TPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry

Repository project

Project dates01 Apr 2017 to end of 31 Mar 2022
Researchers
Principal InvestigatorFreddie Theodoulou
Co-InvestigatorJohnathan Napier
FunderBiotechnology and Biological Sciences Research Council
Project numberI0420
I0400
DepartmentPlant Sciences
Research fish codeBBS/E/C/000I0420
Grant IDBBS/E/C/000I0420

Outputs

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A causal inference and Bayesian optimisation framework for modelling multi-trait relationships—Proof-of-concept using Brassica napus seed yield under controlled conditions

A - Papers appearing in refereed journals
Calderwood, A., Siles-Suarez, L., Eastmond, P. J., Kurup, S. and Morris, R. 2023. A causal inference and Bayesian optimisation framework for modelling multi-trait relationships—Proof-of-concept using Brassica napus seed yield under controlled conditions. PLOS ONE. 18 (9), p. 0290429. https://doi.org/10.1371/journal.pone.0290429

A Field Day for Gene-Edited Brassicas and Crop Improvement

A - Papers appearing in refereed journals
Napier, J. A. 2021. A Field Day for Gene-Edited Brassicas and Crop Improvement. The CRISPR Journal. 4 (3), pp. 307-312. https://doi.org/10.1089/crispr.2021.29130.kad

A Palmitic Acid Elongase Affects Eicosapentaenoic Acid and Plastidial Monogalactosyldiacylglycerol Levels in Nannochloropsis

A - Papers appearing in refereed journals
Dolch, L-J., Rak, C., Perin, G., Tourcier, G., Broughton, R., Leterrier, M., Morosinotto, T., Tellier, F., Faure, J-D., Falconet, D., Jouhet, J., Sayanova, O. V., Beaudoin, F. and Maréchal, E. 2017. A Palmitic Acid Elongase Affects Eicosapentaenoic Acid and Plastidial Monogalactosyldiacylglycerol Levels in Nannochloropsis. Plant Physiology. 173 (1), pp. 742-759. https://doi.org/10.1104/pp.16.01420

A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis

A - Papers appearing in refereed journals
Piovesana, M., Wood, A. K. M., Smith, D. P., Deery, M. J., Bayliss, R., Carrera, E., Napier, J. A., Kurup, S. and Matthes, M. C. 2023. A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis. Journal of Experimental Botany. 74 (10), pp. 3104-3121. https://doi.org/10.1093/jxb/erad080

A stable start - cotranslational Nt-acetylation promotes proteome stability across kingdoms

A - Papers appearing in refereed journals
Gibbs, D. J., Bailey, M. and Etherington, R. D. 2022. A stable start - cotranslational Nt-acetylation promotes proteome stability across kingdoms. Trends in Cell biology. 32 (5), pp. 374-376. https://doi.org/10.1016/j.tcb.2022.02.004

A transatlantic perspective on 20 emerging issues in biological engineering

A - Papers appearing in refereed journals
Wintle, B. C., Boehm, C. R., Rhodes, C., Molloy J. C., Millett, P., Adam, L., Breitling, R., Carlson, R., Casogrande, R., Dando, M., Doubleday, R., Drexler, E., Edwards, B., Ellis, T., Evans, N. G., Hammond, R., Haseloff, J., Kahl, L., Kuiken, T., Lichman, B. R., Matthewman, C. A., Napier, J. A., Oheigeartaigh, S. S., Patron, N. J., Perello, E., Shapira, P., Tail, J., Takano, E and Sutherland, W. J. 2017. A transatlantic perspective on 20 emerging issues in biological engineering. eLife. 6 (e30247). https://doi.org/10.7554/elife.30247

Agriculture can help aquaculture become greener

A - Papers appearing in refereed journals
Napier, J. A., Haslam, R. P., Olsen, R-E., Tocher, D. R. and Betancor, M. B. 2020. Agriculture can help aquaculture become greener. Nature Food. 1, pp. 680-683. https://doi.org/10.1038/s43016-020-00182-9

Arabidopsis cytosolic acyl-CoA-binding proteins function in determining seed oil composition

