Jackie Freeman

Research outputs

Response of cell wall composition and RNA-seq transcriptome to methyl-jasmonate in Brachypodium distachyon callus

Hyde, L. S., Pellny, T. K., Freeman, J., Michaelson, L. V., Simister, R., McQueen-Mason, S. J. and Mitchell, R. A. C. 2018. Response of cell wall composition and RNA-seq transcriptome to methyl-jasmonate in Brachypodium distachyon callus . Planta.

Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility

De Souza, W. R., Martins, P. K., Freeman, J., Pellny, T. K., Michaelson, L. V., Sampaio, B. L., Vinecky, F., Ribeiro, A. P., Da Cunha, B. A. D. B., Kobayashi, A. K., De Oliveira, P. A., Campanha, R. B., Pacheco, T. F., Martarello, D. C. I., Marchiosi, R., Ferrarese-Filho, O., Dos Santos, W. D., Tramontina, R., Squina, F. M., Centeno, D. C., Gaspar, M., Braga, M. R., Tine, M. A. S., Ralph, J., Mitchell, R. A. C. and Molinari, H. B. C. 2018. Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility. New Phytologist. 218 (1), pp. 81-93.

Development of wheat with low-viscosity grain extracts using novel alleles of IRX9 orthologue TaGT43_2

Pellny, T. K., Patil, A., Freeman, J., Lovegrove, A., Plummer, A., Hess, L., Shewry, P. R., Phillips, A., Berry, S. and Mitchell, R. 2017. Development of wheat with low-viscosity grain extracts using novel alleles of IRX9 orthologue TaGT43_2. Quality & Nutrition at MonoGram 2017, 4-6 April 2017, University of Bristol, Bristol p8 Oral Presentation. Bristol, University of Bristol. pp. 8

Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration

Freeman, J., Ward, J. L., Kosik, O., Lovegrove, A., Wilkinson, M. D., Shewry, P. R. and Mitchell, R. A. C. 2017. Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration. Plant Biotechnology Journal. 15 (11).

Effect of suppression of arabinoxylan synthetic genes in wheat endosperm on chain length of arabinoxylan and extract viscosity

Freeman, J., Lovegrove, A., Wilkinson, M. D., Saulnier, L., Shewry, P. R. and Mitchell, R. A. C. 2016. Effect of suppression of arabinoxylan synthetic genes in wheat endosperm on chain length of arabinoxylan and extract viscosity. Plant Biotechnology Journal. 14 (1), pp. 109-116.

Improving wheat as a source of dietary fibre for human health

Shewry, P. R., Reynolds, S., Pellny, T. K., Freeman, J., Wilkinson, M. D., Kosik, O., Ulker, M., Wingen, L., Orford, S., Griffiths, S., Mitchell, R. A. C. and Lovegrove, A. 2015. Improving wheat as a source of dietary fibre for human health. Cambridge University Press (CUP) Cambridge. pp. E87

Optimising the content and composition of dietary fibre in wheat grain for end-use quality

Shewry, P. R., Saulnier, L., Gebruers, K., Mitchell, R. A. C., Freeman, J., Nemeth, C. and Ward, J. L. 2014. Optimising the content and composition of dietary fibre in wheat grain for end-use quality. in: Tuberosaetal, R. (ed.) Genomics of plant genetic resources Springer, Dordrecht. pp. 455-466

RNA interference suppression of genes in glycosyl transferase families 43 and 47 in wheat starchy endosperm causes large decreases in arabinoxylan content

Lovegrove, A., Wilkinson, M. D., Freeman, J., Pellny, T. K., Tosi, P., Saulnier, L., Shewry, P. R. and Mitchell, R. A. C. 2013. RNA interference suppression of genes in glycosyl transferase families 43 and 47 in wheat starchy endosperm causes large decreases in arabinoxylan content. Plant Physiology. 163, pp. 95-107.

