Insecticide resistance status of Myzus persicae in Greece: long-term surveys and new diagnostics for resistance mechanisms

A - Papers appearing in refereed journals

Voudouris, C. C., Kati, A. N., Sadikoglou, E., Williamson, M., Skouras, P. J., Dimotsiou, O., Georgiou, S., Fenton, B., Skavdis, G. and Margaritopoulos, J. T. 2016. Insecticide resistance status of Myzus persicae in Greece: long-term surveys and new diagnostics for resistance mechanisms. Pest Management Science. 72 (4), pp. 671-683. https://doi.org/10.1002/ps.4036

AuthorsVoudouris, C. C., Kati, A. N., Sadikoglou, E., Williamson, M., Skouras, P. J., Dimotsiou, O., Georgiou, S., Fenton, B., Skavdis, G. and Margaritopoulos, J. T.
Abstract

BACKGROUNDMyzus persicae nicotianae is an important pest in Greece, controlled mainly by neonicotinoids. Monitoring of the aphid populations for resistance mechanisms is essential for effective control. RESULTSTwo new RFLP-based diagnostics for the detection of the M918T (super-kdr pyrethroid resistance) and nAChR R81T (neonicotinoid resistance) mutations were applied, along with other established assays, on 131 nicotianae multilocus genotypes (MLGs) collected from tobacco and peach in Greece in 2012-2013. Furthermore, we present resistance data from aphid clones (>500, mainly nicotianae) collected in 2006-2007. About half of the clones tested with a diagnostic dose of imidacloprid were tolerant. The R81T mutation was not found in the 131 MLGs and 152 clones examined. Over half (58.6%) of a subset of 29 clones showed a 9-36-fold overexpression of CYP6CY3. M918T was found at low to moderate frequencies. The kdr and MACE mechanisms and carboxylesterase-based resistance were found at high frequency in all years. CONCLUSIONThe aphid retains costly resistance mechanisms even in the absence of pressure from certain insecticides, which could be attributed to factors related to climate and genetic properties of the populations. The indication of build-up of resistance/tolerance to neonicotinoids, related to CYP6CY3 overexpression, is a matter of concern. (c) 2015 Society of Chemical Industry

KeywordsAgronomy; Entomology
Year of Publication2016
JournalPest Management Science
Journal citation72 (4), pp. 671-683
Digital Object Identifier (DOI)https://doi.org/10.1002/ps.4036
PubMed ID25960200
Open accessPublished as non-open access
FunderTHALIS project - Greek national funds through the Operational Programme 'Education and Lifelong Learning' of the National Strategic Reference Framework [380264]
programme 'Supporting Postdoctoral Researchers' - European Social Fund
programme 'Supporting Postdoctoral Researchers' - Hellenic State, General Secretariat of Research and Technology of Greece
Funder project or codeSustainability
PublisherWiley
ISSN1526-498X

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