Long Ashton Research Station (LARS), Bristol. pp. 312-321

Wheat gluten protein analysis

E - Books edited/written by staff member, alone/with others
Shewry, P. R. Lookhart, G. L. (ed.) 2003. Wheat gluten protein analysis. American Association of Cereal Chemists (AACC), St Paul, MN.

Wheat gluten proteins

B - Book chapters etc edited externally
Shewry, P. R. 2003. Wheat gluten proteins. in: Shewry, P. R. and Lookhart, G. L. (ed.) Wheat gluten protein analysis American Association of Cereal Chemists (AACC), St Paul, MN. pp. 1-17

Electrophoresis of wheat gluten proteins

B - Book chapters etc edited externally
Khan, K., Nygard, G., Pogna, N. E., Redaelli, R., Ng, P. K. W. and Fido, R. J. 2003. Electrophoresis of wheat gluten proteins. in: Shewry, P. R. and Lookhart, G. L. (ed.) Wheat gluten protein analysis American Association of Cereal Chemists (AACC), St Paul, MN. pp. 31-59

Elastomeric proteins: structures, biomechanical properties and biological roles

E - Books edited/written by staff member, alone/with others
Shewry, P. R. and Tatham, A. S. Bailey, A. J. (ed.) 2003. Elastomeric proteins: structures, biomechanical properties and biological roles. Cambridge University Press (CUP) Cambridge.

Comparative structures and properties of elastic proteins

B - Book chapters etc edited externally
Tatham, A. S. and Shewry, P. R. 2003. Comparative structures and properties of elastic proteins. in: Shewry, P. R., Bailey, A. J. and Tatham, A. S. (ed.) Elastomeric proteins: structures, biomechanical properties and biological roles Cambridge University Press (CUP) Cambridge. pp. 338-351

Gluten, the elastomeric protein of wheat seeds

B - Book chapters etc edited externally
Halford, N. G. and Belton, P. S. 2003. Gluten, the elastomeric protein of wheat seeds. in: Shewry, P. R., Bailey, A. J. and Tatham, A. S. (ed.) Elastomeric proteins: structures, biomechanical properties and biological roles Cambridge University Press (CUP) Cambridge. pp. 279-301

TaqMan PCR for detection of genetically modified durum wheat

A - Papers appearing in refereed journals
Terzi, V., Ferrari, B., Finocchiaro, F., Di Fonzo, N., Stanca, A. M., Lamacchia, C., Napier, J. A., Shewry, P. R. and Faccioli, P. 2003. TaqMan PCR for detection of genetically modified durum wheat. Journal of Cereal Science. 37, pp. 157-163.

Genetics of wheat gluten proteins

A - Papers appearing in refereed journals
Shewry, P. R., Halford, N. G. and Lafiandra, D. 2003. Genetics of wheat gluten proteins. Advances in Genetics. 49, pp. 111-184.

Transgene inheritance, segregation and expression in bread wheat

A - Papers appearing in refereed journals
Rooke, L., Steele, S. H., Barcelo, P., Shewry, P. R. and Lazzeri, P. A. 2003. Transgene inheritance, segregation and expression in bread wheat. Euphytica. 129, pp. 301-309.

Transgenic approaches to study quality traits in cereals

C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Sparks, C. A. and Shewry, P. R. 2003. Transgenic approaches to study quality traits in cereals. Proceedings BCPC International Congress - Crop Science and Technology, Glasgow, 10-12 November 2003 . pp. 133-140

Effects of restricted water availability and increased temperature on the grain filling, drying and quality of winter wheat

A - Papers appearing in refereed journals
Gooding, M. J., Ellis, R. H., Shewry, P. R. and Schofield, J. D. 2003. Effects of restricted water availability and increased temperature on the grain filling, drying and quality of winter wheat. Journal of Cereal Science. 37, pp. 295-309.

Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length

A - Papers appearing in refereed journals
Feeney, K. A., Wellner, N., Gilbert, S. M., Halford, N. G., Tatham, A. S., Shewry, P. R. and Belton, P. S. 2003. Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length. Biopolymers (Biospectroscopy). 72, pp. 123-131.

Milling and baking properties of field grown wheat expressing HMW subunit transgenes

A - Papers appearing in refereed journals
Darlington, H., Fido, R. F., Tatham, A. S., Jones, H. D., Salmon, S. E. and Shewry, P. R. 2003. Milling and baking properties of field grown wheat expressing HMW subunit transgenes. Journal of Cereal Science. 38, pp. 301-306.

Structural studies of glutenin subunits 1Dy10 and 1Dy12 by matrix-assisted laser desorption/ionisation mass spectrometry and high-performance liquid chromatography/electrospray ionisation mass spectrometry

A - Papers appearing in refereed journals
Cunsolo, V., Foti, S., Saletti, R., Gilbert, S. M., Tatham, A. S. and Shewry, P. R. 2003. Structural studies of glutenin subunits 1Dy10 and 1Dy12 by matrix-assisted laser desorption/ionisation mass spectrometry and high-performance liquid chromatography/electrospray ionisation mass spectrometry. Rapid Communications in Mass Spectrometry. 17, pp. 442-454.

Creating a balance - the incorporation of a HMW glutenin subunit into transgenic wheat lines

A - Papers appearing in refereed journals
Butow, B. J., Tatham, A. S., Savage, A. W. J., Gilbert, S. M., Shewry, P. R., Solomon, R. G. and Bekes, F. 2003. Creating a balance - the incorporation of a HMW glutenin subunit into transgenic wheat lines. Journal of Cereal Science. 38, pp. 181-187.

Functional properties of flours from field grown transgenic wheat lines expressing the HMW glutenin subunit 1Ax1 and 1Dx5 genes

A - Papers appearing in refereed journals
Barro, F., Barcelo, P., Lazzeri, P. A., Shewry, P. R., Ballesteros, J. and Martin, A. 2003. Functional properties of flours from field grown transgenic wheat lines expressing the HMW glutenin subunit 1Ax1 and 1Dx5 genes. Molecular Breeding. 12, pp. 223-229.

Functional properties and agronomic performance of transgenic tritordeum expressing high molecular weight glutenin subunit genes 1Ax1 and 1Dx5

A - Papers appearing in refereed journals
Barro, F., Barcelo, P., Lazzeri, P. A., Shewry, P. R., Martin, A. and Ballesteros, J. 2003. Functional properties and agronomic performance of transgenic tritordeum expressing high molecular weight glutenin subunit genes 1Ax1 and 1Dx5. Journal of Cereal Science. 37, pp. 65-70.

Improving wheat quality: the role of biotechnology

B - Book chapters etc edited externally
Shewry, P. R. 2003. Improving wheat quality: the role of biotechnology. in: Cauvain, S. P. (ed.) Bread making: improving quality Woodhead Publishing, Cambridge. pp. 168-186

Functional properties of wheat gliadins. II. Effects on dynamic rheological properties of wheat gluten

A - Papers appearing in refereed journals
Khatkar, B. S., Fido, R. J., Tatham, A. S. and Schofield, J. D. 2002. Functional properties of wheat gliadins. II. Effects on dynamic rheological properties of wheat gluten. Journal of Cereal Science. 35 (3), pp. 307-313. https://doi.org/10.1006/jcrs.2001.0430

Functional properties of wheat gliadins. I. Effects on mixing characteristics and bread making quality

A - Papers appearing in refereed journals
Khatkar, B. S., Fido, R. J., Tatham, A. S. and Schofield, J. D. 2002. Functional properties of wheat gliadins. I. Effects on mixing characteristics and bread making quality. Journal of Cereal Science. 35 (3), pp. 299-306. https://doi.org/10.1006/jcrs.2001.0429