A - Papers appearing in refereed journals
Freeman, J., Sparks, C. A., West, J., Shewry, P. R. and Jones, H. D. 2011. Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotechnology Journal. 9 (7), pp. 788-796.
|Authors||Freeman, J., Sparks, C. A., West, J., Shewry, P. R. and Jones, H. D.|
Constitutive promoters are widely used to functionally characterise plant genes in transgenic plants, but their lack of specificity and poor control over protein expression can be a major disadvantage. On the other hand, promoters that provide precise regulation of temporal or spatial transgene expression facilitate such studies by targeting over-expression or knockdown of target genes to specific tissues and/or at particular developmental stages. Here, we used the uidA (beta-glucuronidase, GUS) reporter gene to demonstrate that the barley Hvhsp17 gene promoter can be induced by heat treatment of 38-40 degrees C for 1-2 h in transgenic wheat. The GUS enzyme was expressed only in those tissues directly exposed to heat and not in neighbouring leaf tissues. The induction of HSP:: GUS was demonstrated in all organs and tissues tested, but expression in older tissues was lower. Generally, proximal root sections showed less GUS activity than in root tips. This heat-inducible promoter provides the ability to investigate the function of candidate genes by overexpression or by down-regulation of target gene expression (for example by RNAi) in selected tissues or developmental stages of a transgenic plant, limited only by the ability to apply a heat shock to the selected tissues. It also allows the investigation of genes that would be lethal or reduce fertility if expressed constitutively.
|Keywords||Biotechnology & Applied Microbiology; Plant Sciences|
|Year of Publication||2011|
|Journal||Plant Biotechnology Journal|
|Journal citation||9 (7), pp. 788-796|
|Digital Object Identifier (DOI)||doi:10.1111/j.1467-7652.2011.00588.x|
|Open access||Published as green open access|
|Funder||Biotechnology and Biological Sciences Research Council|
|Funder project or code||CGI|
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