Surveys reveal the occurrence of phytoplasmas in plants at different geographical locations in Peru

A - Papers appearing in refereed journals

Hodgetts, J., Chuquillangui, C., Muller, G., Arocha, Y., Gamarra, D., Pinillos, O., Velit, E., Lozada, P., Boa, E., Boonham, N., Mumford, R., Barker, I. and Dickinson, M. 2009. Surveys reveal the occurrence of phytoplasmas in plants at different geographical locations in Peru. Annals of Applied Biology - AAB. 155 (1), pp. 15-27. https://doi.org/10.1111/j.1744-7348.2009.00316.x

AuthorsHodgetts, J., Chuquillangui, C., Muller, G., Arocha, Y., Gamarra, D., Pinillos, O., Velit, E., Lozada, P., Boa, E., Boonham, N., Mumford, R., Barker, I. and Dickinson, M.
Abstract

Two independent surveys were performed in Peru during February and November 2007 to detect the presence of phytoplasmas within any crops showing symptoms resembling those caused by phytoplasmas. Molecular identifications and characterisations were based on phytoplasma 16S and 23S rRNA genes using nested PCR and terminal restriction fragment length polymorphism (T-RFLP). The surveys indicated that phytoplasmas were present in most of the locations sampled in Peru in both cultivated crops, including carrots, maize, native potatoes, improved potato, tomato, oats, papaya and coconut, and in other plants such as dandelion and the ornamental Madagascar periwinkle (Catharanthus roseus). Phylogenetic analysis of the sequences confirmed that while most of the isolates belong to the 16SrI aster yellows group, which is ubiquitous throughout other parts of South America, one isolate from potato belongs to the 16SrII peanut witches' broom group, and one isolate from tomato and one from dandelion belong to the 16SrIII X-disease group. The use of T-RFLP was validated for the evaluation of phytoplasma-affected field samples and provided no evidence for mixed infection of individual plants with more than one phytoplasma isolate. These data represent the first molecular confirmation of the presence of phytoplasmas in a broad range of crops in Peru.

KeywordsAgriculture, Multidisciplinary
Year of Publication2009
JournalAnnals of Applied Biology - AAB
Journal citation155 (1), pp. 15-27
Digital Object Identifier (DOI)https://doi.org/10.1111/j.1744-7348.2009.00316.x
Open accessPublished as non-open access
FunderDEFRA - Department for Environment, Food and Rural Affairs UK
University of Nottingham
Rothamsted Research
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
Characterisation and diagnosis of viruses affecting tropical crops
ISSN00034746
PublisherWiley
Grant IDPHL 174B/4612 (09/2003)
12/2005
PHL 173B/5244

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