A - Papers appearing in refereed journals
Shewry, P. R., Kreis, M., Parmar, S., Lew, E. J-L. and Kasarda, D. D. 1985. Identification of gamma-type hordeins in barley. FEBS Letters. 190 (1), pp. 61-64.
|Authors||Shewry, P. R., Kreis, M., Parmar, S., Lew, E. J-L. and Kasarda, D. D.|
The barley mutant Risϕ 56 has a deletion of at least 85 kb of DNA at the Hor 2 locus, resulting in the absence from the seeds of almost all of the major B hordein group of storage proteins. We have purified two fractions containing the three remaining ‘B hordein-like’ bands and determined their N-terminal amino acid sequences. One fraction, containing the two bands with higher Mr, gave a single major N-terminal sequence which was closely related to those reported for γ-type prolamins of wheat and rye. The sequence of the third band was also homologous with those of the γ-type prolamins, but less closely so. We consider that the γ-type prolamins are most closely related to the ancestral S-rich prolamins of the Triticeae, and this is the first report of their presence in barley.
|Keywords||Barley; Seed protein; Amino acid sequence; Homology|
|Year of Publication||1985|
|Journal citation||190 (1), pp. 61-64|
|Digital Object Identifier (DOI)||doi:10.1016/0014-5793(85)80427-0|
|Open access||Published as bronze (free) open access|
|Funder project or code||NATO Grant 342/82|
|07 Oct 1985|
|Copyright license||Publisher copyright|
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