A - Papers appearing in refereed journals
Shewry, P. R., Kreis, M., Parmar, S., Lew, E. J-L. and Kasarda, D. D. 1985. Identification of gamma-type hordeins in barley. FEBS Letters. 190 (1), pp. 61-64. https://doi.org/10.1016/0014-5793(85)80427-0
Authors | Shewry, P. R., Kreis, M., Parmar, S., Lew, E. J-L. and Kasarda, D. D. |
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Abstract | The barley mutant Risϕ 56 has a deletion of at least 85 kb of DNA at the Hor 2 locus, resulting in the absence from the seeds of almost all of the major B hordein group of storage proteins. We have purified two fractions containing the three remaining ‘B hordein-like’ bands and determined their N-terminal amino acid sequences. One fraction, containing the two bands with higher Mr, gave a single major N-terminal sequence which was closely related to those reported for γ-type prolamins of wheat and rye. The sequence of the third band was also homologous with those of the γ-type prolamins, but less closely so. We consider that the γ-type prolamins are most closely related to the ancestral S-rich prolamins of the Triticeae, and this is the first report of their presence in barley. |
Keywords | Barley; Seed protein; Amino acid sequence; Homology |
Year of Publication | 1985 |
Journal | FEBS Letters |
Journal citation | 190 (1), pp. 61-64 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/0014-5793(85)80427-0 |
Open access | Published as bronze (free) open access |
Funder | NATO |
Funder project or code | NATO Grant 342/82 |
Publisher's version | |
Output status | Published |
Publication dates | |
07 Oct 1985 | |
Copyright license | Publisher copyright |
Publisher | FEBS Press |
ISSN | 1873-3468 |
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