A - Papers appearing in refereed journals
Guo, Z. H., Ye, Z. W., Haslam, R. P., Michaelson, L. V., Napier, J. A. and Chye, M. L. 2019. Arabidopsis cytosolic acyl-CoA-binding proteins function in determining seed oil composition. Plant Direct. 3 (12), p. e00182. https://doi.org/10.1002/pld3.182

Automated extraction of pod phenotype data from micro-computed tomograph

A - Papers appearing in refereed journals
Corcoran, E., Siles-Suarez, L., Kurup, S. and Ahnert, S. 2023. Automated extraction of pod phenotype data from micro-computed tomograph. Frontiers in Plant Science. 14, p. 1120182. https://doi.org/10.3389/fpls.2023.1120182

Basic LEUCINE ZIPPER TRANSCRIPTION FACTOR 67 transactivates DELAY OF GERMINATION 1 to establish primary seed dormancy in Arabidopsis

A - Papers appearing in refereed journals
Bryant, F. M., Hughes, D. J., Hassani-Pak, K. and Eastmond, P. J. 2019. Basic LEUCINE ZIPPER TRANSCRIPTION FACTOR 67 transactivates DELAY OF GERMINATION 1 to establish primary seed dormancy in Arabidopsis. The Plant Cell. https://doi.org/10.1105/tpc.18.00892

Beyond the one-way ANOVA for 'omics data

A - Papers appearing in refereed journals
Hassall, K. L. and Mead, A. 2018. Beyond the one-way ANOVA for 'omics data. BMC Bioinformatics. 19 (Suppl 7), p. 199. https://doi.org/10.1186/s12859-018-2173-7

BIG regulates dynamic adjustment of circadian period in Arabidopsis thaliana

A - Papers appearing in refereed journals
Hearn, T. J, Marti, M. C., Abdul-Awal, S. M., Wimalasekera, R., Stanton, C. R., Haydon, M. J., Theodoulou, F. L., Hannah, M. A. and Webb, A. A. R. 2018. BIG regulates dynamic adjustment of circadian period in Arabidopsis thaliana. Plant Physiology. 178, pp. 358-371. https://doi.org/10.1104/pp.18.00571

Chapter 11: The Properties, Distribution and Functionality of Cereal Lipids

B - Book chapters etc edited externally
Haslam, R. P. and Melis, S. 2023. Chapter 11: The Properties, Distribution and Functionality of Cereal Lipids. in: Shewry, P. R., Koshe, H. and Taylor, J. R. N (ed.) ICC Handbook of 21st Century Cereal Science and Technology Elsevier. pp. 103-110

Characterisation of Oil Palm Acyl-CoA-Binding Proteins and Correlation of their Gene Expression with Oil Synthesis

A - Papers appearing in refereed journals
Amiruddin, N., Chan, P-K., Azizi, N., Morris, P. E., Cham, K-L., Ong, P. W., Rosli, R., Masura, S. S., Murphy, D. J., Sambanthamurthi, R., Haslam, R. P., Chye, M-L., Harwood, J. L. and Low, E-T. L. 2019. Characterisation of Oil Palm Acyl-CoA-Binding Proteins and Correlation of their Gene Expression with Oil Synthesis. Plant and cell physiology. 61, pp. 735-747. https://doi.org/10.1093/pcp/pcz237

Chlamydomonas carries out fatty acid beta-oxidation in ancestral peroxisomes using a bona fide acyl-CoA oxidase

A - Papers appearing in refereed journals
Kong, F. T., Liang, Y. X., Legeret, B., Beyly-Adriano, A., Blangy, S., Haslam, R. P., Napier, J. A., Beisson, F., Peltier, G. and Li-Beisson, Y. 2017. Chlamydomonas carries out fatty acid beta-oxidation in ancestral peroxisomes using a bona fide acyl-CoA oxidase. The Plant Journal. 90 (2), pp. 358-371. https://doi.org/10.1111/tpj.13498

Defning lipids and T cell receptors involved in the intrinsic allergenicity of nut proteins

A - Papers appearing in refereed journals
Wang, R., Ghumra, A., Cochrane, S., Fairclough, L., Broughton, R., Michaelson, L. V., Beaudoin, F. and Alcocer, M. J. C. 2020. Defning lipids and T cell receptors involved in the intrinsic allergenicity of nut proteins. Clinical and Translational Allergy. 10, p. 54. https://doi.org/10.1186/s13601-020-00358-3