Grass cell wall feruloylation: distribution of bound ferulate and candidate gene expression in Brachypodium distachyon

Molinari, H. B. C., Pellny, T. K., Freeman, J., Shewry, P. R. and Mitchell, R. A. C. 2013. Grass cell wall feruloylation: distribution of bound ferulate and candidate gene expression in Brachypodium distachyon. Frontiers in Plant Science. 4, p. 50 (10pp).

Exploiting genetic variation to increase the dietary fibre content of wheat grain and flour

Shewry, P. R., Ulker, M., Lovegrove, A., Freeman, J., Pellny, T. K., Mitchell, R. A. C., Gebruers, K. and Rakszegi, M. 2012. Exploiting genetic variation to increase the dietary fibre content of wheat grain and flour. Lang, L. and Bedo, Z. (ed.) Plant Breeding for Future Generations: Proceedings 19th EUCARPIA General Congress, Budapest, 21-24 May 2012 . Agricultural Institute, Martonvasar. pp. 171-175

An integrated study of grain development of wheat (cv. Hereward)

Shewry, P. R., Mitchell, R. A. C., Tosi, P., Wan, Y., Toscano-Underwood, C., Lovegrove, A., Freeman, J., Toole, G. A., Mills, E. N. C. and Ward, J. L. 2012. An integrated study of grain development of wheat (cv. Hereward). Journal of Cereal Science. 56, pp. 21-30.

Cell walls of developing wheat starchy endosperm: comparison of composition and RNA-Seq transcriptome

Pellny, T. K., Lovegrove, A., Freeman, J., Tosi, P., Love, C. G., Knox, J. P., Shewry, P. R. and Mitchell, R. A. C. 2012. Cell walls of developing wheat starchy endosperm: comparison of composition and RNA-Seq transcriptome. Plant Physiology. 158, pp. 612-627.

Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses

Anders, N., Wilkinson, M. D., Lovegrove, A., Freeman, J., Tryfona, T., Pellny, T. K., Weimar, T., Mortimer, J. C., Stott, K., Baker, J. M., Defoin-Platel, M., Shewry, P. R., Dupree, P. and Mitchell, R. A. C. 2012. Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses. Proceedings of the National Academy of Sciences of the United States of America. 109 (3), pp. 989-993.

Temporal and spatial transgene expression using a heat-shock promoter in transgenic wheat

Freeman, J., Sparks, C. A., West, J. S., Shewry, P. R. and Jones, H. D. 2011. Temporal and spatial transgene expression using a heat-shock promoter in transgenic wheat. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 85-91

Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat

Freeman, J., Sparks, C. A., West, J., Shewry, P. R. and Jones, H. D. 2011. Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotechnology Journal. 9 (7), pp. 788-796.

Challenges and opportunities for using wheat for biofuel production

Shewry, P. R., Freeman, J., Wilkinson, M. D., Pellny, T. K. and Mitchell, R. A. C. 2011. Challenges and opportunities for using wheat for biofuel production. in: Karp, A. and Halford, N. G. (ed.) Energy crops Royal Society of Chemistry (RSC), Cambridge. pp. 13-26

Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging

Tosi, P., Gritsch, C., Freeman, J., Sparks, C. A., Jones, H. D., Funatsuki, W., Niwa, K. and Shewry, P. R. 2010. Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging. Branlard, G. (ed.) Gluten Proteins 2009: Proceedings 10th International Gluten Workshop, Clermont-Ferrand, 7-9 September 2009 . Institut National de la Recherche Agronomique (INRA), Paris. pp. 17-20

Temporal and spatial changes in cell wall composition in developing grains of wheat cv. Hereward

Toole, G. A., Le Gall, G., Colquhoun, I. J., Nemeth, C., Saulnier, L., Lovegrove, A., Pellny, T. K., Wilkinson, M. D., Freeman, J., Mitchell, R. A. C., Mills, E. N. C. and Shewry, P. R. 2010. Temporal and spatial changes in cell wall composition in developing grains of wheat cv. Hereward. Planta. 232, pp. 677-689.

Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat

Nemeth, C., Freeman, J., Jones, H. D., Sparks, C. A., Pellny, T. K., Wilkinson, M. D., Dunwell, J., Andersson, A. A. M., Aman, P., Guillon, F., Saulnier, L., Mitchell, R. A. C. and Shewry, P. R. 2010. Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat. Plant Physiology. 152, pp. 1209-1218.

Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging

Tosi, P., Gritsch, C., Freeman, J., Sparks, C. A., Jones, H. D., Funatsuki, W., Niwa, K. and Shewry, P. R. 2009. Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging. Abstracts Xth International Gluten Workshop, Clermont Ferrand, 7-9 September 2009 .

Improving wheat for nutrition and processing

Shewry, P. R., Wan, Y., Wheeler, T., Gooding, M., Poole, R., Edwards, K. J., Mills, E. N. C., Skeggs, P., Gezan, S., Welham, S. J., Shenton, C., Zhu, T., Phillips, A. L., Jones, H. D., Li, L., Ward, J. L., Freeman, J., Nemeth, C. and Mitchell, R. A. C. 2008. Improving wheat for nutrition and processing. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 249-252

Manipulation of the cell wall composition of wheat endosperm cell walls to improve nutritional properties

Nemeth, C., Freeman, J., Mitchell, R. A. C. and Shewry, P. R. 2008. Manipulation of the cell wall composition of wheat endosperm cell walls to improve nutritional properties. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 295-297

A transgenic approach to understanding gene expression in cereals

Jones, H. D., Freeman, J. and Sparks, C. A. 2008. A transgenic approach to understanding gene expression in cereals. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 427-430

Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm

Freeman, J., Kurup, S., Huttly, A. K. and Shewry, P. R. 2008. Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 453-455

The origin and early development of wheat glutenin particles

Van Herpen, T. W. J. M., Cordewener, J. H. G., Klok, H. J., Freeman, J., America, A. H. P., Bosch, D., Smulders, M. J. M., Gilissen, L. J. W. J., Shewry, P. R. and Hamer, R. J. 2008. The origin and early development of wheat glutenin particles. Journal of Cereal Science. 48, pp. 870-877.

Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm

Freeman, J., Kurup, S., Huttly, A. K. and Shewry, P. R. 2007. Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm. Abstracts 4th UK Small Grain Cereals Genetic and Genomics Workshop: From Genes to Traits, Cambridge, 14-16 March 2007 .

Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins

Shewry, P. R., Tosi, P., Jones, H. D., Sparks, C. A., Gritsch, C., Funatsuki, W., Niwa, K., Huttly, A. K., Napier, J. A., D'ovidio, R. and Freeman, J. 2007. Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins. Lookhart, G. L. and Ng, P. W. K. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 112-116

Using cell lineage tagging to decipher early differentiation and development in wheat endosperm

Freeman, J., Kurup, S. and Shewry, P. R. 2005. Using cell lineage tagging to decipher early differentiation and development in wheat endosperm. Abstracts 7th International Wheat Conference, Mar del Plata, 27 November - 2 December 2005 .

Methods for studying population structure, including sensitivity to the fungicide silthiofam, of the cereal take-all fungus, Gaeumannomyces graminis var. tritici

Freeman, J., Ward, E., Gutteridge, R. J. and Bateman, G. L. 2005. Methods for studying population structure, including sensitivity to the fungicide silthiofam, of the cereal take-all fungus, Gaeumannomyces graminis var. tritici. Plant Pathology. 54 (5), pp. 686-698.

Changes in protein secondary structure during gluten deformation studied by dynamic Fourier transform infrared spectroscopy

Wellner, N., Mills, E. N. C., Brownsey, G., Wilson, R. H., Brown, N. A., Freeman, J., Halford, N. G., Shewry, P. R. and Belton, P. S. 2005. Changes in protein secondary structure during gluten deformation studied by dynamic Fourier transform infrared spectroscopy. Biomacromolecules. 6 (1), pp. 255-261.

