Identification of gamma-type hordeins in barley

A - Papers appearing in refereed journals

Shewry, P. R., Kreis, M., Parmar, S., Lew, E. J-L. and Kasarda, D. D. 1985. Identification of gamma-type hordeins in barley. FEBS Letters. 190 (1), pp. 61-64.

AuthorsShewry, P. R., Kreis, M., Parmar, S., Lew, E. J-L. and Kasarda, D. D.
Abstract

The barley mutant Risϕ 56 has a deletion of at least 85 kb of DNA at the Hor 2 locus, resulting in the absence from the seeds of almost all of the major B hordein group of storage proteins. We have purified two fractions containing the three remaining ‘B hordein-like’ bands and determined their N-terminal amino acid sequences. One fraction, containing the two bands with higher Mr, gave a single major N-terminal sequence which was closely related to those reported for γ-type prolamins of wheat and rye. The sequence of the third band was also homologous with those of the γ-type prolamins, but less closely so. We consider that the γ-type prolamins are most closely related to the ancestral S-rich prolamins of the Triticeae, and this is the first report of their presence in barley.

KeywordsBarley; Seed protein; Amino acid sequence; Homology
Year of Publication1985
JournalFEBS Letters
Journal citation190 (1), pp. 61-64
Digital Object Identifier (DOI)doi:10.1016/0014-5793(85)80427-0
Open accessPublished as bronze (free) open access
FunderNATO
Funder project or codeNATO Grant 342/82
Publisher's version
Output statusPublished
Publication dates
Print07 Oct 1985
Copyright licensePublisher copyright
PublisherFEBS Press
ISSN1873-3468

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