Direct real-time imaging of protein adsorption onto hydrophilic and hydrophobic surfaces

A - Papers appearing in refereed journals

Haward, S. J., Shewry, P. R., Miles, M. J. and Mcmaster, T. J. 2010. Direct real-time imaging of protein adsorption onto hydrophilic and hydrophobic surfaces. Biopolymers. 93 (1), pp. 74-84.

AuthorsHaward, S. J., Shewry, P. R., Miles, M. J. and Mcmaster, T. J.
Abstract

Atomic force microscopy has been used to follow in real time the adsorption from solution of two of the gliadin group of wheat seed storage proteins onto hydrophilic (mica) and hydrophobic (graphite) surfaces. The liquid cell of the microscope was used initially to acquire images of the substrate under a small quantity of pure solvent (1% acetic acid). Continuous imaging as an injection of gliadin solution entered the liquid cell enabled the adsorption process to be followed in situ from zero time. For omega-gliadin, a monolayer was formed on the mica substrate during a period of similar to 2000 s, with the protein molecules oriented in parallel to the mica surface. In contrast, the w-gliadin had a relatively low affinity for the graphite substrate, as demonstrated by slow and weak adsorption to the surface., With gamma-gliadin, random deposition onto the mica surface was observed forming monodispersed structures, whereas on the graphite surface, monolayer islands of protein were formed with! the protein molecules in a perpendicular orientation. Sequential adsorption experiments indicated strong interactions between the two proteins that, under certain circumstances, caused alterations to the surface morphologies of preadsorbed species. The results are relevant to our understanding of the interactions of proteins within the hydrated protein bodies of wheat grain and how these determine the processing properties of wheat gluten and dough. (C) 2009 Wiley Periodicals, Inc. Biopolymers 93: 74-84, 2010.

KeywordsBiochemistry & Molecular Biology; Biophysics
Year of Publication2010
JournalBiopolymers
Journal citation93 (1), pp. 74-84
Digital Object Identifier (DOI)doi:10.1002/bip.21300
PubMed ID19728361
Open accessPublished as non-open access
Funder project or codeCGI
FunderBiotechnology and Biological Sciences Research Council
PublisherWiley
Grant IDBBS/E/C/00004953
ISSN0006-3525

Permalink - https://repository.rothamsted.ac.uk/item/8q44y/direct-real-time-imaging-of-protein-adsorption-onto-hydrophilic-and-hydrophobic-surfaces

Restricted files

Publisher's version

Under embargo indefinitely

4 total views
2 total downloads
2 views this month
1 downloads this month