Structure and biomechanical properties of the gluten proteins deposited in the developing wheat grain: relationship to functional properties at harvest

Shewry, P. R., Wellner, N., Mills, E. N. C., Brownsey, G., Wilson, R. H., Brown, N. A., Freeman, J., Halford, N. G. and Belton, P. S. 2004. Structure and biomechanical properties of the gluten proteins deposited in the developing wheat grain: relationship to functional properties at harvest. Black, C. K., Rebetzke, G. J. and Panozzo, J. F. (ed.) Cereals 2004: Proceedings 54th Australian Cereal Chemistry Conference and 11th Wheat Breeders' Assembly, Canberra, 21-24 September 2004 . Royal Australian Chemical Institute, Melbourne. pp. 377-379

Gaeumannomyces graminis , the take-all fungus and its relatives

Freeman, J. and Ward, E. 2004. Gaeumannomyces graminis , the take-all fungus and its relatives. Molecular Plant Pathology. 5 (4), pp. 235-252.

Detecting airborne inoculum of fungal pathogens of oilseed rape using polymerase chain reaction (PCR) assays

McCartney, H. A., Freeman, J., Calderon, C., Foster, S. J. and Ward, E. 2003. Detecting airborne inoculum of fungal pathogens of oilseed rape using polymerase chain reaction (PCR) assays. Proceedings 11th International Rapeseed Congress, Copenhagen, 6-10 July 2003 . pp. 750-752

Petal fall, petal retention and petal duration in oilseed rape crops

McCartney, H. A., Heran, A., Li, Q., Freeman, J. and Foster, S. J. 2003. Petal fall, petal retention and petal duration in oilseed rape crops. Proceedings 11th International Rapeseed Congress, Copenhagen, 6-10 July 2003 . pp. 1084-1086

Infection of oilseed rape (Brassica napus ) by petal containing ascospores of Sclerotinia sclerotiorum

McCartney, H. A., Heran, A., Li, Q., Freeman, J. and Foster, S. J. 2003. Infection of oilseed rape (Brassica napus ) by petal containing ascospores of Sclerotinia sclerotiorum. Proceedings 11th International Rapeseed Congress, Copenhagen, 6-10 July 2003 . pp. 1987-1090

Polymerase chain reaction (PCR)-based assays for the detection of inoculum of Sclerotinia sclerotiorum spp

McCartney, H. A., Freeman, J., Foster, S. J. and Ward, E. 2003. Polymerase chain reaction (PCR)-based assays for the detection of inoculum of Sclerotinia sclerotiorum spp. Proceedings 11th International Rapeseed Congress, Copenhagen, 6-10 July 2003 . pp. 1090-1092

Strategies for fungicidal control of take-all. (H-GCA Project Report No. 309)

Bateman, G. L., Freeman, J., Gutteridge, R. J., Jenkyn, J. F. and Ward, E. 2003. Strategies for fungicidal control of take-all. (H-GCA Project Report No. 309). Home Grown Cereals Authority (HGCA) London.

Detecting airborne inoculum of fungal pathogens of oilseed rape by polymerase chain reaction assays

McCartney, H. A., Freeman, J., Calderon, C., Foster, S. J. and Ward, E. 2003. Detecting airborne inoculum of fungal pathogens of oilseed rape by polymerase chain reaction assays. Abstracts 8th International Congress of Plant Pathology, Christchurch, 2-7 February 2003 . pp. 6

Polymerase chain reaction based assays for the detection of inoculum of Sclerotinia sclerotiorum

Freeman, J., Calderon, C., Ward, E. and McCartney, H. A. 2003. Polymerase chain reaction based assays for the detection of inoculum of Sclerotinia sclerotiorum. Abstracts 8th International Congress of Plant Pathology, Christchurch, 2-7 February 2003 . pp. 90

A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum

Freeman, J., Ward, E., Calderon, C. and McCartney, H. A. 2002. A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum. European Journal of Plant Pathology. 108, pp. 877-886.

