In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease

A - Papers appearing in refereed journals

Cordova, I., Jones, P., Harrison, N. A. and Oropeza, C. 2003. In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease. Molecular Plant Pathology. 4 (2), pp. 99-108. https://doi.org/10.1046/j.1364-3703.2003.00152.x

AuthorsCordova, I., Jones, P., Harrison, N. A. and Oropeza, C.
Abstract

DNA of the lethal yellowing (LY) phytoplasma was detected in 13 of 72 embryos from fruits of four diseased Atlantic tall coconut palms by polymerase chain reaction (PCR) assays employing phytoplasma universal rRNA primer pair P1/P7, nested LY group-specific rRNA primer pair 503f/LY16Sr or LY phytoplasma-specific nonribosomal primer pair. LYF1/R1. Phytoplasma distribution in sectioned tissues from six PCR positive embryos was determined by in situ PCR and digoxigenin-11-deoxy-UTP (Dig) labelling of amplification products. Dig-labeled DNA products detected by colourimetric assay were clearly evident on sections from the same three embryos investigated in detail by in situ PCRs employing primer pairs P1/P7 or LYF1/R1. Deposition of blue-green stain on sections as a result of each assay was restricted to areas of the embryos corresponding to the plumule and cells ensheathing it. By comparison, similarly treated embryo sections derived from fruits of a symptomless Atlantic tall coconut palm were consistently devoid of any stain. Presence of phytoplasma DNA in embryo tissues suggests the possible potential for seed transmission which remains to be demonstrated.

KeywordsPlant Sciences
Year of Publication2003
JournalMolecular Plant Pathology
Journal citation4 (2), pp. 99-108
Digital Object Identifier (DOI)https://doi.org/10.1046/j.1364-3703.2003.00152.x
PubMed ID20569368
Open accessPublished as non-open access
Funder project or code423
507
Characterisation and diagnosis of viruses affecting tropical crops
ISSN14646722
PublisherWiley

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