DES2 is a fatty acid Delta 11 desaturase capable of synthesizing palmitvaccenic acid in the arbuscular mycorrhizal fungus Rhizophagus irregularis

A - Papers appearing in refereed journals
Cheeld, H., Bhutada, G., Beaudoin, F. and Eastmond, P. J. 2020. DES2 is a fatty acid Delta 11 desaturase capable of synthesizing palmitvaccenic acid in the arbuscular mycorrhizal fungus Rhizophagus irregularis. FEBS Letters. https://doi.org/10.1002/1873-3468.13762

Development of a statistical crop model to explain the relationship between seed yield and phenotypic diversity within the Brassica napus genepool

A - Papers appearing in refereed journals
Bennett, E., Brignell, C., Carion, P. W. C., Cook, S. M., Eastmond, P. J., Teakle, G., Hammond, J. P., Love, C., King, G. J., Roberts, J. A. and Wagstaff, C. 2017. Development of a statistical crop model to explain the relationship between seed yield and phenotypic diversity within the Brassica napus genepool. Agronomy. 7 (2), p. 31. https://doi.org/10.3390/agronomy7020031

Dietary Supplementation with Transgenic Camelina sativa Oil Containing 20:5n-3 and 22:6n-3 or Fish Oil Induces Differential Changes in the Transcriptome of CD3+ T Lymphocytes

A - Papers appearing in refereed journals
West, A. L., Miles, E. A., Han, L., Lillycrop, K. A., Napier, J. A., Calder, P. C. and Burdge, G. C. 2021. Dietary Supplementation with Transgenic Camelina sativa Oil Containing 20:5n-3 and 22:6n-3 or Fish Oil Induces Differential Changes in the Transcriptome of CD3+ T Lymphocytes. Nutrients. 13 (9), p. 3116. https://doi.org/10.3390/nu13093116

Diverting phenylpropanoid pathway flux from sinapine to produce industrially useful 4-vinyl derivatives of hydroxycinnamic acids in Brassicaceous oilseeds

A - Papers appearing in refereed journals
Menard, G. N., Langdon, M., Bhunia, R., Shankhapal, A., Noleto-Dias, C., Lomax, C., Ward, J. L., Kurup, S. and Eastmond, P. J. 2022. Diverting phenylpropanoid pathway flux from sinapine to produce industrially useful 4-vinyl derivatives of hydroxycinnamic acids in Brassicaceous oilseeds. Metabolic Engineering. 70, pp. 196-205. https://doi.org/10.1016/j.ymben.2022.01.016

Effects of Cultivar and Nitrogen Nutrition on the Lipid Composition of Wheat Flour

A - Papers appearing in refereed journals
Min, B., Gonzalez-Thuillier, I., Powers, S. J., Wilde, P. J., Shewry, P. R. and Haslam, R. P. 2017. Effects of Cultivar and Nitrogen Nutrition on the Lipid Composition of Wheat Flour. Journal of Agricultural and Food Chemistry - JAFC. 65 (26), pp. 5427-5434. https://doi.org/10.1021/acs.jafc.7b01437

Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed

A - Papers appearing in refereed journals
Nogueira, M., Enfissi, E. M. A., Martínez Valenzuela, M. E., Menard, G. N., Driller, R. L., Eastmond, P. J., Schuch, W., Sandmann, G. and Fraser, P. D. 2017. Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed. Proceedings of the National Academy of Sciences. 114 (41), pp. 10876-10881. https://doi.org/10.1073/pnas.1708349114

Engineering the stereoisomeric structure of seed oil to mimic human milk fat

A - Papers appearing in refereed journals
Van-Erp, H., Bryant, F. M., Martin-Moreno, J., Michaelson, L. V., Bhutada, G. and Eastmond, P. J. 2019. Engineering the stereoisomeric structure of seed oil to mimic human milk fat. Proceedings of the National Academy of Sciences. 116 (42), pp. 20947-20952. https://doi.org/10.1073/pnas.1907915116