Detection of airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae in oilseed rape crops by polymerase chain reaction (PCR) assays

Calderon, C., Ward, E., Freeman, J., Foster, S. J. and McCartney, H. A. 2002. Detection of airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae in oilseed rape crops by polymerase chain reaction (PCR) assays. Plant Pathology. 51 (3), pp. 303-310.

Detection of airborne fungal spores sampled by rotating-arm and Hirst-type spore traps using polymerase chain reaction assays

Calderon, C., Ward, E., Freeman, J. and McCartney, H. A. 2002. Detection of airborne fungal spores sampled by rotating-arm and Hirst-type spore traps using polymerase chain reaction assays. Journal of Aerosol Science. 33, pp. 283-296.

Petal fall, petal retention and petal duration in oilseed rape crops

McCartney, H. A., Heran, A., Li, Q. and Freeman, J. 2001. Petal fall, petal retention and petal duration in oilseed rape crops. Abstracts Sclerotinia 2001 - 11th International Sclerotinia Workshop, York, 8-12 July 2001 . pp. 183-184

Polymerase chain reaction (PCR)-based assays for the detection of inoculum of Sclerotinia spp

Freeman, J., Calderon, C., Ward, E. and McCartney, H. A. 2001. Polymerase chain reaction (PCR)-based assays for the detection of inoculum of Sclerotinia spp. Abstracts Sclerotinia 2001 - 11th International Sclerotinia Workshop, York, 8-12 July 2001 . pp. 81-82

Detecting airborne inoculum of fungal pathogens of oilseed Brassicas by polymerase chain reaction (PCR) assays

McCartney, H. A., Freeman, J., Calderon, C. and Ward, E. 2001. Detecting airborne inoculum of fungal pathogens of oilseed Brassicas by polymerase chain reaction (PCR) assays. Abstracts 8th International Workshop on Plant Disease Epidemiology, Ouro Preto, Brazil, 6-11 May 2001 . pp. 144

The detection of airborne plant pathogen fungal spores by polymerase chain reaction (PCR) assays

Calderon, C., Freeman, J., McCartney, H. A. and Ward, E. 2000. The detection of airborne plant pathogen fungal spores by polymerase chain reaction (PCR) assays. Abtsracts 2nd European Symposium on Aerobiology, Vienna, 4-9 September 2000 . pp. M110

Analisis molecular de mutantes fotorrespiratorios de cebada deficientes en la biosintesis de glutamato

Marquez, A. J., Freeman, J., Avila, C., Wallsgrove, R. M., Pajuelo, P., Pajuelo, E. and Forde, B. G. 1992. Analisis molecular de mutantes fotorrespiratorios de cebada deficientes en la biosintesis de glutamato. Pineda, M. and Castillo, F. (ed.) Metabolismo del nitrogeno. Sociedad Espanola de Bioquimica, Madrid. pp. 140-144

Molecular analysis of barley mutants deficient in chloroplast glutamine synthetase

Freeman, J., Marquez, A. J., Wallsgrove, R. M., Saarelainen, R. and Forde, B. G. 1990. Molecular analysis of barley mutants deficient in chloroplast glutamine synthetase. Plant Molecular Biology. 14, pp. 297-311.

Nuclear factors interact with conserved A/T-rich elements upstream of a nodule-enhanced glutamine synthetase gene from French bean.

Forde, B. G., Freeman, J., Oliver, J. E. and Pineda, M. 1990. Nuclear factors interact with conserved A/T-rich elements upstream of a nodule-enhanced glutamine synthetase gene from French bean. The Plant Cell. 2, pp. 925-939.

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