Reference gene selection for quantitative RT-PCR normalisation in barley under low nitrogen

Chen, Z., Li, Y., Liu, C., Wang, Y., He, T., Guo, G., Fang, C., Gao, R., Xu, H., Zhou, L., +3 more...Huang, J., Halford, Nigel

and Lu, R. (2018) Reference gene selection for quantitative RT-PCR normalisation in barley under low nitrogen. Journal of Cereal Science, 82 (July). pp. 213-215. 10.1016/j.jcs.2018.06.014

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The aim of the present study was to identify genes that could be used for the normalisation of qRT-PCR data in barley, using RNAseq data (NCBI accession number PRJNA400519: RNA sequencing of Hordeum vulgare L. under low nitrogen stress). The particular interest was in genes that were stably expressed under different nitrogen conditions, including low-nitrogen stress.

Item Type	Article
Open Access	Gold
Keywords	barley; reference gene; qRT-PCR; low-nitrogen stress
Project	DFW - Designing Future Wheat - Work package 1 (WP1) - Increased efficiency and sustainability
Date Deposited	05 Dec 2025 09:11
Last Modified	19 Dec 2025 14:10