Enhancing the accumulation of eicosapentaenoic acid and docosahexaenoic acid in transgenic Camelina through the CRISPR-Cas9 inactivation of the competing FAE1 pathway

A - Papers appearing in refereed journals
Han, L., Haslam, R. P., Silvestre, S., Lu, C. and Napier, J. A. 2022. Enhancing the accumulation of eicosapentaenoic acid and docosahexaenoic acid in transgenic Camelina through the CRISPR-Cas9 inactivation of the competing FAE1 pathway. Plant Biotechnology Journal. 20 (8), pp. 1444-1446. https://doi.org/10.1111/pbi.13876

Ethylene augments root hypoxia tolerance through amelioration of reactive oxygen species and growth cessation

A - Papers appearing in refereed journals
Liu, Z., Hartman, S., Van Veen, H., Zhang, H., Leeggangers, H. A. C. F., Martopawiro, S., Bosman, F., De Deugd, F., Su, P., Hummel, M., Rankenberg, T., Hassall, K. L., Bailey-Serres, J., Theodoulou, F. L., Voesenek, L. A. C. J. and Sasidharan, R. 2022. Ethylene augments root hypoxia tolerance through amelioration of reactive oxygen species and growth cessation. bioRxiv. https://doi.org/10.1101/2022.01.21.477196

Ethylene augments root hypoxia tolerance via growth cessation and reactive oxygen species amelioration

A - Papers appearing in refereed journals
Liu, Z., Hartman, S., Van Veen, H., Zhang, H., Leeggangers , H. A. C. F., Martopawiro, S., Bosman, F., De Deugd, F., Su, P., Hummel, M., Rankenberg, T., Hassall, K. L., Bailey-Serres, J., Theodoulou, F. L., Voesenek L. A. C. J. and Sasidharan, R. 2022. Ethylene augments root hypoxia tolerance via growth cessation and reactive oxygen species amelioration. Plant Physiology. 190 (2), pp. 1365-1383. https://doi.org/10.1093/plphys/kiac245

Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress

A - Papers appearing in refereed journals
Hartman, S., Liu, Z., van Veen, H., Vicente, J., Reinen, E., Martopawiro, S., Zhang, H., van Dongen, N., Bosman, F., Bassel, G. W., Visser, E. J. W., Bailey-Serres, J., Theodoulou, F. L., Hebelstrup, K. H., Gibbs, D. J., Holdsworth, M. J., Sasidharan, R. and Vosenek, L. A. C. J. 2019. Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress. Nature Communications. 10 (4020). https://doi.org/10.1038/s41467-019-12045-4

Europe’s first and last field trial of gene-edited plants?

A - Papers appearing in refereed journals
Faure, J-D. and Napier, J. A. 2018. Europe’s first and last field trial of gene-edited plants? eLife. 7 (e42379). https://doi.org/10.7554/eLife.42379

Fatty acids in arbuscular mycorrhizal fungi are synthesized by the host plant

A - Papers appearing in refereed journals
Luginbuehl, L. H., Menard, G. N., Kurup, S., Van-Erp, H., Radhakrishnan, G. V., Breakspear, A., Oldroyd, G. E. D. and Eastmond, P. J. 2017. Fatty acids in arbuscular mycorrhizal fungi are synthesized by the host plant. Science - AAAS. 356 (6343), pp. 1175-1178. https://doi.org/10.1126/science.aan0081

Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment

A - Papers appearing in refereed journals
Zhang, H., Gannon, L., Jones, P. D., Rundle, C. A., Hassall, K. L., Gibbs, D. J., Holdsworth, M. J. and Theodoulou, F. L. 2018. Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment. Scientific Reports. 8, p. 15192. https://doi.org/10.1038/s41598-018-33630-5

Green light for lipid fingerprinting

A - Papers appearing in refereed journals
Haslam, R. P. and Feussner, I. 2017. Green light for lipid fingerprinting. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1862 (8), pp. 782-785. https://doi.org/10.1016/j.bbalip.2017.04.005

Identification of a Δ11 desaturase from the arbuscular mycorrhizal fungus Rhizophagus irregularis

