A - Papers appearing in refereed journals
Ang, S., Kogulanathan, J., Morris, G. A., Kok, M. S., Shewry, P. R., Tatham, A. S., Adams, G. G., Rowe, A. J. and Harding, S. E. 2010. Structure and heterogeneity of gliadin: a hydrodynamic evaluation. European Biophysics Journal. 39 (January), pp. 255-261. https://doi.org/10.1007/s00249-009-0529-7
Authors | Ang, S., Kogulanathan, J., Morris, G. A., Kok, M. S., Shewry, P. R., Tatham, A. S., Adams, G. G., Rowe, A. J. and Harding, S. E. |
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Abstract | A study of the heterogeneity and conformation in solution [in 70% (v/v) aq. ethanol] of gliadin proteins from wheat was undertaken based upon sedimentation velocity in the analytical ultracentrifuge, analysis of the distribution coefficients and ellipsoidal axial ratios assuming quasi-rigid particles, allowing for a range of plausible time-averaged hydration values. All classical fractions (α, γ, ωslow, ωfast) show three clearly resolved components. Based on the weight-average sedimentation coefficient for each fraction and a weight-average molecular weight from sedimentation equilibrium and/or cDNA sequence analysis, all the proteins are extended molecules with axial ratios ranging from ~10 to 30 with α appearing the most extended and γ the least. |
Keywords | Gliadin ; Sedimentation coefficient; Molecular weight; Heterogeneity; Axial ratio; Extended conformation |
Year of Publication | 2010 |
Journal | European Biophysics Journal |
Journal citation | 39 (January), pp. 255-261 |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s00249-009-0529-7 |
Open access | Published as green open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Centre for Crop Genetic Improvement (CGI) |
Cereal seed composition and end use quality | |
Publisher's version | Copyright license Publisher copyright File Access Level Restricted |
Accepted author manuscript | Copyright license CC BY File Access Level Open |
Output status | Published |
Publication dates | |
Online | 08 Aug 2009 |
Publication process dates | |
Accepted | 22 Jul 2009 |
Publisher | Springer |
ISSN | 0175-7571 |
Permalink - https://repository.rothamsted.ac.uk/item/8q438/structure-and-heterogeneity-of-gliadin-a-hydrodynamic-evaluation
Publisher's version