Identification of Primula fatty acid delta6-desaturases with n-3 substrate preferences

A - Papers appearing in refereed journals

Sayanova, O. V., Beaudoin, F., Michaelson, L. V., Shewry, P. R. and Napier, J. A. 2003. Identification of Primula fatty acid delta6-desaturases with n-3 substrate preferences. FEBS Letters. 542 (1-3), pp. 100-104.

AuthorsSayanova, O. V., Beaudoin, F., Michaelson, L. V., Shewry, P. R. and Napier, J. A.
Abstract

Fatty acid Δ6‐desaturation, the first committed step in C20 polyunsaturated fatty acid biosynthesis, is generally considered not to discriminate between n‐3 and n‐6 substrates. We previously identified higher plant species that showed preferential Δ6‐desaturation of n‐3 C18 fatty acid substrates. A polymerase chain reaction‐based approach was used to isolate ‘front‐end’ cytochrome b 5 fusion desaturases from Primula vialii Franchet and Primula farinosa L. Functional analysis in Saccharomyces cerevisiae identified fatty acid Δ6‐desaturases with a strong specificity for the n‐3 substrate α‐linolenic acid (18:3Δ9,12,15). These results indicate that the accumulation of octadecatetraenoic acid (18:4Δ6,9,12,15) in planta is due to the activity of a novel n‐3‐specific fatty acid Δ6‐desaturase. 

Year of Publication2003
JournalFEBS Letters
Journal citation542 (1-3), pp. 100-104
Digital Object Identifier (DOI)doi:10.1016/S0014-5793(03)00358-2
Open accessPublished as non-open access
Funder project or code415
503
ISSN00145793
PublisherWiley

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