An analysis of variability in genome organisation of intracellular calcium release channels across insect orders

A - Papers appearing in refereed journals

Troczka, B. J., Richardson, E., Homem, R. A. and Davies, T. G. E. 2018. An analysis of variability in genome organisation of intracellular calcium release channels across insect orders. Gene. 670 (5 September), pp. 70-86.

AuthorsTroczka, B. J., Richardson, E., Homem, R. A. and Davies, T. G. E.
Abstract

Using publicly available genomic data, combined with RT-PCR validation, we explore structural genomic variation for two major ion channels across insect classes. We have manually curated ryanodine receptor (RyR) and inositol 1,4,5-trisphosphate receptor (IP3R) ORFs and their corresponding genomic structures from 26 different insects covering major insect orders. We found that, despite high protein identity for both RyRs (> 75%) and IP3Rs (~67%), the overall complexity of the gene structure varies greatly between different insect orders with the simplest genes (fewest introns) found in Diptera and the most complex in Lepidoptera. Analysis of intron conservation patterns indicated that the majority of conserved introns are found close to the 5′ end of the channels and in RyR around the highly conserved mutually exclusive splice site. Of the two channels the IP3Rs appear to have a less well conserved organisation with a greater overall number of unique introns seen between insect orders. We experimentally validated two of the manually curated ORFs for IP3Rs and confirmed an atypical (3799aa) IP3R receptor in Myzus persicae, which is approximately 1000 amino acids larger than previously reported for IP3Rs.

KeywordsRyanodine receptor; Inositol 1,4,5-trisphosphate receptor ; Insect
Year of Publication2018
JournalGene
Journal citation670 (5 September), pp. 70-86
Digital Object Identifier (DOI)doi:10.1016/j.gene.2018.05.075
PubMed ID29792951
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Bayer Crop Science
Funder project or codeBBSRC Strategic Programme in Smart Crop Protection
Investigation and characterisation of diamide insecticide resistance due to target site mutations in the ryanodine receptor of lepidopteran pests
Pathogen surveillance and monitoring
Publisher's version
Copyright license
CC BY
Supplemental file
Copyright license
CC BY
Output statusPublished
Publication dates
Online21 May 2018
Publication process dates
Accepted18 May 2018
PublisherElsevier Science Bv
ISSN0378-1119

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