Structure and heterogeneity of gliadin: a hydrodynamic evaluation

A - Papers appearing in refereed journals

Ang, S., Kogulanathan, J., Morris, G. A., Kok, M. S., Shewry, P. R., Tatham, A. S., Adams, G. G., Rowe, A. J. and Harding, S. E. 2010. Structure and heterogeneity of gliadin: a hydrodynamic evaluation. European Biophysics Journal. 39 (January), pp. 255-261.

AuthorsAng, S., Kogulanathan, J., Morris, G. A., Kok, M. S., Shewry, P. R., Tatham, A. S., Adams, G. G., Rowe, A. J. and Harding, S. E.
Abstract

A study of the heterogeneity and conformation in solution [in 70% (v/v) aq. ethanol] of gliadin proteins from wheat was undertaken based upon sedimentation velocity in the analytical ultracentrifuge, analysis of the distribution coefficients and ellipsoidal axial ratios assuming quasi-rigid particles, allowing for a range of plausible time-averaged hydration values. All classical fractions (α, γ, ωslow, ωfast) show three clearly resolved components. Based on the weight-average sedimentation coefficient for each fraction and a weight-average molecular weight from sedimentation equilibrium and/or cDNA sequence analysis, all the proteins are extended molecules with axial ratios ranging from ~10 to 30 with α appearing the most extended and γ the least.

KeywordsGliadin ; Sedimentation coefficient; Molecular weight; Heterogeneity; Axial ratio; Extended conformation
Year of Publication2010
JournalEuropean Biophysics Journal
Journal citation39 (January), pp. 255-261
Digital Object Identifier (DOI)doi:10.1007/s00249-009-0529-7
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCentre for Crop Genetic Improvement (CGI)
Cereal seed composition and end use quality
Publisher's version
Copyright license
Publisher copyright
File Access Level
Restricted
Accepted author manuscript
Copyright license
CC BY
File Access Level
Open
Output statusPublished
Publication dates
Online08 Aug 2009
Publication process dates
Accepted22 Jul 2009
PublisherSpringer
ISSN0175-7571

Permalink - https://repository.rothamsted.ac.uk/item/8q438/structure-and-heterogeneity-of-gliadin-a-hydrodynamic-evaluation

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