Molecular images of cereal proteins by STM
Thomson, N. H., Miles, M. J., Tatham, A. S. and Shewry, Peter
(1992)
Molecular images of cereal proteins by STM.
Ultramicroscopy, 42-44 (2).
pp. 1204-1213.
10.1016/0304-3991(92)90425-J
Scanning tunnelling microscopy (STM) has been used to study a seed storage protein of wheat known as y-gliadin. The protein was deposited onto highly oriented pyrolytic graphite (HOPG) from solutions of trifluoroethanol (TFE) and 1% acetic acid. Samples were dried down and then scanned in air. Transmission electron microscopy (TEM) was also used to visualise the distribution of protein on the substrate. Small-angle X-ray scattering (SAXS) was used to compare the molecular size and shape obtained with those from the STM images
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