A - Papers appearing in refereed journals
Cheeld, H., Bhutada, G., Beaudoin, F. and Eastmond, P. J. 2020. Identification of a Δ11 desaturase from the arbuscular mycorrhizal fungus Rhizophagus irregularis. bioRxiv. https://doi.org/10.1101/2020.01.13.903815

Impact of heat processing on the nutritional content of Gryllus bimaculatus (black cricket)

A - Papers appearing in refereed journals
Dobermann, D., Field, L. M. and Michaelson, L. V. 2019. Impact of heat processing on the nutritional content of Gryllus bimaculatus (black cricket). Nutrition Bulletin. pp. 1-7. https://doi.org/10.1111/nbu.12374

Intrinsic wheat lipid composition effects the interfacial and foaming properties of dough liquor

A - Papers appearing in refereed journals
Salt, L. J., Gonzalez-Thuillier, I., Chope, G., Penson, S., Tosi, P., Haslam, R. P., Skeggs, P. K., Shewry, P. R. and Wilde, P. J. 2017. Intrinsic wheat lipid composition effects the interfacial and foaming properties of dough liquor. Food Hydrocolloids. 75 (17 August), pp. 211-222. https://doi.org/10.1016/j.foodhyd.2017.08.020

Lipid remodelling: Unravelling the response to cold stress in Arabidopsis and its extremophile relative Eutrema salsugineum

A - Papers appearing in refereed journals
Barrero-Sicilia, C., Silvestre, S., Haslam, R. P. and Michaelson, L. V. 2017. Lipid remodelling: Unravelling the response to cold stress in Arabidopsis and its extremophile relative Eutrema salsugineum. Plant Science. 263 (October), pp. 194-200. https://doi.org/10.1016/j.plantsci.2017.07.017

Lipidomic Analysis of Plasma from Healthy Men and Women Shows Phospholipid Class and Molecular Species Differences between Sexes

A - Papers appearing in refereed journals
West, A. L., Michaelson, L. V., Miles, E. A., Haslam, R. P., Lillycrop, K. A., Georgescu, R., Han, L., Napier, J. A., Calder, P. C. and Burdge, G. C. 2020. Lipidomic Analysis of Plasma from Healthy Men and Women Shows Phospholipid Class and Molecular Species Differences between Sexes. Lipids. https://doi.org/10.1002/lipd.12293

Metabolic Engineering and Synthetic Biology Approaches to Enhancing Production of Long-Chain Polyunsaturated Fatty acids in Microalgae

B - Book chapters etc edited externally
Khozin-Goldberg, I. and Sayanova, O. V. 2020. Metabolic Engineering and Synthetic Biology Approaches to Enhancing Production of Long-Chain Polyunsaturated Fatty acids in Microalgae. in: Hallmann, A. and Rampelotto, P. (ed.) Grand Challenges in Algae Biotechnology. Grand Challenges in Biology and Biotechnology. Springer. pp. 249-289

Modulation of lipid biosynthesis by stress in diatoms

A - Papers appearing in refereed journals
Sayanova, O. V., Mimouni, V., Ulmann, L., Morant-Manceau, A., Pasquet, V., Schoefs, B. and Napier, J. A. 2017. Modulation of lipid biosynthesis by stress in diatoms. Philosophical Transactions of the Royal Society B-Biological Sciences. 372 (1728), pp. 1-14. https://doi.org/10.1098/rstb.2016.0407

Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase

A - Papers appearing in refereed journals
Pudney, A., Gandini, C., Economou, C., Smith, Richard T., Goddard, P., Napier, J. A., Spicer, A. and Sayanova, O. V. 2019. Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase. Scientific Reports. 9 (1), p. 11444. https://doi.org/10.1038/s41598-019-47875-1

Mutagenesis separates ATPase and thioesterase activities of the peroxisomal ABC transporter, Comatose

A - Papers appearing in refereed journals
Carrier, D., van Roermund, C., Schaedler, T. A., Rong, H., Ijlist, L., Wanders, r., Baldwin, S., Waterham, H., Theodoulou, F. L. and Baker, A. 2019. Mutagenesis separates ATPase and thioesterase activities of the peroxisomal ABC transporter, Comatose. Scientific Reports. 9. https://doi.org/10.1038/s41598-019-46685-9